17 beta-Estradiol in vitro affects Na-dependent and depolarization-induced Ca2+ transport in rat brain synaptosomes
Апстракт
Effects of 17 beta-estradiol (E(2)) in vitro on Na-dependent Ca2+ efflux from, and depolarization-induced Ca2+ uptake into, the nerve cell were studied with the use of synaptosomes isolated from the brain stem, mesencephalic reticular formation (MRF), caudate nucleus and the hippocampus of long-term ovariectomized adult female rats. It was found that E(2) (1) at a concentration of 10 nM or lower, stimulates Na-dependent Ca2+ efflux in the caudate nucleus and hippocampus, and does not affect the efflux in MRF and brain stem; (2) at concentrations above 10 nM has no effect on the Ca2+ efflux in any of the four structures investigated; and (3) produces a biphasic effect on the depolarization-induced Ca2+ uptake, increasing it in all structures except MRF at 10 nM concentration, and decreasing it at concentrations higher than 10 nM, irrespective of the structure investigated. These results suggest that E(2), acting at extranuclear sites, modulates synaptic transmission via alterations of C...a2+ transport mechanisms in nerve endings.
Кључне речи:
estradiol / calcium transport / synaptosomes / brain / ratИзвор:
Experientia, 1996, 52, 3, 217-220
DOI: 10.1007/BF01920709
ISSN: 0014-4754
PubMed: 8631388
WoS: A1996UC55500003
Scopus: 2-s2.0-0029670177
Институција/група
VinčaTY - JOUR AU - Nikezić, Gordana S. AU - Horvat, Anica AU - Nedeljković, N. AU - Martinović, Jelena PY - 1996 UR - https://vinar.vin.bg.ac.rs/handle/123456789/1968 AB - Effects of 17 beta-estradiol (E(2)) in vitro on Na-dependent Ca2+ efflux from, and depolarization-induced Ca2+ uptake into, the nerve cell were studied with the use of synaptosomes isolated from the brain stem, mesencephalic reticular formation (MRF), caudate nucleus and the hippocampus of long-term ovariectomized adult female rats. It was found that E(2) (1) at a concentration of 10 nM or lower, stimulates Na-dependent Ca2+ efflux in the caudate nucleus and hippocampus, and does not affect the efflux in MRF and brain stem; (2) at concentrations above 10 nM has no effect on the Ca2+ efflux in any of the four structures investigated; and (3) produces a biphasic effect on the depolarization-induced Ca2+ uptake, increasing it in all structures except MRF at 10 nM concentration, and decreasing it at concentrations higher than 10 nM, irrespective of the structure investigated. These results suggest that E(2), acting at extranuclear sites, modulates synaptic transmission via alterations of Ca2+ transport mechanisms in nerve endings. T2 - Experientia T1 - 17 beta-Estradiol in vitro affects Na-dependent and depolarization-induced Ca2+ transport in rat brain synaptosomes VL - 52 IS - 3 SP - 217 EP - 220 DO - 10.1007/BF01920709 UR - https://hdl.handle.net/21.15107/rcub_vinar_1968 ER -
@article{ author = "Nikezić, Gordana S. and Horvat, Anica and Nedeljković, N. and Martinović, Jelena", year = "1996", abstract = "Effects of 17 beta-estradiol (E(2)) in vitro on Na-dependent Ca2+ efflux from, and depolarization-induced Ca2+ uptake into, the nerve cell were studied with the use of synaptosomes isolated from the brain stem, mesencephalic reticular formation (MRF), caudate nucleus and the hippocampus of long-term ovariectomized adult female rats. It was found that E(2) (1) at a concentration of 10 nM or lower, stimulates Na-dependent Ca2+ efflux in the caudate nucleus and hippocampus, and does not affect the efflux in MRF and brain stem; (2) at concentrations above 10 nM has no effect on the Ca2+ efflux in any of the four structures investigated; and (3) produces a biphasic effect on the depolarization-induced Ca2+ uptake, increasing it in all structures except MRF at 10 nM concentration, and decreasing it at concentrations higher than 10 nM, irrespective of the structure investigated. These results suggest that E(2), acting at extranuclear sites, modulates synaptic transmission via alterations of Ca2+ transport mechanisms in nerve endings.", journal = "Experientia", title = "17 beta-Estradiol in vitro affects Na-dependent and depolarization-induced Ca2+ transport in rat brain synaptosomes", volume = "52", number = "3", pages = "217-220", doi = "10.1007/BF01920709", url = "https://hdl.handle.net/21.15107/rcub_vinar_1968" }
Nikezić, G. S., Horvat, A., Nedeljković, N.,& Martinović, J.. (1996). 17 beta-Estradiol in vitro affects Na-dependent and depolarization-induced Ca2+ transport in rat brain synaptosomes. in Experientia, 52(3), 217-220. https://doi.org/10.1007/BF01920709 https://hdl.handle.net/21.15107/rcub_vinar_1968
Nikezić GS, Horvat A, Nedeljković N, Martinović J. 17 beta-Estradiol in vitro affects Na-dependent and depolarization-induced Ca2+ transport in rat brain synaptosomes. in Experientia. 1996;52(3):217-220. doi:10.1007/BF01920709 https://hdl.handle.net/21.15107/rcub_vinar_1968 .
Nikezić, Gordana S., Horvat, Anica, Nedeljković, N., Martinović, Jelena, "17 beta-Estradiol in vitro affects Na-dependent and depolarization-induced Ca2+ transport in rat brain synaptosomes" in Experientia, 52, no. 3 (1996):217-220, https://doi.org/10.1007/BF01920709 ., https://hdl.handle.net/21.15107/rcub_vinar_1968 .