Bone microRNA-21 as surgical stress parameter is associated with third molar postoperative discomfort
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2021
Authors
Vučetić, MilanRoganović, Jelena
Freilich, M.
Shafer, D.
Milić, Marija
Đukić, Ljiljana
Petrović, Nina
Marković, Evgenija
Marković, Aleksa
Brković, Božidar
Article (Published version)
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Objective: To evaluate an association between bone levels of inflammation/oxidative stress mediators and postoperative discomfort after third molar conventional or piezosurgery. Material and methods: Twenty-six subjects with bilaterally impacted mandibular third molars, who underwent either piezo or conventional surgery, were included in a split-mouth design study. MicroRNA-21 (miR-21) expression, interleukin-1 beta (IL-1β), and vascular endothelial growth factor (VEGF) proteins, as well as superoxide dismutase (SOD) activity in alveolar bone, were evaluated. Pain intensity, the first pain appearance, analgesic first use and total dose taken, trismus, and swelling were clinically recorded. Results: MiR-21 expression was higher while VEGF protein was lower in piezosurgery vs. conventional groups. The differences in IL-1β protein and SOD activity were not significant between groups. The pain intensity on the first day was significantly decreased in piezosurgery group. The first pain appe...arance and the first analgesic taken were reported sooner in conventional vs. piezosurgical group. Significantly pronounced trismus on the third day following conventional surgery was found. In conventional group, significantly increased trismus was observed on the third compared to the first postoperative day. MiR-21 showed significant correlation with the first pain appearance. Conclusion: Delayed onset of less pronounced postoperative pain after piezosurgical vs. conventional extraction of impacted lower third molar was significantly associated with expression of bone miR-21. Clinical relevance: Alveolar bone miR-21 may reflect surgical stress and is associated with third molar postoperative pain onset.
Keywords:
IL-1β / Lower third molar / miR-21 / Piezo / SOD / VEGFSource:
Clinical Oral Investigations, 2021, 25, 1, 319-328Funding / projects:
- Pain Control and Molecular Mechanisms as Factors for Tissue Regeneration in Dentistry in Healthy and Diabetic Patients (RS-MESTD-Basic Research (BR or ON)-175021)
DOI: 10.1007/s00784-020-03366-6
ISSN: 1432-6981
PubMed: 32495225
WoS: 000538665700001
Scopus: 2-s2.0-85085882257
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VinčaTY - JOUR AU - Vučetić, Milan AU - Roganović, Jelena AU - Freilich, M. AU - Shafer, D. AU - Milić, Marija AU - Đukić, Ljiljana AU - Petrović, Nina AU - Marković, Evgenija AU - Marković, Aleksa AU - Brković, Božidar PY - 2021 UR - https://vinar.vin.bg.ac.rs/handle/123456789/13113 AB - Objective: To evaluate an association between bone levels of inflammation/oxidative stress mediators and postoperative discomfort after third molar conventional or piezosurgery. Material and methods: Twenty-six subjects with bilaterally impacted mandibular third molars, who underwent either piezo or conventional surgery, were included in a split-mouth design study. MicroRNA-21 (miR-21) expression, interleukin-1 beta (IL-1β), and vascular endothelial growth factor (VEGF) proteins, as well as superoxide dismutase (SOD) activity in alveolar bone, were evaluated. Pain intensity, the first pain appearance, analgesic first use and total dose taken, trismus, and swelling were clinically recorded. Results: MiR-21 expression was higher while VEGF protein was lower in piezosurgery vs. conventional groups. The differences in IL-1β protein and SOD activity were not significant between groups. The pain intensity on the first day was significantly decreased in piezosurgery group. The first pain appearance and the first analgesic taken were reported sooner in conventional vs. piezosurgical group. Significantly pronounced trismus on the third day following conventional surgery was found. In conventional group, significantly increased trismus was observed on the third compared to the first postoperative day. MiR-21 showed significant correlation with the first pain appearance. Conclusion: Delayed onset of less pronounced postoperative pain after piezosurgical vs. conventional extraction of impacted lower third molar was significantly associated with expression of bone miR-21. Clinical relevance: Alveolar bone miR-21 may reflect surgical stress and is associated with third molar postoperative pain onset. T2 - Clinical Oral Investigations T1 - Bone microRNA-21 as surgical stress parameter is associated with third molar postoperative discomfort VL - 25 IS - 1 SP - 319 EP - 328 DO - 10.1007/s00784-020-03366-6 ER -
@article{ author = "Vučetić, Milan and Roganović, Jelena and Freilich, M. and Shafer, D. and Milić, Marija and Đukić, Ljiljana and Petrović, Nina and Marković, Evgenija and Marković, Aleksa and Brković, Božidar", year = "2021", abstract = "Objective: To evaluate an association between bone levels of inflammation/oxidative stress mediators and postoperative discomfort after third molar conventional or piezosurgery. Material and methods: Twenty-six subjects with bilaterally impacted mandibular third molars, who underwent either piezo or conventional surgery, were included in a split-mouth design study. MicroRNA-21 (miR-21) expression, interleukin-1 beta (IL-1β), and vascular endothelial growth factor (VEGF) proteins, as well as superoxide dismutase (SOD) activity in alveolar bone, were evaluated. Pain intensity, the first pain appearance, analgesic first use and total dose taken, trismus, and swelling were clinically recorded. Results: MiR-21 expression was higher while VEGF protein was lower in piezosurgery vs. conventional groups. The differences in IL-1β protein and SOD activity were not significant between groups. The pain intensity on the first day was significantly decreased in piezosurgery group. The first pain appearance and the first analgesic taken were reported sooner in conventional vs. piezosurgical group. Significantly pronounced trismus on the third day following conventional surgery was found. In conventional group, significantly increased trismus was observed on the third compared to the first postoperative day. MiR-21 showed significant correlation with the first pain appearance. Conclusion: Delayed onset of less pronounced postoperative pain after piezosurgical vs. conventional extraction of impacted lower third molar was significantly associated with expression of bone miR-21. Clinical relevance: Alveolar bone miR-21 may reflect surgical stress and is associated with third molar postoperative pain onset.", journal = "Clinical Oral Investigations", title = "Bone microRNA-21 as surgical stress parameter is associated with third molar postoperative discomfort", volume = "25", number = "1", pages = "319-328", doi = "10.1007/s00784-020-03366-6" }
Vučetić, M., Roganović, J., Freilich, M., Shafer, D., Milić, M., Đukić, L., Petrović, N., Marković, E., Marković, A.,& Brković, B.. (2021). Bone microRNA-21 as surgical stress parameter is associated with third molar postoperative discomfort. in Clinical Oral Investigations, 25(1), 319-328. https://doi.org/10.1007/s00784-020-03366-6
Vučetić M, Roganović J, Freilich M, Shafer D, Milić M, Đukić L, Petrović N, Marković E, Marković A, Brković B. Bone microRNA-21 as surgical stress parameter is associated with third molar postoperative discomfort. in Clinical Oral Investigations. 2021;25(1):319-328. doi:10.1007/s00784-020-03366-6 .
Vučetić, Milan, Roganović, Jelena, Freilich, M., Shafer, D., Milić, Marija, Đukić, Ljiljana, Petrović, Nina, Marković, Evgenija, Marković, Aleksa, Brković, Božidar, "Bone microRNA-21 as surgical stress parameter is associated with third molar postoperative discomfort" in Clinical Oral Investigations, 25, no. 1 (2021):319-328, https://doi.org/10.1007/s00784-020-03366-6 . .