Subacute Exposure to Low Pb Doses Promotes Oxidative Stress in the Kidneys and Copper Disturbances in the Liver of Male Rats
Аутори
Vukelić, DraganaBuha-Đorđević, Aleksandra
Anđelković, Milena
Antonijević Miljaković, Evica
Baralić, Katarina
Živančević, Katarina
Bulat, Petar
Radovanović, Jelena
Đukić-Ćosić, Danijela
Antonijević, Biljana
Bulat, Zorica
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Recent data indicate that lead (Pb) can induce adverse effects even at low exposure levels. Moreover, the corresponding mechanisms of low Pb toxicity have not been well identified. In the liver and the kidneys, Pb was found to induce various toxic mechanisms leading to organ physiological disruption. Therefore, the purpose of the study was to simulate low-dose Pb exposure in an animal model with the aim of assessing oxidative status and essential element levels as the main mechanism of Pb toxicity in the liver and kidneys. Furthermore, dose–response modelling was performed in order to determine the benchmark dose (BMD). Forty-two male Wistar rats were divided into seven groups: one control group, and six groups treated for 28 days with 0.1, 0.5, 1, 3, 7, and 15 mg Pb/kg b.w./day, respectively. Oxidative status parameters (superoxide dismutase activity (SOD), superoxide anion radical (O2−), malondialdehyde (MDA), total sulfhydryl groups (SHG), and advanced oxidation protein products (AO...PP)) and Pb, copper (Cu), zinc (Zn), manganese (Mn), and iron (Fe) levels were measured. Lowering Cu levels (BMD: 2.7 ng/kg b.w./day), raising AOPP levels (BMD: 0.25 µg/kg b.w./day) in the liver, and inhibiting SOD (BMD: 1.3 ng/kg b.w./day) in the kidneys appear to be the main mechanisms of Pb toxicity. The lowest BMD was derived for a decrease in Cu levels in liver, indicating that this effect is the most sensitive.
Кључне речи:
benchmark modelling / dose–response / essential elements / oxidative stress / Pb exposureИзвор:
Toxics, 2023, 11, 3, 256-Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200161 (Универзитет у Београду, Фармацеутски факултет) (RS-MESTD-inst-2020-200161)
Институција/група
VinčaTY - JOUR AU - Vukelić, Dragana AU - Buha-Đorđević, Aleksandra AU - Anđelković, Milena AU - Antonijević Miljaković, Evica AU - Baralić, Katarina AU - Živančević, Katarina AU - Bulat, Petar AU - Radovanović, Jelena AU - Đukić-Ćosić, Danijela AU - Antonijević, Biljana AU - Bulat, Zorica PY - 2023 UR - https://vinar.vin.bg.ac.rs/handle/123456789/10809 AB - Recent data indicate that lead (Pb) can induce adverse effects even at low exposure levels. Moreover, the corresponding mechanisms of low Pb toxicity have not been well identified. In the liver and the kidneys, Pb was found to induce various toxic mechanisms leading to organ physiological disruption. Therefore, the purpose of the study was to simulate low-dose Pb exposure in an animal model with the aim of assessing oxidative status and essential element levels as the main mechanism of Pb toxicity in the liver and kidneys. Furthermore, dose–response modelling was performed in order to determine the benchmark dose (BMD). Forty-two male Wistar rats were divided into seven groups: one control group, and six groups treated for 28 days with 0.1, 0.5, 1, 3, 7, and 15 mg Pb/kg b.w./day, respectively. Oxidative status parameters (superoxide dismutase activity (SOD), superoxide anion radical (O2−), malondialdehyde (MDA), total sulfhydryl groups (SHG), and advanced oxidation protein products (AOPP)) and Pb, copper (Cu), zinc (Zn), manganese (Mn), and iron (Fe) levels were measured. Lowering Cu levels (BMD: 2.7 ng/kg b.w./day), raising AOPP levels (BMD: 0.25 µg/kg b.w./day) in the liver, and inhibiting SOD (BMD: 1.3 ng/kg b.w./day) in the kidneys appear to be the main mechanisms of Pb toxicity. The lowest BMD was derived for a decrease in Cu levels in liver, indicating that this effect is the most sensitive. T2 - Toxics T1 - Subacute Exposure to Low Pb Doses Promotes Oxidative Stress in the Kidneys and Copper Disturbances in the Liver of Male Rats VL - 11 IS - 3 SP - 256 DO - 10.3390/toxics11030256 ER -
