Biological activity and binding properties of [Ru(II)(dcbpy)(2)Cl-2] complex to bovine serum albumin, phospholipase A(2) and glutathione
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2016
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Ruthenium compounds are highly regarded as metallo-drug candidates. Many studies have focused their attention on the interaction between ruthenium complexes with their possible biological targets. The interaction of ruthenium complexes with transport proteins, enzymes and peptides is of great importance for understanding their biodistribution and mechanism of action, therefore, the development of an anti-cancer therapy involving ruthenium complexes has recently shifted from DNA targeting towards protein targeting. With the aim of gaining insight into possible interactions between ruthenium complexes with biologically relevant proteins, we have studied the interaction of cis-dichlorobis(2,2-bipyridyl-4,4-dicarboxylic acid)ruthenium(II) complex [Ru(II)(dcbpy)(2)Cl-2], which previously showed good potency in photo-dynamic chemotherapy, with bovine serum albumin (BSA), phospholipase A(2) (PLA(2)) and glutathione (GSH). Binding constants and possible number of binding sites to mentioned pro...teins and peptide are investigated by ultraviolet-visible spectroscopy and Matrix-Assisted Laser Desorption Ionization Mass Spectrometry (MALDI TOF MS). The complex binding affinities were in the following order: PLA(2) GT BSA GT GSH. Moreover, genotoxic profile of the complex, tested on peripheral blood lymphocytes as a model system, was also promising.
Ključne reči:
Ruthenium (II) complex / Potential metallo-drug / Biological targets / Proteins / GlutathioneIzvor:
Biometals, 2016, 29, 5, 921-933Finansiranje / projekti:
- Istraživanja interakcija enzima sa toksičnim i farmakološki aktivnim molekulima (RS-MESTD-Basic Research (BR or ON)-172023)
- Ispitivanje mehanizma reakcija kompleksa jona prelaznih metala sa biološki značajnim molekulima (RS-MESTD-Basic Research (BR or ON)-172011)
DOI: 10.1007/s10534-016-9964-y
ISSN: 0966-0844; 1572-8773
PubMed: 27515969
WoS: 000385252400015
Scopus: 2-s2.0-84981486939
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Institucija/grupa
VinčaTY - JOUR AU - Nešić, Maja D. AU - Popović, Iva A. AU - Leskovac, Andreja AU - Petković, Marijana PY - 2016 UR - https://vinar.vin.bg.ac.rs/handle/123456789/1271 AB - Ruthenium compounds are highly regarded as metallo-drug candidates. Many studies have focused their attention on the interaction between ruthenium complexes with their possible biological targets. The interaction of ruthenium complexes with transport proteins, enzymes and peptides is of great importance for understanding their biodistribution and mechanism of action, therefore, the development of an anti-cancer therapy involving ruthenium complexes has recently shifted from DNA targeting towards protein targeting. With the aim of gaining insight into possible interactions between ruthenium complexes with biologically relevant proteins, we have studied the interaction of cis-dichlorobis(2,2-bipyridyl-4,4-dicarboxylic acid)ruthenium(II) complex [Ru(II)(dcbpy)(2)Cl-2], which previously showed good potency in photo-dynamic chemotherapy, with bovine serum albumin (BSA), phospholipase A(2) (PLA(2)) and glutathione (GSH). Binding constants and possible number of binding sites to mentioned proteins and peptide are investigated by ultraviolet-visible spectroscopy and Matrix-Assisted Laser Desorption Ionization Mass Spectrometry (MALDI TOF MS). The complex binding affinities were in the following order: PLA(2) GT BSA GT GSH. Moreover, genotoxic profile of the complex, tested on peripheral blood lymphocytes as a model system, was also promising. T2 - Biometals T1 - Biological activity and binding properties of [Ru(II)(dcbpy)(2)Cl-2] complex to bovine serum albumin, phospholipase A(2) and glutathione VL - 29 IS - 5 SP - 921 EP - 933 DO - 10.1007/s10534-016-9964-y ER -
@article{ author = "Nešić, Maja D. and Popović, Iva A. and Leskovac, Andreja and Petković, Marijana", year = "2016", abstract = "Ruthenium compounds are highly regarded as metallo-drug candidates. Many studies have focused their attention on the interaction between ruthenium complexes with their possible biological targets. The interaction of ruthenium complexes with transport proteins, enzymes and peptides is of great importance for understanding their biodistribution and mechanism of action, therefore, the development of an anti-cancer therapy involving ruthenium complexes has recently shifted from DNA targeting towards protein targeting. With the aim of gaining insight into possible interactions between ruthenium complexes with biologically relevant proteins, we have studied the interaction of cis-dichlorobis(2,2-bipyridyl-4,4-dicarboxylic acid)ruthenium(II) complex [Ru(II)(dcbpy)(2)Cl-2], which previously showed good potency in photo-dynamic chemotherapy, with bovine serum albumin (BSA), phospholipase A(2) (PLA(2)) and glutathione (GSH). Binding constants and possible number of binding sites to mentioned proteins and peptide are investigated by ultraviolet-visible spectroscopy and Matrix-Assisted Laser Desorption Ionization Mass Spectrometry (MALDI TOF MS). The complex binding affinities were in the following order: PLA(2) GT BSA GT GSH. Moreover, genotoxic profile of the complex, tested on peripheral blood lymphocytes as a model system, was also promising.", journal = "Biometals", title = "Biological activity and binding properties of [Ru(II)(dcbpy)(2)Cl-2] complex to bovine serum albumin, phospholipase A(2) and glutathione", volume = "29", number = "5", pages = "921-933", doi = "10.1007/s10534-016-9964-y" }
Nešić, M. D., Popović, I. A., Leskovac, A.,& Petković, M.. (2016). Biological activity and binding properties of [Ru(II)(dcbpy)(2)Cl-2] complex to bovine serum albumin, phospholipase A(2) and glutathione. in Biometals, 29(5), 921-933. https://doi.org/10.1007/s10534-016-9964-y
Nešić MD, Popović IA, Leskovac A, Petković M. Biological activity and binding properties of [Ru(II)(dcbpy)(2)Cl-2] complex to bovine serum albumin, phospholipase A(2) and glutathione. in Biometals. 2016;29(5):921-933. doi:10.1007/s10534-016-9964-y .
Nešić, Maja D., Popović, Iva A., Leskovac, Andreja, Petković, Marijana, "Biological activity and binding properties of [Ru(II)(dcbpy)(2)Cl-2] complex to bovine serum albumin, phospholipase A(2) and glutathione" in Biometals, 29, no. 5 (2016):921-933, https://doi.org/10.1007/s10534-016-9964-y . .