dc.creator | Perić, Ivana | |
dc.creator | Stanisavljević, Andrijana | |
dc.creator | Gass, Peter | |
dc.creator | Filipović, Dragana | |
dc.date.accessioned | 2018-03-01T17:57:45Z | |
dc.date.available | 2018-03-01T17:57:45Z | |
dc.date.issued | 2017 | |
dc.identifier.issn | 0940-1334 | |
dc.identifier.issn | 1433-8491 | |
dc.identifier.uri | https://vinar.vin.bg.ac.rs/handle/123456789/1806 | |
dc.description.abstract | Exposure of an organism to chronic social isolation (CSIS) has been shown to have an important role in depression. Fluoxetine (Flx) is a first-line treatment for depression; however, its downstream mechanisms of action beyond serotonergic signaling remain ill-defined. We investigated the effect of 3 weeks of Flx (15 mg/kg/day) treatment on behavioral changes and protein expression/activity of the GSH-dependent defense system, including reduced glutathione (GSH), glutathione peroxidase (GPx), glutathione reductase (GLR), and glutathione S-transferase (GST), as well as catalase (CAT), in the hippocampus of rats exposed to 6 weeks of CSIS. The subcellular distributions of nuclear factor-kappa B (NF-kappa B), as well as, cytosolic IL-1 beta and IL-6 protein expression, were also determined. CSIS induced depressive- and anxiety-like behaviors, evidenced by a decrease in sucrose preference and an increase in the number of buried marbles. Moreover, CSIS compromised redox homeostasis, targeting enzymes such as GPx, CAT, GST, and caused NF-kappa B nuclear translocation with a concomitant increase in IL-6 protein expression, without an effect on IL-1 beta. Flx treatment reversed CSIS-induced depressive- and anxiety-like behaviors, modulated GSH-dependent defense by increasing GLR and GST activity, and suppressed NF-kappa B activation and cytosolic IL-6 protein expression in socially isolated rats. The present study suggests that changes in the GSH-dependent defense system, NF-kappa B activation and increased IL-6 protein expression may have a role in social isolation-induced changes in a rat model of depression and anxiety, and contributes to our understanding of the mechanisms that underlie the antidepressant and anti-inflammatory activity of Flx in socially isolated rats. | en |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173044/RS// | |
dc.relation | Deutsche Forschungsgemeinschaft [SFB636-TP3] | |
dc.rights | restrictedAccess | en |
dc.source | European Archives of Psychiatry and Clinical Neuroscience | en |
dc.subject | Social isolation | en |
dc.subject | Fluoxetine | en |
dc.subject | Behavioral changes | en |
dc.subject | GSH-dependent defense system | en |
dc.subject | Proinflammatory cytokines | en |
dc.subject | Rat hippocampus | en |
dc.title | Fluoxetine reverses behavior changes in socially isolated rats: role of the hippocampal GSH-dependent defense system and proinflammatory cytokines | en |
dc.type | article | en |
dc.rights.license | ARR | |
dcterms.abstract | Станисављевиц, Aндријана; Перић Ивана; Гасс, Петер; Филиповић Драгана; | |
dc.citation.volume | 267 | |
dc.citation.issue | 8 | |
dc.citation.spage | 737 | |
dc.citation.epage | 749 | |
dc.identifier.wos | 000414154100004 | |
dc.identifier.doi | 10.1007/s00406-017-0807-9 | |
dc.citation.rank | M21 | |
dc.identifier.pmid | 28474231 | |
dc.type.version | publishedVersion | |
dc.identifier.scopus | 2-s2.0-85018738817 | |