Prikaz osnovnih podataka o dokumentu
Influence of a High-fat Diet on Cardiac iNOS in Female Rats
dc.creator | Jovanović, Aleksandra | |
dc.creator | Sudar, Emina | |
dc.creator | Obradović, Milan M. | |
dc.creator | Pitt, Samantha J. | |
dc.creator | Stewart, Alan J. | |
dc.creator | Zafirović, Sonja | |
dc.creator | Stanimirović, Julijana | |
dc.creator | Radak, Đorđe J. | |
dc.creator | Isenović, Esma R. | |
dc.date.accessioned | 2018-03-01T17:46:30Z | |
dc.date.available | 2018-03-01T17:46:30Z | |
dc.date.issued | 2017 | |
dc.identifier.issn | 1570-1611 | |
dc.identifier.issn | 1875-6212 | |
dc.identifier.uri | https://vinar.vin.bg.ac.rs/handle/123456789/1678 | |
dc.description.abstract | Background: Overexpression of inducible nitric oxide synthase (iNOS) is a key link between high-fat (HF) diet induced obesity and cardiovascular disease. Oestradiol has cardioprotective effects that may be mediated through reduction of iNOS activity/expression. Methods: In the present study, female Wistar rats were fed a standard diet or a HF diet (42% fat) for 10 weeks. iNOS gene and protein expressions were measured in heart tissue. HF-fed rats exhibited a significant increase in cardiac iNOS mRNA by 695% (p LT 0.05), iNOS protein level by 248% (p LT 0.01), without changes in nitrate/nitrite levels. Expression of CD36 protein in plasma membranes was increased by 37% (p LT 0.05), while the concentration of free fatty acids (FFA) was reduced by 25% (p LT 0.01) in HF-fed rats. Expression of the p50 subunit of nuclear factor-kappa B (NF kappa B-p50) in heart was increased by 77% (p LT 0.01) in HF-fed rats. Expression of protein kinase B (Akt) and extracellular signal-regulated kinases 1/2 (ERK1/2) were unchanged between the groups. There was a significant increase in the ratio of phospho-Akt/total Akt but not for phospho-ERK1/2/total ERK1/2 in HF-fed rats. Estrogen receptor-levels (by 50%; p LT 0.05) and serum oestradiol concentrations (by 35%; p LT 0.05) were shown to be significantly reduced in HF-fed rats. Results and Conclusion: Our results revealed that a HF diet led to increased iNOS expression, most likely via a mechanism involving Akt and NF kappa B-p50 proteins. Decreased levels of oestradiol and ER protein in the HF-fed group, in combination with increased iNOS levels are consistent with the hypothesis that oestradiol has a cardioprotective effect through its ability to regulate iNOS expression. | en |
dc.relation | info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/41002/RS// | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173033/RS// | |
dc.rights | restrictedAccess | en |
dc.source | Current Vascular Pharmacology | en |
dc.subject | Cardioprotection | en |
dc.subject | cardiovascular disease | en |
dc.subject | oestradiol | en |
dc.subject | inducible nitric oxide synthase | en |
dc.subject | insulin resistance | en |
dc.subject | obesity | en |
dc.title | Influence of a High-fat Diet on Cardiac iNOS in Female Rats | en |
dc.type | article | en |
dc.rights.license | ARR | |
dcterms.abstract | Радак, Дјордје; Јовановић Aлександра; Питт, Самантха Ј.; Судар Емина; Обрадовић Милан; Стеwарт, Aлан Ј.; Зафировић Соња; Станимировић Јулијана; Исеновић Есма; | |
dc.citation.volume | 15 | |
dc.citation.issue | 5 | |
dc.citation.spage | 491 | |
dc.citation.epage | 500 | |
dc.identifier.wos | 000407476700011 | |
dc.identifier.doi | 10.2174/1570161114666161025101303 | |
dc.citation.rank | M23 | |
dc.identifier.pmid | 27781956 | |
dc.type.version | publishedVersion | |
dc.identifier.scopus | 2-s2.0-85041327847 |
Dokumenti
Datoteke | Veličina | Format | Pregled |
---|---|---|---|
Uz ovaj zapis nema datoteka. |
Ovaj dokument se pojavljuje u sledećim kolekcijama
-
WoS Import
-
080 - Laboratorija za radiobiologiju i molekularnu genetiku
Department of Radiobiology and Molecular Genetics