@article{ author = "Vukelić, Dragana and Buha-Đorđević, Aleksandra and Anđelković, Milena and Antonijević Miljaković, Evica and Baralić, Katarina and Živančević, Katarina and Bulat, Petar and Radovanović, Jelena and Đukić-Ćosić, Danijela and Antonijević, Biljana and Bulat, Zorica", year = "2023", abstract = "Recent data indicate that lead (Pb) can induce adverse effects even at low exposure levels. Moreover, the corresponding mechanisms of low Pb toxicity have not been well identified. In the liver and the kidneys, Pb was found to induce various toxic mechanisms leading to organ physiological disruption. Therefore, the purpose of the study was to simulate low-dose Pb exposure in an animal model with the aim of assessing oxidative status and essential element levels as the main mechanism of Pb toxicity in the liver and kidneys. Furthermore, dose–response modelling was performed in order to determine the benchmark dose (BMD). Forty-two male Wistar rats were divided into seven groups: one control group, and six groups treated for 28 days with 0.1, 0.5, 1, 3, 7, and 15 mg Pb/kg b.w./day, respectively. Oxidative status parameters (superoxide dismutase activity (SOD), superoxide anion radical (O2−), malondialdehyde (MDA), total sulfhydryl groups (SHG), and advanced oxidation protein products (AOPP)) and Pb, copper (Cu), zinc (Zn), manganese (Mn), and iron (Fe) levels were measured. Lowering Cu levels (BMD: 2.7 ng/kg b.w./day), raising AOPP levels (BMD: 0.25 µg/kg b.w./day) in the liver, and inhibiting SOD (BMD: 1.3 ng/kg b.w./day) in the kidneys appear to be the main mechanisms of Pb toxicity. The lowest BMD was derived for a decrease in Cu levels in liver, indicating that this effect is the most sensitive.", journal = "Toxics", title = "Subacute Exposure to Low Pb Doses Promotes Oxidative Stress in the Kidneys and Copper Disturbances in the Liver of Male Rats", volume = "11", number = "3", pages = "256", doi = "10.3390/toxics11030256" }
Vukelić, D., Buha-Đorđević, A., Anđelković, M., Antonijević Miljaković, E., Baralić, K., Živančević, K., Bulat, P., Radovanović, J., Đukić-Ćosić, D., Antonijević, B.,& Bulat, Z.. (2023). Subacute Exposure to Low Pb Doses Promotes Oxidative Stress in the Kidneys and Copper Disturbances in the Liver of Male Rats. in Toxics, 11(3), 256. https://doi.org/10.3390/toxics11030256
Vukelić D, Buha-Đorđević A, Anđelković M, Antonijević Miljaković E, Baralić K, Živančević K, Bulat P, Radovanović J, Đukić-Ćosić D, Antonijević B, Bulat Z. Subacute Exposure to Low Pb Doses Promotes Oxidative Stress in the Kidneys and Copper Disturbances in the Liver of Male Rats. in Toxics. 2023;11(3):256. doi:10.3390/toxics11030256 .
Vukelić, Dragana, Buha-Đorđević, Aleksandra, Anđelković, Milena, Antonijević Miljaković, Evica, Baralić, Katarina, Živančević, Katarina, Bulat, Petar, Radovanović, Jelena, Đukić-Ćosić, Danijela, Antonijević, Biljana, Bulat, Zorica, "Subacute Exposure to Low Pb Doses Promotes Oxidative Stress in the Kidneys and Copper Disturbances in the Liver of Male Rats" in Toxics, 11, no. 3 (2023):256, https://doi.org/10.3390/toxics11030256 . .