Deep UV fluorescence imaging study of Candida albicans cells treated with gold-riboflavin hydrocolloids
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2016
Authors
Pajović, Jelena D.Dojčilović, Radovan
Božanić, Dušan K.
Vodnik, Vesna
Dimitrijević-Branković, Suzana I.
Kaščakova, Slavka
Refregiers, Matthieu
Markelić, Milica
Đoković, Vladimir
Article (Published version)
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Show full item recordAbstract
Fluorescent nanostructures were prepared by functionalization of gold nanoparticles with riboflavin molecules and used as probes for synchrotron radiation deep ultraviolet (SR-DUV) fluorescence imaging of gluteraldehyde-fixed Candida albicans cells. The nanoparticles were characterized by transmission electron microscopy (TEM) and optical spectroscopy techniques. The TEM analysis showed that the nanostructures were 6 nm in diameter, while the results of the fluorescence spectroscopies confirmed the photoluminescence of the nanoparticles. The SR-DUV imaging showed that it was possible to distinguish the fluorescence of the nanoparticles from the autofluorescence of the cells, as well as that the local maxima of the signal pertaining to the fluorescence of goldriboflavin nanostructures were mostly positioned on the surfaces of the C. albicans cells of spherical morphology.
Keywords:
Gold nanoparticles / Riboflavin / Functionalization / Candida albicans / Fluorescence imagingSource:
Optical and Quantum Electronics, 2016, 48, 6Funding / projects:
- Materials of Reduced Dimensions for Efficient Light Harvesting and Energy conversion (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-45020)
- Size-, shape- and structure- dependent properties of nanoparticles and nanocomposites (RS-MESTD-Basic Research (BR or ON)-172056)
- Investigation of the effect of parameters of synthesis and processing on dielectric, optical and magnetic properties, both bulk and surface of crystal and polymeric systems (RS-MESTD-Basic Research (BR or ON)-171029)
- [20120810], [20131218]
DOI: 10.1007/s11082-016-0578-y
ISSN: 0306-8919; 1572-817X
WoS: 000377387300004
Scopus: 2-s2.0-84966440446
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VinčaTY - JOUR AU - Pajović, Jelena D. AU - Dojčilović, Radovan AU - Božanić, Dušan K. AU - Vodnik, Vesna AU - Dimitrijević-Branković, Suzana I. AU - Kaščakova, Slavka AU - Refregiers, Matthieu AU - Markelić, Milica AU - Đoković, Vladimir PY - 2016 UR - https://vinar.vin.bg.ac.rs/handle/123456789/1107 AB - Fluorescent nanostructures were prepared by functionalization of gold nanoparticles with riboflavin molecules and used as probes for synchrotron radiation deep ultraviolet (SR-DUV) fluorescence imaging of gluteraldehyde-fixed Candida albicans cells. The nanoparticles were characterized by transmission electron microscopy (TEM) and optical spectroscopy techniques. The TEM analysis showed that the nanostructures were 6 nm in diameter, while the results of the fluorescence spectroscopies confirmed the photoluminescence of the nanoparticles. The SR-DUV imaging showed that it was possible to distinguish the fluorescence of the nanoparticles from the autofluorescence of the cells, as well as that the local maxima of the signal pertaining to the fluorescence of goldriboflavin nanostructures were mostly positioned on the surfaces of the C. albicans cells of spherical morphology. T2 - Optical and Quantum Electronics T1 - Deep UV fluorescence imaging study of Candida albicans cells treated with gold-riboflavin hydrocolloids VL - 48 IS - 6 DO - 10.1007/s11082-016-0578-y ER -
@article{ author = "Pajović, Jelena D. and Dojčilović, Radovan and Božanić, Dušan K. and Vodnik, Vesna and Dimitrijević-Branković, Suzana I. and Kaščakova, Slavka and Refregiers, Matthieu and Markelić, Milica and Đoković, Vladimir", year = "2016", abstract = "Fluorescent nanostructures were prepared by functionalization of gold nanoparticles with riboflavin molecules and used as probes for synchrotron radiation deep ultraviolet (SR-DUV) fluorescence imaging of gluteraldehyde-fixed Candida albicans cells. The nanoparticles were characterized by transmission electron microscopy (TEM) and optical spectroscopy techniques. The TEM analysis showed that the nanostructures were 6 nm in diameter, while the results of the fluorescence spectroscopies confirmed the photoluminescence of the nanoparticles. The SR-DUV imaging showed that it was possible to distinguish the fluorescence of the nanoparticles from the autofluorescence of the cells, as well as that the local maxima of the signal pertaining to the fluorescence of goldriboflavin nanostructures were mostly positioned on the surfaces of the C. albicans cells of spherical morphology.", journal = "Optical and Quantum Electronics", title = "Deep UV fluorescence imaging study of Candida albicans cells treated with gold-riboflavin hydrocolloids", volume = "48", number = "6", doi = "10.1007/s11082-016-0578-y" }
Pajović, J. D., Dojčilović, R., Božanić, D. K., Vodnik, V., Dimitrijević-Branković, S. I., Kaščakova, S., Refregiers, M., Markelić, M.,& Đoković, V.. (2016). Deep UV fluorescence imaging study of Candida albicans cells treated with gold-riboflavin hydrocolloids. in Optical and Quantum Electronics, 48(6). https://doi.org/10.1007/s11082-016-0578-y
Pajović JD, Dojčilović R, Božanić DK, Vodnik V, Dimitrijević-Branković SI, Kaščakova S, Refregiers M, Markelić M, Đoković V. Deep UV fluorescence imaging study of Candida albicans cells treated with gold-riboflavin hydrocolloids. in Optical and Quantum Electronics. 2016;48(6). doi:10.1007/s11082-016-0578-y .
Pajović, Jelena D., Dojčilović, Radovan, Božanić, Dušan K., Vodnik, Vesna, Dimitrijević-Branković, Suzana I., Kaščakova, Slavka, Refregiers, Matthieu, Markelić, Milica, Đoković, Vladimir, "Deep UV fluorescence imaging study of Candida albicans cells treated with gold-riboflavin hydrocolloids" in Optical and Quantum Electronics, 48, no. 6 (2016), https://doi.org/10.1007/s11082-016-0578-y . .