The effects of gasotransmitters inhibition on homocysteine acutely induced changes in oxidative stress markers in rat plasma
Аутори
Đurić, MarkoKostić, Sanja
Lončar-Stojiljković, Dragana
Mutavdžin, Slavica
Čolović, Mirjana
Krstić, Danijela
Stevanović, Predrag
Đurić, Dragan
Чланак у часопису (Објављена верзија)
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Background: The importance of homocysteine (Hcy) is increasingly recognized in last few decades as an independent risk factor for atherosclerosis and thrombosis, but there is lack of data referring to influence of Hcy on plasma oxidative stress parameters as well as the role of gasotransmitters in these effects. Therefore, this study aim was to assess the role of gasotransmitter inhibitors in Hcy-induced effects on plasma oxidative stress in rats. Material and Methods: Study involved 96 male Wistar albino rats divided into 8 groups: 1) Control group - saline (1ml 0.9 % NaCl i.p.); 2) DL-Hcy (8 mmol/kg i.p. DL homocysteine (DL-Hcy); 3) L-NAME (10 mg/kg i.p. Nω-Nitro-L-arginine methyl ester (L-NAME), inhibitor of NO production); 4) ZnPPR IX (30 mol/kg i.p. protoporphyrin IX zinc (ZnPPR IX), inhibitor of CO production); 5) DL-PAG (50 mg//kg i.p. DL-propargylglycine (DL-PAG), inhibitor of H2S production); 6) DL-Hcy+L-NAME (8 mmol/kg i.p. DL-Hcy + 10 mg/kg i.p. L-NAME); 7) DL-Hcy+ZnPPR IX (...8 mmol/kg i.p. DL-Hcy + 30 mol/kg i.p. Zn PPR IX), and 8) DL-Hcy+DL-PAG (8 mmol/kg i.p. DL-Hcy + 50 mg//kg i.p. DL-PAG). In all experimental groups, tested substances were administered in a single dose, intraperitoneally, 60 minutes before animals' euthanasia. In the collected blood samples malondialdehyde concentration, catalase, glutathione peroxidase and superoxide dismutase activity were measured. Results: Applied substances induced rapid and strong increase of plasma antioxidant enzymatic activity probably as a compensatory response to its pro-oxidant influence. Conclusion: The effects of Hcy on the activity of plasma antioxidant enzymes are in part mediated via interaction with gasotransmitters.
Кључне речи:
gasotransmitters / homocysteine / oxidative stress markers / rat plasmaИзвор:
Scripta Medica, 2019, 50, 1, 6-12Финансирање / пројекти:
- Ефекти хомоцистеина и хомоцистеину сродних супстанци на кардиоваскуларни систем: Улога гасних трансмитера No, H2S и CO (RS-MESTD-Basic Research (BR or ON)-175043)
- COST action [CA16225]
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Институција/група
VinčaTY - JOUR AU - Đurić, Marko AU - Kostić, Sanja AU - Lončar-Stojiljković, Dragana AU - Mutavdžin, Slavica AU - Čolović, Mirjana AU - Krstić, Danijela AU - Stevanović, Predrag AU - Đurić, Dragan PY - 2019 UR - https://vinar.vin.bg.ac.rs/handle/123456789/12973 AB - Background: The importance of homocysteine (Hcy) is increasingly recognized in last few decades as an independent risk factor for atherosclerosis and thrombosis, but there is lack of data referring to influence of Hcy on plasma oxidative stress parameters as well as the role of gasotransmitters in these effects. Therefore, this study aim was to assess the role of gasotransmitter inhibitors in Hcy-induced effects on plasma oxidative stress in rats. Material and Methods: Study involved 96 male Wistar albino rats divided into 8 groups: 1) Control group - saline (1ml 0.9 % NaCl i.p.); 2) DL-Hcy (8 mmol/kg i.p. DL homocysteine (DL-Hcy); 3) L-NAME (10 mg/kg i.p. Nω-Nitro-L-arginine methyl ester (L-NAME), inhibitor of NO production); 4) ZnPPR IX (30 mol/kg i.p. protoporphyrin IX zinc (ZnPPR IX), inhibitor of CO production); 5) DL-PAG (50 mg//kg i.p. DL-propargylglycine (DL-PAG), inhibitor of H2S production); 6) DL-Hcy+L-NAME (8 mmol/kg i.p. DL-Hcy + 10 mg/kg i.p. L-NAME); 7) DL-Hcy+ZnPPR IX (8 mmol/kg i.p. DL-Hcy + 30 mol/kg i.p. Zn PPR IX), and 8) DL-Hcy+DL-PAG (8 mmol/kg i.p. DL-Hcy + 50 mg//kg i.p. DL-PAG). In all experimental groups, tested substances