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Effect of Cd2+ and Hg2+ on the activity of Na+/K+-ATPase and Mg2+-ATPase adsorbed on polystyrene microtiter plates
dc.creator | Vasić, Vesna M. | |
dc.creator | Jovanovic, D | |
dc.creator | Horvat, Anica | |
dc.creator | Momić, Tatjana | |
dc.creator | Nizezic, G | |
dc.date.accessioned | 2018-03-01T19:04:12Z | |
dc.date.available | 2018-03-01T19:04:12Z | |
dc.date.issued | 2002 | |
dc.identifier.issn | 0003-2697 | |
dc.identifier.uri | https://vinar.vin.bg.ac.rs/handle/123456789/2496 | |
dc.description.abstract | In the present study a polystyrene microtiter plate was tested as a support material for synaptic plasma membrane (SPM) immobilization by adsorption. The adsorption was carried out by an 18-h incubation at +4degreesC of SPM with a polystyrene matrix, at pH 7.4. Evaluation of the efficiency of the applied immobilization method revealed that 10% protein fraction of initially applied SPM was bound to the support and that two SPM enzymes, Na+/K+-ATPase and Mg2+-ATPase, retained 70-80% activity after the adsorption. In addition, adsorption stabilizes Na+/K+-ATPase and Mg2+-ATPase, since the activities are substantial 3 weeks after the adsorption. Parallel kinetic analysis showed that adsorption does not alter significantly the kinetic properties of Na+/K+-ATPase and Mg2+-ATPase and their sensitivity to and mechanism of Cd2+- or Hg2+- induced inhibition. The only exception is the high affinity Mg2+-ATPase moiety, whose affinity for ATP and sensitivity toward Cd2+ were increased by the adsorption. The results show that such system may be used as a practical and comfortable model for the in vitro toxicological investigations. (C) 2001 Elsevier Science. | en |
dc.rights | restrictedAccess | en |
dc.source | Analytical Biochemistry | en |
dc.subject | Na+/K+-ATPase | en |
dc.subject | Mg2+-ATPase | en |
dc.subject | synaptic plasma membranes | en |
dc.subject | Adsorption | en |
dc.subject | heavy metals | en |
dc.subject | kinetics | en |
dc.title | Effect of Cd2+ and Hg2+ on the activity of Na+/K+-ATPase and Mg2+-ATPase adsorbed on polystyrene microtiter plates | en |
dc.type | article | en |
dcterms.abstract | Низезиц, Г; Јовановиц, Д; Васић Весна М; Хорват, Aница; Момић Татјана; | |
dc.citation.volume | 300 | |
dc.citation.issue | 2 | |
dc.citation.spage | 113 | |
dc.citation.epage | 120 | |
dc.identifier.wos | 000173371000002 | |
dc.identifier.doi | 10.1006/abio.2001.5405 | |
dc.citation.rank | M21 | |
dc.identifier.pmid | 11779101 | |
dc.identifier.scopus | 2-s2.0-0037080992 |
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