Superoxide dismutases and p53 protein levels in blood cells of breast cancer patients
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Purpose: Radiation treatment of breast cancer (BC) often results in post-therapy complications. The undesired sequelae could be avoided by the diagnostic screening of biomarkers for prediction of ionizing radiation (IR)-linked injury of healthy tissues. Patients and methods: The expression of antioxidative defence enzymes CuZn- and Mn-superoxide dismutase (CuZnSOD, MnSOD) and tumor suppressor protein p53 was measured in blood cells of 19 women with BC (age groups 30-45 and 46-60 years) and of the respective controls. The proteins were detected by specific immunostaining and quantified by laser-scanning densitometry. Results: Constitutive expression of CuZnSOD was significantly elevated in the group of BC patients (up to 254 arbitrary units, AU/mL) relative to the control group (105-130 AU/mL). The constitutive expression of MnSOD was elevated (up to 94 AU/mL) in the group of BC patients relative to the controls (53-56 AU/mL). p53 was also constitutively more expressed in BC patients (3...5-42 AU/mL) than in controls (32-33 AU/mL). Both MnSOD and p53 were inducible by 60Co gamma-ray IR (up to 170 AU/mL and 51 AU/mL, respectively) in the BC patient group. The levels of IR-induced p53 correlated inversely with MnSOD levels. Conclusion: The constitutive expression of all 3 proteins could be a useful biomarker for the presence of BC, but only MnSOD overexpression may be the predictive biomarker for selection of BC patients that would be less susceptible to IR-linked complications. © 2004 Zerbinis Medical Publications.
Кључне речи:
Blood cells / Breast cancer / Ionizing radiation / p53 / Superoxide dismutasesИзвор:
Journal of Balkan Union of Oncology., 2004, 9, 3, 283-287Финансирање / пројекти:
- Ministry of Sciences and Environment Protection of the Republic of Serbia [Grant BOI1953]
Институција/група
VinčaTY - JOUR AU - Adžić, Miroslav AU - Nićiforović, Ana AU - Nešković-Konstantinović, Zora AU - Radojčić, Marija B. PY - 2004 UR - https://vinar.vin.bg.ac.rs/handle/123456789/10582 AB - Purpose: Radiation treatment of breast cancer (BC) often results in post-therapy complications. The undesired sequelae could be avoided by the diagnostic screening of biomarkers for prediction of ionizing radiation (IR)-linked injury of healthy tissues. Patients and methods: The expression of antioxidative defence enzymes CuZn- and Mn-superoxide dismutase (CuZnSOD, MnSOD) and tumor suppressor protein p53 was measured in blood cells of 19 women with BC (age groups 30-45 and 46-60 years) and of the respective controls. The proteins were detected by specific immunostaining and quantified by laser-scanning densitometry. Results: Constitutive expression of CuZnSOD was significantly elevated in the group of BC patients (up to 254 arbitrary units, AU/mL) relative to the control group (105-130 AU/mL). The constitutive expression of MnSOD was elevated (up to 94 AU/mL) in the group of BC patients relative to the controls (53-56 AU/mL). p53 was also constitutively more expressed in BC patients (35-42 AU/mL) than in controls (32-33 AU/mL). Both MnSOD and p53 were inducible by 60Co gamma-ray IR (up to 170 AU/mL and 51 AU/mL, respectively) in the BC patient group. The levels of IR-induced p53 correlated inversely with MnSOD levels. Conclusion: The constitutive expression of all 3 proteins could be a useful biomarker for the presence of BC, but only MnSOD overexpression may be the predictive biomarker for selection of BC patients that would be less susceptible to IR-linked complications. © 2004 Zerbinis Medical Publications. T2 - Journal of Balkan Union of Oncology. T1 - Superoxide dismutases and p53 protein levels in blood cells of breast cancer patients VL - 9 IS - 3 SP - 283 EP - 287 UR - https://hdl.handle.net/21.15107/rcub_vinar_10582 ER -
@article{ author = "Adžić, Miroslav and Nićiforović, Ana and Nešković-Konstantinović, Zora and Radojčić, Marija B.", year = "2004", abstract = "Purpose: Radiation treatment of breast cancer (BC) often results in post-therapy complications. The undesired sequelae could be avoided by the diagnostic screening of biomarkers for prediction of ionizing radiation (IR)-linked injury of healthy tissues. Patients and methods: The expression of antioxidative defence enzymes CuZn- and Mn-superoxide dismutase (CuZnSOD, MnSOD) and tumor suppressor protein p53 was measured in blood cells of 19 women with BC (age groups 30-45 and 46-60 years) and of the respective controls. The proteins were detected by specific immunostaining and quantified by laser-scanning densitometry. Results: Constitutive expression of CuZnSOD was significantly elevated in the group of BC patients (up to 254 arbitrary units, AU/mL) relative to the control group (105-130 AU/mL). The constitutive expression of MnSOD was elevated (up to 94 AU/mL) in the group of BC patients relative to the controls (53-56 AU/mL). p53 was also constitutively more expressed in BC patients (35-42 AU/mL) than in controls (32-33 AU/mL). Both MnSOD and p53 were inducible by 60Co gamma-ray IR (up to 170 AU/mL and 51 AU/mL, respectively) in the BC patient group. The levels of IR-induced p53 correlated inversely with MnSOD levels. Conclusion: The constitutive expression of all 3 proteins could be a useful biomarker for the presence of BC, but only MnSOD overexpression may be the predictive biomarker for selection of BC patients that would be less susceptible to IR-linked complications. © 2004 Zerbinis Medical Publications.", journal = "Journal of Balkan Union of Oncology.", title = "Superoxide dismutases and p53 protein levels in blood cells of breast cancer patients", volume = "9", number = "3", pages = "283-287", url = "https://hdl.handle.net/21.15107/rcub_vinar_10582" }
Adžić, M., Nićiforović, A., Nešković-Konstantinović, Z.,& Radojčić, M. B.. (2004). Superoxide dismutases and p53 protein levels in blood cells of breast cancer patients. in Journal of Balkan Union of Oncology., 9(3), 283-287. https://hdl.handle.net/21.15107/rcub_vinar_10582
Adžić M, Nićiforović A, Nešković-Konstantinović Z, Radojčić MB. Superoxide dismutases and p53 protein levels in blood cells of breast cancer patients. in Journal of Balkan Union of Oncology.. 2004;9(3):283-287. https://hdl.handle.net/21.15107/rcub_vinar_10582 .
Adžić, Miroslav, Nićiforović, Ana, Nešković-Konstantinović, Zora, Radojčić, Marija B., "Superoxide dismutases and p53 protein levels in blood cells of breast cancer patients" in Journal of Balkan Union of Oncology., 9, no. 3 (2004):283-287, https://hdl.handle.net/21.15107/rcub_vinar_10582 .