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dc.creatorKesavan, R.
dc.creatorChandel, S.
dc.creatorUpadhyay, S.
dc.creatorBendre, R.
dc.creatorGanugula, R.
dc.creatorPotunuru, U. R.
dc.creatorGiri, H.
dc.creatorSahu, G.
dc.creatorKumar, P. Uday
dc.creatorReddy, G. Bhanuprakash
dc.creatorJoksić, Gordana
dc.creatorBera, A. K.
dc.creatorDixit, Madhulika
dc.date.accessioned2018-03-01T16:46:45Z
dc.date.available2018-03-01T16:46:45Z
dc.date.issued2016
dc.identifier.issn0939-4753 (print)
dc.identifier.issn1590-3729 (electronic)
dc.identifier.urihttp://vinar.vin.bg.ac.rs/handle/123456789/993
dc.description.abstractBackground and aims: Studies suggest that Gentiana lutea (GL), and its component isovitexin, may exhibit anti-atherosclerotic properties. In this study we sought to investigate the protective mechanism of GL aqueous root extract and isovitexin on endothelial inflammation, smooth muscle cell migation, and on the onset and progression of atherosclerosis in streptozotocin (STZ)-induced diabetic rats. Methods and results: Our results show that both GL extract and isovitexin, block leukocyte adhesion and generation of reactive oxygen species in human umbilical vein endothelial cells (HUVECs) and rat aortic smooth muscle cells (RASMCs), following TNF-alpha and platelet derived growth factor-BB (PDGF-BB) challenges respectively. Both the extract and isovitexin blocked TNF-alpha induced expression of ICAM-1 and VCAM-1 in HUVECs. PDGF-BB induced migration of RASMCs and phospholipase C-gamma activation, were also abrogated by GL extract and isovitexin. Fura-2 based ratiometric measurements demonstrated that, both the extact, and isovitexin, inhibit PDGF-BB mediated intracellular calcium rise in RASMCs. Supplementation of regular diet with 2% GL root powder for STZ rats, reduced total cholesterol in blood. Oil Red O staining demonstrated decreased lipid accumulation in aortic wall of diabetic animals upon treatment with GL. Medial thickness and deposition of collagen in the aortic segment of diabetic rats were also reduced upon supplementation. Immunohistochemistry demonstrated reduced expression of vascular cell adhesion molecule-1 (VCAM-1), inducible nitric oxide synthase (iNOS), and vascular endothelial cadherin (VE-cadherin) in aortic segments of diabetic rats following GL treatment. Conclusions: Thus, our results support that GL root extract/powder and isovitexin exhibit anti-atherosclerotic activities. (C) 2016 The Italian Society of Diabetology, the Italian Society for the Study of Atherosclerosis, the Italian Society of Human Nutrition, and the Department of Clinical Medicine and Surgery, Federico II University. Published by Elsevier B.V. All rights reserved.en
dc.relationDepartment of Biotechnology, Government of India [BT/IN/EU/17/SN/2009]], Council for Scientific and Industrial Research (CSIR), Government of India [CSIR-SRA (13-8553A) 2012/POOL]
dc.rightsrestrictedAccessen
dc.sourceNutrition Metabolism and Cardiovascular Diseasesen
dc.subjectAtherosclerosisen
dc.subjectGentiana luteaen
dc.subjectIsovitexinen
dc.subjectEndothelial inflammationen
dc.subjectSmooth muscle cellen
dc.subjectPLC-gammaen
dc.subjectROSen
dc.titleGentiana lutea exerts anti-atherosclerotic effects by preventing endothelial inflammation and smooth muscle cell migrationen
dc.typearticleen
dcterms.abstractГири, Х.; Јоксић Гордана; Ганугула, Р.; Диxит, Мадхулика; Реддy, Г. Бханупракасх; Саху, Г.; Упадхyаy, С.; Цхандел, С.; Бендре, Р.; Кумар, П. Удаy; Кесаван, Р.; Бера, A. К.; Потунуру, У. Р.;
dc.citation.volume26
dc.citation.issue4
dc.citation.spage293
dc.citation.epage301
dc.identifier.wos000373525600003
dc.identifier.doi10.1016/j.numecd.2015.12.016
dc.citation.rankM21
dc.identifier.pmid26868432
dc.identifier.scopus2-s2.0-84956957810


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