Biochemical changes in cancer cells induced by photoactive nanosystem based on carbon dots loaded with Ru-complex
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2022
Authors
Nešić, Maja D.Dučić, Tanja
Gonçalves, Mara
Stepić, Milutin
Algarra, Manuel
Soto, Juan
Gemović, Branislava S.
Bandosz, Teresa J.
Petković, Marijana
Article (Published version)
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Carbon dots (CDs) and N-carbon dots (N-CDs) loaded with Ru-complex (CDs@RuCN, N-CDs@RuCN, respectively) were investigated as media imposing biochemical changes induced by UV illumination of ovarian cancer, A2780, and osteosarcoma, CAL72, cells. Synchrotron radiation-based Fourier Transform Infrared Spectroscopy was performed, and the spectra were subjected to a Principal Component Analysis. The CDs@RuCN and N-CDs@RuCN effects on cancer cells were analyzed by the theoretical modelling of the stability of the composite systems and a protein database search. Moreover, a detailed evaluation of surface and optical properties of CDs@RuCN and N-CDs@RuCN was carried out. Results demonstrated selective action of the CDs@RuCN and N-CDs@RuCN-based photodynamic therapy, with N-CDs@RuCN being the most active in inducing changes in A2780 and CDs@RuCN in CAL72 cells. We assume that different surface charges of nanoparticles led to direct interactions of N-CDs@RuCN with a Wnt signalling pathway in A27...80 and those of CDs@RuCN with PI3–K/Akt in CAL72 cells and that further biochemical changes occurred upon light illumination.
Keywords:
Anticancer photodynamic therapy / Biochemical changes / Carbon dots / N-doped carbon dots / Ru-metallodrugSource:
Chemico-Biological Interactions, 2022, 360, 109950-Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200017 (University of Belgrade, Institute of Nuclear Sciences 'Vinča', Belgrade-Vinča) (RS-MESTD-inst-2020-200017)
- Programmatic Fund - [UIDP/00674/2020]
- FCT- (CQM Base Fund - [UIDB/00674/2020]
- Madeira 14–20 [PRO- EQUIPRAM-Reforço do Investimentoem Equipamentos e Infrastructures Científcasna RAM-M1420-01-0145-FEDER-000008]
- Madeira 14–20 [M1420-01-0145-FEDER-000005-CQM+]
- ARDITI [ARDITI-CQM/2018/007-PDG]
- Innovaci ́on y Universidades, Spain [(RTI2018-099668-BC2]
- FEDER [UMA18-FEDER-JA-049]
- FEDER [P18- RT-4592]
DOI: 10.1016/j.cbi.2022.109950
ISSN: 0009-2797
WoS: 00079357400000
Scopus: 2-s2.0-85129907279
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VinčaTY - JOUR AU - Nešić, Maja D. AU - Dučić, Tanja AU - Gonçalves, Mara AU - Stepić, Milutin AU - Algarra, Manuel AU - Soto, Juan AU - Gemović, Branislava S. AU - Bandosz, Teresa J. AU - Petković, Marijana PY - 2022 UR - https://vinar.vin.bg.ac.rs/handle/123456789/10267 AB - Carbon dots (CDs) and N-carbon dots (N-CDs) loaded with Ru-complex (CDs@RuCN, N-CDs@RuCN, respectively) were investigated as media imposing biochemical changes induced by UV illumination of ovarian cancer, A2780, and osteosarcoma, CAL72, cells. Synchrotron radiation-based Fourier Transform Infrared Spectroscopy was performed, and the spectra were subjected to a Principal Component Analysis. The CDs@RuCN and N-CDs@RuCN effects on cancer cells were analyzed by the theoretical modelling of the stability of the composite systems and a protein database search. Moreover, a detailed evaluation of surface and optical properties of CDs@RuCN and N-CDs@RuCN was carried out. Results demonstrated selective action of the CDs@RuCN and N-CDs@RuCN-based photodynamic therapy, with N-CDs@RuCN being the most active in inducing changes in A2780 and CDs@RuCN in CAL72 cells. We assume that different surface charges of nanoparticles led to direct interactions of N-CDs@RuCN with a Wnt signalling pathway in A2780 and those of CDs@RuCN with PI3–K/Akt in CAL72 cells and that further biochemical changes occurred upon light illumination. T2 - Chemico-Biological Interactions T1 - Biochemical changes in cancer cells induced by photoactive nanosystem based on carbon dots loaded with Ru-complex VL - 360 SP - 109950 DO - 10.1016/j.cbi.2022.109950 ER -
@article{ author = "Nešić, Maja D. and Dučić, Tanja and Gonçalves, Mara and Stepić, Milutin and Algarra, Manuel and Soto, Juan and Gemović, Branislava S. and Bandosz, Teresa J. and Petković, Marijana", year = "2022", abstract = "Carbon dots (CDs) and N-carbon dots (N-CDs) loaded with Ru-complex (CDs@RuCN, N-CDs@RuCN, respectively) were investigated as media imposing biochemical changes induced by UV illumination of ovarian cancer, A2780, and osteosarcoma, CAL72, cells. Synchrotron radiation-based Fourier Transform Infrared Spectroscopy was performed, and the spectra were subjected to a Principal Component Analysis. The CDs@RuCN and N-CDs@RuCN effects on cancer cells were analyzed by the theoretical modelling of the stability of the composite systems and a protein database search. Moreover, a detailed evaluation of surface and optical properties of CDs@RuCN and N-CDs@RuCN was carried out. Results demonstrated selective action of the CDs@RuCN and N-CDs@RuCN-based photodynamic therapy, with N-CDs@RuCN being the most active in inducing changes in A2780 and CDs@RuCN in CAL72 cells. We assume that different surface charges of nanoparticles led to direct interactions of N-CDs@RuCN with a Wnt signalling pathway in A2780 and those of CDs@RuCN with PI3–K/Akt in CAL72 cells and that further biochemical changes occurred upon light illumination.", journal = "Chemico-Biological Interactions", title = "Biochemical changes in cancer cells induced by photoactive nanosystem based on carbon dots loaded with Ru-complex", volume = "360", pages = "109950", doi = "10.1016/j.cbi.2022.109950" }
Nešić, M. D., Dučić, T., Gonçalves, M., Stepić, M., Algarra, M., Soto, J., Gemović, B. S., Bandosz, T. J.,& Petković, M.. (2022). Biochemical changes in cancer cells induced by photoactive nanosystem based on carbon dots loaded with Ru-complex. in Chemico-Biological Interactions, 360, 109950. https://doi.org/10.1016/j.cbi.2022.109950
Nešić MD, Dučić T, Gonçalves M, Stepić M, Algarra M, Soto J, Gemović BS, Bandosz TJ, Petković M. Biochemical changes in cancer cells induced by photoactive nanosystem based on carbon dots loaded with Ru-complex. in Chemico-Biological Interactions. 2022;360:109950. doi:10.1016/j.cbi.2022.109950 .
Nešić, Maja D., Dučić, Tanja, Gonçalves, Mara, Stepić, Milutin, Algarra, Manuel, Soto, Juan, Gemović, Branislava S., Bandosz, Teresa J., Petković, Marijana, "Biochemical changes in cancer cells induced by photoactive nanosystem based on carbon dots loaded with Ru-complex" in Chemico-Biological Interactions, 360 (2022):109950, https://doi.org/10.1016/j.cbi.2022.109950 . .