Water as a Green Solvent for the Extraction of Polyphenols from Sage
Apstrakt
Sage (Salvia officinalis L.), a common member of the family Lamiaceae has been used as traditional herbal medicine against many diseases. It is reported that the plant has a variety of pharmacological effects, including anti- inflammatory, antibacterial, antiviral, hypoglycaemic, fungistatic, anticancer, antioxidative and antimutagenic effects. These effects are mainly due to the presence of phenolic compounds such as carnosic, rosmarinic, caffeic, salvianolic acids and flavonoids (luteolin-7-O-glucoside, quercetin and rutin). It is known that polyphenols are extracted with polar solvents, commonly with methanol, ethanol and their mixtures with water. Hence water is a polar solvent, it also can be considered as a potentially green solvent since it is non-toxic to health and the environment. In this study, we determined the total phenolic content (TPC) and total flavonoid content (TFC) in lyophilised extracts prepared with water extraction of sage leaves. The TPC of sage extracts (0.5 m...g/mL, 0.25 mg/mL and 0.125 mg/mL) was determined using a colorimetric assay, with Folin–Ciocalteu reagent. TPC was expressed as mg of gallic acid equivalents (GAE) per g of dried extract (de). The TFC of sage extracts (1.75 mg/mL, 1.25 mg/mL and 0.75 mg/mL) was measured by the spectrophotometric method, based on the formation of a flavonoid-aluminium complex. TFC was expressed as mg of quercetin equivalents (QE) per g of dried extract (de). The results were presented as a mean value ± standard deviation (SD) of triplicate measurements. Our findings showed that TPC is in the range from 155 ± 5.55 to 166 ± 2.95 mg GAE/g de. TFC was in range from 31.7 ± 3.61 to 46.6 ± 2.54 mg QE/g de. Compared with published results where extractions of polyphenols from sage were carried out with methanol as an effective and toxic solvent, results obtained in our study demonstrated a high extraction yield of polyphenols from sage using water as a polar and non-toxic solvent.
Ključne reči:
Sage / Green Extraction / Total Phenolic Content / Total Flavonoid Content / PolyphenolsIzvor:
DISC2023 : 3rd DIFENEW International Student Conference : Abstract book, 2023, 12-12Izdavač:
- Novi Sad : Faculty of Technical Sciences
Finansiranje / projekti:
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200017 (Univerzitet u Beogradu, Institut za nuklearne nauke Vinča, Beograd-Vinča) (RS-MESTD-inst-2020-200017)
Napomena:
- Third DIFENEW International Student Conference : 5th December 2023, Novi Sad.
Kolekcije
Institucija/grupa
VinčaTY - CONF AU - Janić, Marijana AU - Valenta Šobot, Ana AU - Momić, Tatjana AU - Filipović Tričković, Jelena PY - 2023 UR - https://vinar.vin.bg.ac.rs/handle/123456789/13333 AB - Sage (Salvia officinalis L.), a common member of the family Lamiaceae has been used as traditional herbal medicine against many diseases. It is reported that the plant has a variety of pharmacological effects, including anti- inflammatory, antibacterial, antiviral, hypoglycaemic, fungistatic, anticancer, antioxidative and antimutagenic effects. These effects are mainly due to the presence of phenolic compounds such as carnosic, rosmarinic, caffeic, salvianolic acids and flavonoids (luteolin-7-O-glucoside, quercetin and rutin). It is known that polyphenols are extracted with polar solvents, commonly with methanol, ethanol and their mixtures with water. Hence water is a polar solvent, it also can be considered as a potentially green solvent since it is non-toxic to health and the environment. In this study, we determined the total phenolic content (TPC) and total flavonoid content (TFC) in lyophilised extracts prepared with water extraction of sage leaves. The TPC of sage extracts (0.5 mg/mL, 0.25 mg/mL and 0.125 mg/mL) was determined using a colorimetric assay, with Folin–Ciocalteu reagent. TPC was expressed as mg of gallic acid equivalents (GAE) per g of dried extract (de). The TFC of sage extracts (1.75 mg/mL, 1.25 mg/mL and 0.75 mg/mL) was measured by the spectrophotometric method, based on the formation of a flavonoid-aluminium complex. TFC was