Investigating an organ-targeting platform based on hydroxyapatite nanoparticles using a novel in situ method of radioactive (125)Iodine labeling
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© 2014 Elsevier B.V.
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In this study, we have investigated the synthesis of nanoparticles of hydroxyapatite (HAp) and hydroxyapatite coated with chitosan (HAp/Ch) and the chitosan-poly-D,L-lactide-co-glycolide polymer blend (HAp/Ch-PLGA) as an organ-targeting system. We have examined and defined the final destination, as well as the dynamics and the pathways of the synthesized particles following intravenous administration in vivo. The XRD, ZP, FT-IR and SEM analyses have confirmed that the hydroxyapatite nanoparticles with d(50) = 72 nm are coated with polymers. Radioactive 125-Iodine (I-125), a low energy gamma emitter, was used to develop a novel in situ method for the radiolabeling of particles and investigation of their biodistribution. I-125-labeled particles exhibited high stability in saline and serum over the second day, which justified their use in the following in vivo studies. The biodistribution of I-125-labeled particles after intravenous injection in rats differed significantly: HAp particles ...mostly targeted the liver, HAp/Ch the spleen and the liver, while HAp/Ch-PLGA targeted the lungs. Twenty-four hours post injection, HAp particles were excreted completely, while both I-125-HAp/Ch and I-125-HAp/Ch-PLGA were retained in the body for a prolonged period of time with more than 20% of radioactivity still found in different organs. (C) 2014 Elsevier B.V. All rights reserved.
Ključne reči:
Nano-hydroxyapatite / Chitosan / Organ-targeting / In situ I-125-labeling / BiodistributionIzvor:
Materials Science and Engineering. C: Materials for Biological Applications, 2014, 43, 439-446Finansiranje / projekti:
- Molekularno dizajniranje nanočestica kontrolisanih morfoloških i fizičko-hemijskih karakteristika i funkcionalnih materijala na njihovoj osnovi (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-45004)
Napomena:
- Peer-reviewed version available at http://vinar.vin.bg.ac.rs/handle/123456789/8602
DOI: 10.1016/j.msec.2014.07.046
ISSN: 0928-4931
PubMed: 25175234
WoS: 000342529000054
Scopus: 2-s2.0-84905381787
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Institucija/grupa
VinčaTY - JOUR AU - Ignjatović, Nenad L. AU - Vranješ-Đurić, Sanja AU - Mitić, Žarko AU - Janković, Drina AU - Uskoković, Dragan PY - 2014 UR - https://vinar.vin.bg.ac.rs/handle/123456789/139 AB - In this study, we have investigated the synthesis of nanoparticles of hydroxyapatite (HAp) and hydroxyapatite coated with chitosan (HAp/Ch) and the chitosan-poly-D,L-lactide-co-glycolide polymer blend (HAp/Ch-PLGA) as an organ-targeting system. We have examined and defined the final destination, as well as the dynamics and the pathways of the synthesized particles following intravenous administration in vivo. The XRD, ZP, FT-IR and SEM analyses have confirmed that the hydroxyapatite nanoparticles with d(50) = 72 nm are coated with polymers. Radioactive 125-Iodine (I-125), a low energy gamma emitter, was used to develop a novel in situ method for the radiolabeling of particles and investigation of their biodistribution. I-125-labeled particles exhibited high stability in saline and serum over the second day, which justified their use in the following in vivo studies. The biodistribution of I-125-labeled particles after intravenous injection in rats differed significantly: HAp particles mostly targeted the liver, HAp/Ch the spleen and the liver, while HAp/Ch-PLGA targeted the lungs. Twenty-four hours post injection, HAp particles were excreted completely, while both I-125-HAp/Ch and I-125-HAp/Ch-PLGA were retained in the body for a prolonged period of time with more than 20% of radioactivity still found in different organs. (C) 2014 Elsevier B.V. All rights reserved. T2 - Materials Science and Engineering. C: Materials for Biological Applications T1 - Investigating an organ-targeting platform based on hydroxyapatite nanoparticles using a novel in situ method of radioactive (125)Iodine labeling VL - 43 SP - 439 EP - 446 DO - 10.1016/j.msec.2014.07.046 ER -
@article{ author = "Ignjatović, Nenad L. and Vranješ-Đurić, Sanja and Mitić, Žarko and Janković, Drina and Uskoković, Dragan", year = "2014", abstract = "In this study, we have investigated the synthesis of nanoparticles of hydroxyapatite (HAp) and hydroxyapatite coated with chitosan (HAp/Ch) and the chitosan-poly-D,L-lactide-co-glycolide polymer blend (HAp/Ch-PLGA) as an organ-targeting system. We have examined and defined the final destination, as well as the dynamics and the pathways of the synthesized particles following intravenous administration in vivo. The XRD, ZP, FT-IR and SEM analyses have confirmed that the hydroxyapatite nanoparticles with d(50) = 72 nm are coated with polymers. Radioactive 125-Iodine (I-125), a low energy gamma emitter, was used to develop a novel in situ method for the radiolabeling of particles and investigation of their biodistribution. I-125-labeled particles exhibited high stability in saline and serum over the second day, which justified their use in the following in vivo studies. The biodistribution of I-125-labeled particles after intravenous injection in rats differed significantly: HAp particles mostly targeted the liver, HAp/Ch the spleen and the liver, while HAp/Ch-PLGA targeted the lungs. Twenty-four hours post injection, HAp particles were excreted completely, while both I-125-HAp/Ch and I-125-HAp/Ch-PLGA were retained in the body for a prolonged period of time with more than 20% of radioactivity still found in different organs. (C) 2014 Elsevier B.V. All rights reserved.", journal = "Materials Science and Engineering. C: Materials for Biological Applications", title = "Investigating an organ-targeting platform based on hydroxyapatite nanoparticles using a novel in situ method of radioactive (125)Iodine labeling", volume = "43", pages = "439-446", doi = "10.1016/j.msec.2014.07.046" }
Ignjatović, N. L., Vranješ-Đurić, S., Mitić, Ž., Janković, D.,& Uskoković, D.. (2014). Investigating an organ-targeting platform based on hydroxyapatite nanoparticles using a novel in situ method of radioactive (125)Iodine labeling. in Materials Science and Engineering. C: Materials for Biological Applications, 43, 439-446. https://doi.org/10.1016/j.msec.2014.07.046
Ignjatović NL, Vranješ-Đurić S, Mitić Ž, Janković D, Uskoković D. Investigating an organ-targeting platform based on hydroxyapatite nanoparticles using a novel in situ method of radioactive (125)Iodine labeling. in Materials Science and Engineering. C: Materials for Biological Applications. 2014;43:439-446. doi:10.1016/j.msec.2014.07.046 .
Ignjatović, Nenad L., Vranješ-Đurić, Sanja, Mitić, Žarko, Janković, Drina, Uskoković, Dragan, "Investigating an organ-targeting platform based on hydroxyapatite nanoparticles using a novel in situ method of radioactive (125)Iodine labeling" in Materials Science and Engineering. C: Materials for Biological Applications, 43 (2014):439-446, https://doi.org/10.1016/j.msec.2014.07.046 . .