were administered in a single dose, intraperitoneally, 60 minutes before animals' euthanasia. In the collected blood samples malondialdehyde concentration, catalase, glutathione peroxidase and superoxide dismutase activity were measured. Results: Applied substances induced rapid and strong increase of plasma antioxidant enzymatic activity probably as a compensatory response to its pro-oxidant influence. Conclusion: The effects of Hcy on the activity of plasma antioxidant enzymes are in part mediated via interaction with gasotransmitters. T2 - Scripta Medica T1 - The effects of gasotransmitters inhibition on homocysteine acutely induced changes in oxidative stress markers in rat plasma VL - 50 IS - 1 SP - 6 EP - 12 DO - 10.5937/scriptamed50-21100 ER -
@article{ author = "Đurić, Marko and Kostić, Sanja and Lončar-Stojiljković, Dragana and Mutavdžin, Slavica and Čolović, Mirjana and Krstić, Danijela and Stevanović, Predrag and Đurić, Dragan", year = "2019", abstract = "Background: The importance of homocysteine (Hcy) is increasingly recognized in last few decades as an independent risk factor for atherosclerosis and thrombosis, but there is lack of data referring to influence of Hcy on plasma oxidative stress parameters as well as the role of gasotransmitters in these effects. Therefore, this study aim was to assess the role of gasotransmitter inhibitors in Hcy-induced effects on plasma oxidative stress in rats. Material and Methods: Study involved 96 male Wistar albino rats divided into 8 groups: 1) Control group - saline (1ml 0.9 % NaCl i.p.); 2) DL-Hcy (8 mmol/kg i.p. DL homocysteine (DL-Hcy); 3) L-NAME (10 mg/kg i.p. Nω-Nitro-L-arginine methyl ester (L-NAME), inhibitor of NO production); 4) ZnPPR IX (30 mol/kg i.p. protoporphyrin IX zinc (ZnPPR IX), inhibitor of CO production); 5) DL-PAG (50 mg//kg i.p. DL-propargylglycine (DL-PAG), inhibitor of H2S production); 6) DL-Hcy+L-NAME (8 mmol/kg i.p. DL-Hcy + 10 mg/kg i.p. L-NAME); 7) DL-Hcy+ZnPPR IX (8 mmol/kg i.p. DL-Hcy + 30 mol/kg i.p. Zn PPR IX), and 8) DL-Hcy+DL-PAG (8 mmol/kg i.p. DL-Hcy + 50 mg//kg i.p. DL-PAG). In all experimental groups, tested substances were administered in a single dose, intraperitoneally, 60 minutes before animals' euthanasia. In the collected blood samples malondialdehyde concentration, catalase, glutathione peroxidase and superoxide dismutase activity were measured. Results: Applied substances induced rapid and strong increase of plasma antioxidant enzymatic activity probably as a compensatory response to its pro-oxidant influence. Conclusion: The effects of Hcy on the activity of plasma antioxidant enzymes are in part mediated via interaction with gasotransmitters.", journal = "Scripta Medica", title = "The effects of gasotransmitters inhibition on homocysteine acutely induced changes in oxidative stress markers in rat plasma", volume = "50", number = "1", pages = "6-12", doi = "10.5937/scriptamed50-21100" }
Đurić, M., Kostić, S., Lončar-Stojiljković, D., Mutavdžin, S., Čolović, M., Krstić, D., Stevanović, P.,& Đurić, D.. (2019). The effects of gasotransmitters inhibition on homocysteine acutely induced changes in oxidative stress markers in rat plasma. in Scripta Medica, 50(1), 6-12. https://doi.org/10.5937/scriptamed50-21100
Đurić M, Kostić S, Lončar-Stojiljković D, Mutavdžin S, Čolović M, Krstić D, Stevanović P, Đurić D. The effects of gasotransmitters inhibition on homocysteine acutely induced changes in oxidative stress markers in rat plasma. in Scripta Medica. 2019;50(1):6-12. doi:10.5937/scriptamed50-21100 .
Đurić, Marko, Kostić, Sanja, Lončar-Stojiljković, Dragana, Mutavdžin, Slavica, Čolović, Mirjana, Krstić, Danijela, Stevanović, Predrag, Đurić, Dragan, "The effects of gasotransmitters inhibition on homocysteine acutely induced changes in oxidative stress markers in rat plasma" in Scripta Medica, 50, no. 1 (2019):6-12, https://doi.org/10.5937/scriptamed50-21100 . .