Age dependence of T-lymphocyte apoptosis induced by high-energy proton exposure
Apstrakt
Peripheral blood samples from three donors of different ages were exposed to 300 kVp x-rays or 138 MeV protons (0, 2, and 9 Gy dose). After 48 h incubation, CD4 and CD8 T-lymphocytes were labelled with specific monoclonal antibodies and cellular DNA was stained by propidium iodine. Radiation-induced apoptosis was followed by flow cytometry and the data were processed by LYSIS II software. The data analysis revealed an age-dependent sensitivity to radiation-induced apoptosis by 300 kVp x-rays and 138 MeV protons, for both CD4 and CD8 T-lymphocytes. Radiation-induced apoptosis was about 4 times greater in CD4 lymphocytes from the youngest donor than the oldest donor and was about 2 times greater in CD8 T-lymphocytes, both after x-ray and proton exposures. RBE values for CD4 T-lymphocytes ranged from 0.9 to 1.4 and for CD8-positive cells from 0.7 to 0.9. It is concluded that radiation-induced apoptosis of CD4 and CD8 T-lymphocytes, which is already exploited to pi-edict patient response i...n conventional radiotherapy, may also be used to predict response in proton treatment planning.
Izvor:
Radiation and Environmental Biophysics, 2001, 40, 2, 131-135
DOI: 10.1007/s004110100093
ISSN: 0301-634X
PubMed: 11484784
WoS: 000169757500005
Scopus: 2-s2.0-0034958764
Kolekcije
Institucija/grupa
VinčaTY - JOUR AU - Radojčić, Marija AU - Crompton, NEA PY - 2001 UR - https://vinar.vin.bg.ac.rs/handle/123456789/2444 AB - Peripheral blood samples from three donors of different ages were exposed to 300 kVp x-rays or 138 MeV protons (0, 2, and 9 Gy dose). After 48 h incubation, CD4 and CD8 T-lymphocytes were labelled with specific monoclonal antibodies and cellular DNA was stained by propidium iodine. Radiation-induced apoptosis was followed by flow cytometry and the data were processed by LYSIS II software. The data analysis revealed an age-dependent sensitivity to radiation-induced apoptosis by 300 kVp x-rays and 138 MeV protons, for both CD4 and CD8 T-lymphocytes. Radiation-induced apoptosis was about 4 times greater in CD4 lymphocytes from the youngest donor than the oldest donor and was about 2 times greater in CD8 T-lymphocytes, both after x-ray and proton exposures. RBE values for CD4 T-lymphocytes ranged from 0.9 to 1.4 and for CD8-positive cells from 0.7 to 0.9. It is concluded that radiation-induced apoptosis of CD4 and CD8 T-lymphocytes, which is already exploited to pi-edict patient response in conventional radiotherapy, may also be used to predict response in proton treatment planning. T2 - Radiation and Environmental Biophysics T1 - Age dependence of T-lymphocyte apoptosis induced by high-energy proton exposure VL - 40 IS - 2 SP - 131 EP - 135 DO - 10.1007/s004110100093 ER -
@article{ author = "Radojčić, Marija and Crompton, NEA", year = "2001", abstract = "Peripheral blood samples from three donors of different ages were exposed to 300 kVp x-rays or 138 MeV protons (0, 2, and 9 Gy dose). After 48 h incubation, CD4 and CD8 T-lymphocytes were labelled with specific monoclonal antibodies and cellular DNA was stained by propidium iodine. Radiation-induced apoptosis was followed by flow cytometry and the data were processed by LYSIS II software. The data analysis revealed an age-dependent sensitivity to radiation-induced apoptosis by 300 kVp x-rays and 138 MeV protons, for both CD4 and CD8 T-lymphocytes. Radiation-induced apoptosis was about 4 times greater in CD4 lymphocytes from the youngest donor than the oldest donor and was about 2 times greater in CD8 T-lymphocytes, both after x-ray and proton exposures. RBE values for CD4 T-lymphocytes ranged from 0.9 to 1.4 and for CD8-positive cells from 0.7 to 0.9. It is concluded that radiation-induced apoptosis of CD4 and CD8 T-lymphocytes, which is already exploited to pi-edict patient response in conventional radiotherapy, may also be used to predict response in proton treatment planning.", journal = "Radiation and Environmental Biophysics", title = "Age dependence of T-lymphocyte apoptosis induced by high-energy proton exposure", volume = "40", number = "2", pages = "131-135", doi = "10.1007/s004110100093" }
Radojčić, M.,& Crompton, N.. (2001). Age dependence of T-lymphocyte apoptosis induced by high-energy proton exposure. in Radiation and Environmental Biophysics, 40(2), 131-135. https://doi.org/10.1007/s004110100093
Radojčić M, Crompton N. Age dependence of T-lymphocyte apoptosis induced by high-energy proton exposure. in Radiation and Environmental Biophysics. 2001;40(2):131-135. doi:10.1007/s004110100093 .
Radojčić, Marija, Crompton, NEA, "Age dependence of T-lymphocyte apoptosis induced by high-energy proton exposure" in Radiation and Environmental Biophysics, 40, no. 2 (2001):131-135, https://doi.org/10.1007/s004110100093 . .