Chemical aspects of the influence of cobalt ions on ATPase activity
Апстракт
The influence of Co2+ ions on the activities of Na+/K+-ATPase and Mg2+-ATPase, enzymes from rat brain synaptic plasma membrane, was studied. The aim of this study was to investigate the inhibition of both ATPases activities by exposure to cobalt ions as a function of experimentally added CoSO4. The free Co2+ concentrations in the reaction mixture were also calculated and discussed. CoSO4 induced a dose-dependent inhibition of both enzymes. The IC50 values of Co2+, as calculated from the experimental curves, were 168 mu M for Na+/K+-ATPase and 262 mu M for Mg2+-ATPase, and for the recalculated free Co2+ concentration 75.4 mu M for Na+/K+-ATPase and 136 mu M for Mg2+-ATPase. The obtained linear Dixons plot for Na+/K+-ATPase implies equilibrium binding of cobalt with inhibitory sites on the enzyme. The kinetic parameters for both enzymes in presence and absence of CoSO4 were calculated from the experimental data. The results of the kinetic analysis show that inhibition of Na+/K+-ATPase in...duced by CoSO4 is non-competitive, and for Mg2+-ATPAse that there are two sites of different sensitivities or two different enzymes.
Кључне речи:
cobalt / Na+/K+-ATPase / Mg2+-ATPase / kineticsИзвор:
Journal of the Serbian Chemical Society, 2000, 65, 7, 507-515Колекције
Институција/група
VinčaTY - JOUR AU - Vujisić Lj. AU - Krstić, Danijela Z. AU - Vucetic, J PY - 2000 UR - https://vinar.vin.bg.ac.rs/handle/123456789/2364 AB - The influence of Co2+ ions on the activities of Na+/K+-ATPase and Mg2+-ATPase, enzymes from rat brain synaptic plasma membrane, was studied. The aim of this study was to investigate the inhibition of both ATPases activities by exposure to cobalt ions as a function of experimentally added CoSO4. The free Co2+ concentrations in the reaction mixture were also calculated and discussed. CoSO4 induced a dose-dependent inhibition of both enzymes. The IC50 values of Co2+, as calculated from the experimental curves, were 168 mu M for Na+/K+-ATPase and 262 mu M for Mg2+-ATPase, and for the recalculated free Co2+ concentration 75.4 mu M for Na+/K+-ATPase and 136 mu M for Mg2+-ATPase. The obtained linear Dixons plot for Na+/K+-ATPase implies equilibrium binding of cobalt with inhibitory sites on the enzyme. The kinetic parameters for both enzymes in presence and absence of CoSO4 were calculated from the experimental data. The results of the kinetic analysis show that inhibition of Na+/K+-ATPase induced by CoSO4 is non-competitive, and for Mg2+-ATPAse that there are two sites of different sensitivities or two different enzymes. T2 - Journal of the Serbian Chemical Society T1 - Chemical aspects of the influence of cobalt ions on ATPase activity VL - 65 IS - 7 SP - 507 EP - 515 UR - https://hdl.handle.net/21.15107/rcub_vinar_2364 ER -
@article{ author = "Vujisić Lj. and Krstić, Danijela Z. and Vucetic, J", year = "2000", abstract = "The influence of Co2+ ions on the activities of Na+/K+-ATPase and Mg2+-ATPase, enzymes from rat brain synaptic plasma membrane, was studied. The aim of this study was to investigate the inhibition of both ATPases activities by exposure to cobalt ions as a function of experimentally added CoSO4. The free Co2+ concentrations in the reaction mixture were also calculated and discussed. CoSO4 induced a dose-dependent inhibition of both enzymes. The IC50 values of Co2+, as calculated from the experimental curves, were 168 mu M for Na+/K+-ATPase and 262 mu M for Mg2+-ATPase, and for the recalculated free Co2+ concentration 75.4 mu M for Na+/K+-ATPase and 136 mu M for Mg2+-ATPase. The obtained linear Dixons plot for Na+/K+-ATPase implies equilibrium binding of cobalt with inhibitory sites on the enzyme. The kinetic parameters for both enzymes in presence and absence of CoSO4 were calculated from the experimental data. The results of the kinetic analysis show that inhibition of Na+/K+-ATPase induced by CoSO4 is non-competitive, and for Mg2+-ATPAse that there are two sites of different sensitivities or two different enzymes.", journal = "Journal of the Serbian Chemical Society", title = "Chemical aspects of the influence of cobalt ions on ATPase activity", volume = "65", number = "7", pages = "507-515", url = "https://hdl.handle.net/21.15107/rcub_vinar_2364" }
Vujisić Lj., Krstić, D. Z.,& Vucetic, J.. (2000). Chemical aspects of the influence of cobalt ions on ATPase activity. in Journal of the Serbian Chemical Society, 65(7), 507-515. https://hdl.handle.net/21.15107/rcub_vinar_2364
Vujisić Lj., Krstić DZ, Vucetic J. Chemical aspects of the influence of cobalt ions on ATPase activity. in Journal of the Serbian Chemical Society. 2000;65(7):507-515. https://hdl.handle.net/21.15107/rcub_vinar_2364 .
Vujisić Lj., Krstić, Danijela Z., Vucetic, J, "Chemical aspects of the influence of cobalt ions on ATPase activity" in Journal of the Serbian Chemical Society, 65, no. 7 (2000):507-515, https://hdl.handle.net/21.15107/rcub_vinar_2364 .