The impact of concentration and administration time on the radiomodulating properties of undecylprodigiosin in vitro
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Undecylprodigiosin pigment (UPP) is reported to display cytotoxic activity towards various types of tumours. Nevertheless, its efficacy in modifying the cellular response to ionising radiation is still unknown. In this study, the radiomodulating effects of UPP were investigated. The effects of UPP were assessed in vitro by treating cultures of human peripheral blood with UPP and ionising radiation using two treatment regimens, the UPP pre-irradiation treatment and UPP post-irradiation treatment. The activity of UPP was investigated evaluating its effects on the radiation-induced micronuclei formation, cell proliferation, and induction of apoptosis. The redox modulating effects of UPP were examined measuring the catalase activity and the level of malondialdehyde, as a measure of oxidative stress. The results showed that UPP effects on cellular response to ionising radiation depend on its concentration and the timing of its administration. At low concentration, the UPP displayed radiopro...tective effects in. gamma-irradiated human lymphocytes while at higher concentrations, it acted as a radiosensitiser enhancing either mitotic catastrophe or apoptosis depending on the treatment regimen. The UPP modified redox processes in cells, particularly when it was employed prior to gamma-irradiation. Our data highlight the importance of further research of the potential of UPP to sensitize tumour cells to radiation therapy by inhibiting pathways that lead to treatment resistance.
Кључне речи:
apoptosis / mitotic catastrophe / oxidative stress / radiosensitisation / undecylprodigiosinИзвор:
Arhiv za higijenu rada i toksikologiju, 2017, 68, 1, 1-8Финансирање / пројекти:
- Истраживања интеракција ензима са токсичним и фармаколошки активним молекулима (RS-MESTD-Basic Research (BR or ON)-172023)
DOI: 10.1515/aiht-2017-68-2897
ISSN: 0004-1254; 1848-6312
PubMed: 28365670
WoS: 000399013400001
Scopus: 2-s2.0-85017372652
Колекције
Институција/група
VinčaTY - JOUR AU - Petrović, Sandra AU - Vasić, Vesna M. AU - Mitrović, Tatjana AU - Lazović, Saša AU - Leskovac, Andreja PY - 2017 UR - https://vinar.vin.bg.ac.rs/handle/123456789/1506 AB - Undecylprodigiosin pigment (UPP) is reported to display cytotoxic activity towards various types of tumours. Nevertheless, its efficacy in modifying the cellular response to ionising radiation is still unknown. In this study, the radiomodulating effects of UPP were investigated. The effects of UPP were assessed in vitro by treating cultures of human peripheral blood with UPP and ionising radiation using two treatment regimens, the UPP pre-irradiation treatment and UPP post-irradiation treatment. The activity of UPP was investigated evaluating its effects on the radiation-induced micronuclei formation, cell proliferation, and induction of apoptosis. The redox modulating effects of UPP were examined measuring the catalase activity and the level of malondialdehyde, as a measure of oxidative stress. The results showed that UPP effects on cellular response to ionising radiation depend on its concentration and the timing of its administration. At low concentration, the UPP displayed radioprotective effects in. gamma-irradiated human lymphocytes while at higher concentrations, it acted as a radiosensitiser enhancing either mitotic catastrophe or apoptosis depending on the treatment regimen. The UPP modified redox processes in cells, particularly when it was employed prior to gamma-irradiation. Our data highlight the importance of further research of the potential of UPP to sensitize tumour cells to radiation therapy by inhibiting pathways that lead to treatment resistance. T2 - Arhiv za higijenu rada i toksikologiju T1 - The impact of concentration and administration time on the radiomodulating properties of undecylprodigiosin in vitro VL - 68 IS - 1 SP - 1 EP - 8 DO - 10.1515/aiht-2017-68-2897 ER -
@article{ author = "Petrović, Sandra and Vasić, Vesna M. and Mitrović, Tatjana and Lazović, Saša and Leskovac, Andreja", year = "2017", abstract = "Undecylprodigiosin pigment (UPP) is reported to display cytotoxic activity towards various types of tumours. Nevertheless, its efficacy in modifying the cellular response to ionising radiation is still unknown. In this study, the radiomodulating effects of UPP were investigated. The effects of UPP were assessed in vitro by treating cultures of human peripheral blood with UPP and ionising radiation using two treatment regimens, the UPP pre-irradiation treatment and UPP post-irradiation treatment. The activity of UPP was investigated evaluating its effects on the radiation-induced micronuclei formation, cell proliferation, and induction of apoptosis. The redox modulating effects of UPP were examined measuring the catalase activity and the level of malondialdehyde, as a measure of oxidative stress. The results showed that UPP effects on cellular response to ionising radiation depend on its concentration and the timing of its administration. At low concentration, the UPP displayed radioprotective effects in. gamma-irradiated human lymphocytes while at higher concentrations, it acted as a radiosensitiser enhancing either mitotic catastrophe or apoptosis depending on the treatment regimen. The UPP modified redox processes in cells, particularly when it was employed prior to gamma-irradiation. Our data highlight the importance of further research of the potential of UPP to sensitize tumour cells to radiation therapy by inhibiting pathways that lead to treatment resistance.", journal = "Arhiv za higijenu rada i toksikologiju", title = "The impact of concentration and administration time on the radiomodulating properties of undecylprodigiosin in vitro", volume = "68", number = "1", pages = "1-8", doi = "10.1515/aiht-2017-68-2897" }
Petrović, S., Vasić, V. M., Mitrović, T., Lazović, S.,& Leskovac, A.. (2017). The impact of concentration and administration time on the radiomodulating properties of undecylprodigiosin in vitro. in Arhiv za higijenu rada i toksikologiju, 68(1), 1-8. https://doi.org/10.1515/aiht-2017-68-2897
Petrović S, Vasić VM, Mitrović T, Lazović S, Leskovac A. The impact of concentration and administration time on the radiomodulating properties of undecylprodigiosin in vitro. in Arhiv za higijenu rada i toksikologiju. 2017;68(1):1-8. doi:10.1515/aiht-2017-68-2897 .
Petrović, Sandra, Vasić, Vesna M., Mitrović, Tatjana, Lazović, Saša, Leskovac, Andreja, "The impact of concentration and administration time on the radiomodulating properties of undecylprodigiosin in vitro" in Arhiv za higijenu rada i toksikologiju, 68, no. 1 (2017):1-8, https://doi.org/10.1515/aiht-2017-68-2897 . .