Effects of mood stabilizer lithium on noradrenergic turnover in the prefrontal cortex of chronically stressed rats
Аутори
Gavrilović, LjubicaPopović, Nataša M.
Stojiljković, Vesna
Pejić, Snežana
Todorović, Ana
Pavlović, Ivan
Pantelić, Marija
Pajović, Snežana B.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Data about the dynamics of noradrenaline (NA) transmission, storage and degradation may be very important for understanding the reduction of functional deficiency of NA and maintaining the stability of NA levels in animals with depressive-like behavior treated with lithium. This study aimed to investigate the effects of mood stabilizer lithium on concentrations of NA in the prefrontal cortex (PFC), as well as behavior rats exposed to chronic restraint stress (CRS). In addition, this study examined the effects of lithium on protein levels of noradrenaline transporter (NET), vesicular monoamine transporter 2 (VMAT2) and catechol-O-methyltransferase (COMT), as well as the enzyme activity of monoamine oxidase A (MOA) in the PFC of chronically stressed rats. METHODS: The investigated parameters were quantified by Western blot analysis, CAT Research ELISA kits, and an assay of enzyme activity. Also, the forced swim test (FST) was used to assess the behavior of animals. RESULTS: We found that... lithium treatment decreased high protein levels of NET and VMAT2, as well as the enzyme activity of MOA in chronically stressed rats to the levels found in unstressed animals. In addition, lithium treatment decreased the concentration of NA (24%) and immobility in animals exposed to CRS. CONCLUSION: Our data confirm that lithium-induced modulation of prefrontal noradrenergic turnover and stabilized the behavior of chronically stressed rats.
Кључне речи:
chronic restraint stress / lithium / model / monoamine / noradrenaline / prefrontal cortex / ratsИзвор:
Neuroendocrinology Letters, 2021, 42, 3, 171-176Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200017 (Универзитет у Београду, Институт за нуклеарне науке Винча, Београд-Винча) (RS-MESTD-inst-2020-200017)
Институција/група
VinčaTY - JOUR AU - Gavrilović, Ljubica AU - Popović, Nataša M. AU - Stojiljković, Vesna AU - Pejić, Snežana AU - Todorović, Ana AU - Pavlović, Ivan AU - Pantelić, Marija AU - Pajović, Snežana B. PY - 2021 UR - https://vinar.vin.bg.ac.rs/handle/123456789/9959 AB - Data about the dynamics of noradrenaline (NA) transmission, storage and degradation may be very important for understanding the reduction of functional deficiency of NA and maintaining the stability of NA levels in animals with depressive-like behavior treated with lithium. This study aimed to investigate the effects of mood stabilizer lithium on concentrations of NA in the prefrontal cortex (PFC), as well as behavior rats exposed to chronic restraint stress (CRS). In addition, this study examined the effects of lithium on protein levels of noradrenaline transporter (NET), vesicular monoamine transporter 2 (VMAT2) and catechol-O-methyltransferase (COMT), as well as the enzyme activity of monoamine oxidase A (MOA) in the PFC of chronically stressed rats. METHODS: The investigated parameters were quantified by Western blot analysis, CAT Research ELISA kits, and an assay of enzyme activity. Also, the forced swim test (FST) was used to assess the behavior of animals. RESULTS: We found that lithium treatment decreased high protein levels of NET and VMAT2, as well as the enzyme activity of MOA in chronically stressed rats to the levels found in unstressed animals. In addition, lithium treatment decreased the concentration of NA (24%) and immobility in animals exposed to CRS. CONCLUSION: Our data confirm that lithium-induced modulation of prefrontal noradrenergic turnover and stabilized the behavior of chronically stressed rats. T2 - Neuroendocrinology Letters T1 - Effects of mood stabilizer lithium on noradrenergic turnover in the prefrontal cortex of chronically stressed rats VL - 42 IS - 3 SP - 171 EP - 176 UR - https://hdl.handle.net/21.15107/rcub_vinar_9959 ER -
@article{ author = "Gavrilović, Ljubica and Popović, Nataša M. and Stojiljković, Vesna and Pejić, Snežana and Todorović, Ana and Pavlović, Ivan and Pantelić, Marija and Pajović, Snežana B.", year = "2021", abstract = "Data about the dynamics of noradrenaline (NA) transmission, storage and degradation may be very important for understanding the reduction of functional deficiency of NA and maintaining the stability of NA levels in animals with depressive-like behavior treated with lithium. This study aimed to investigate the effects of mood stabilizer lithium on concentrations of NA in the prefrontal cortex (PFC), as well as behavior rats exposed to chronic restraint stress (CRS). In addition, this study examined the effects of lithium on protein levels of noradrenaline transporter (NET), vesicular monoamine transporter 2 (VMAT2) and catechol-O-methyltransferase (COMT), as well as the enzyme activity of monoamine oxidase A (MOA) in the PFC of chronically stressed rats. METHODS: The investigated parameters were quantified by Western blot analysis, CAT Research ELISA kits, and an assay of enzyme activity. Also, the forced swim test (FST) was used to assess the behavior of animals. RESULTS: We found that lithium treatment decreased high protein levels of NET and VMAT2, as well as the enzyme activity of MOA in chronically stressed rats to the levels found in unstressed animals. In addition, lithium treatment decreased the concentration of NA (24%) and immobility in animals exposed to CRS. CONCLUSION: Our data confirm that lithium-induced modulation of prefrontal noradrenergic turnover and stabilized the behavior of chronically stressed rats.", journal = "Neuroendocrinology Letters", title = "Effects of mood stabilizer lithium on noradrenergic turnover in the prefrontal cortex of chronically stressed rats", volume = "42", number = "3", pages = "171-176", url = "https://hdl.handle.net/21.15107/rcub_vinar_9959" }
Gavrilović, L., Popović, N. M., Stojiljković, V., Pejić, S., Todorović, A., Pavlović, I., Pantelić, M.,& Pajović, S. B.. (2021). Effects of mood stabilizer lithium on noradrenergic turnover in the prefrontal cortex of chronically stressed rats. in Neuroendocrinology Letters, 42(3), 171-176. https://hdl.handle.net/21.15107/rcub_vinar_9959
Gavrilović L, Popović NM, Stojiljković V, Pejić S, Todorović A, Pavlović I, Pantelić M, Pajović SB. Effects of mood stabilizer lithium on noradrenergic turnover in the prefrontal cortex of chronically stressed rats. in Neuroendocrinology Letters. 2021;42(3):171-176. https://hdl.handle.net/21.15107/rcub_vinar_9959 .
Gavrilović, Ljubica, Popović, Nataša M., Stojiljković, Vesna, Pejić, Snežana, Todorović, Ana, Pavlović, Ivan, Pantelić, Marija, Pajović, Snežana B., "Effects of mood stabilizer lithium on noradrenergic turnover in the prefrontal cortex of chronically stressed rats" in Neuroendocrinology Letters, 42, no. 3 (2021):171-176, https://hdl.handle.net/21.15107/rcub_vinar_9959 .