expressed as mg of quercetin equivalents (QE) per g of dried extract (de). The results were presented as a mean value ± standard deviation (SD) of triplicate measurements. Our findings showed that TPC is in the range from 155 ± 5.55 to 166 ± 2.95 mg GAE/g de. TFC was in range from 31.7 ± 3.61 to 46.6 ± 2.54 mg QE/g de. Compared with published results where extractions of polyphenols from sage were carried out with methanol as an effective and toxic solvent, results obtained in our study demonstrated a high extraction yield of polyphenols from sage using water as a polar and non-toxic solvent. PB - Novi Sad : Faculty of Technical Sciences C3 - DISC2023 : 3rd DIFENEW International Student Conference : Abstract book T1 - Water as a Green Solvent for the Extraction of Polyphenols from Sage SP - 12 EP - 12 UR - https://hdl.handle.net/21.15107/rcub_vinar_13333 ER -
@conference{ author = "Janić, Marijana and Valenta Šobot, Ana and Momić, Tatjana and Filipović Tričković, Jelena", year = "2023", abstract = "Sage (Salvia officinalis L.), a common member of the family Lamiaceae has been used as traditional herbal medicine against many diseases. It is reported that the plant has a variety of pharmacological effects, including anti- inflammatory, antibacterial, antiviral, hypoglycaemic, fungistatic, anticancer, antioxidative and antimutagenic effects. These effects are mainly due to the presence of phenolic compounds such as carnosic, rosmarinic, caffeic, salvianolic acids and flavonoids (luteolin-7-O-glucoside, quercetin and rutin). It is known that polyphenols are extracted with polar solvents, commonly with methanol, ethanol and their mixtures with water. Hence water is a polar solvent, it also can be considered as a potentially green solvent since it is non-toxic to health and the environment. In this study, we determined the total phenolic content (TPC) and total flavonoid content (TFC) in lyophilised extracts prepared with water extraction of sage leaves. The TPC of sage extracts (0.5 mg/mL, 0.25 mg/mL and 0.125 mg/mL) was determined using a colorimetric assay, with Folin–Ciocalteu reagent. TPC was expressed as mg of gallic acid equivalents (GAE) per g of dried extract (de). The TFC of sage extracts (1.75 mg/mL, 1.25 mg/mL and 0.75 mg/mL) was measured by the spectrophotometric method, based on the formation of a flavonoid-aluminium complex. TFC was expressed as mg of quercetin equivalents (QE) per g of dried extract (de). The results were presented as a mean value ± standard deviation (SD) of triplicate measurements. Our findings showed that TPC is in the range from 155 ± 5.55 to 166 ± 2.95 mg GAE/g de. TFC was in range from 31.7 ± 3.61 to 46.6 ± 2.54 mg QE/g de. Compared with published results where extractions of polyphenols from sage were carried out with methanol as an effective and toxic solvent, results obtained in our study demonstrated a high extraction yield of polyphenols from sage using water as a polar and non-toxic solvent.", publisher = "Novi Sad : Faculty of Technical Sciences", journal = "DISC2023 : 3rd DIFENEW International Student Conference : Abstract book", title = "Water as a Green Solvent for the Extraction of Polyphenols from Sage", pages = "12-12", url = "https://hdl.handle.net/21.15107/rcub_vinar_13333" }
Janić, M., Valenta Šobot, A., Momić, T.,& Filipović Tričković, J.. (2023). Water as a Green Solvent for the Extraction of Polyphenols from Sage. in DISC2023 : 3rd DIFENEW International Student Conference : Abstract book Novi Sad : Faculty of Technical Sciences., 12-12. https://hdl.handle.net/21.15107/rcub_vinar_13333
Janić M, Valenta Šobot A, Momić T, Filipović Tričković J. Water as a Green Solvent for the Extraction of Polyphenols from Sage. in DISC2023 : 3rd DIFENEW International Student Conference : Abstract book. 2023;:12-12. https://hdl.handle.net/21.15107/rcub_vinar_13333 .
Janić, Marijana, Valenta Šobot, Ana, Momić, Tatjana, Filipović Tričković, Jelena, "Water as a Green Solvent for the Extraction of Polyphenols from Sage" in DISC2023 : 3rd DIFENEW International Student Conference : Abstract book (2023):12-12, https://hdl.handle.net/21.15107/rcub_vinar_13333 .