Chicken genome modelling for the benefit of science
Samo za registrovane korisnike
2016
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In the early 21st century, genetic modification of chicken primordial germ cells (PGCs) had not been possible before their transfer and recovery through germ line. Chicken PGCs resist deliberate genetic modification, probably by silencing the transgenes in the genome. The use of mobile genetic elements (transposons) in genetic modification and germ-line transmission of PGCs has recently overcome this problem, so that PGCs can be used for further chicken genome modelling in order to study developmental biology, non-coding RNA (ncRNA) functions, viral DNA-RNA hybridisation, silencing of transgene expression, together with epigenetic modification, and gene function. Application of the transposons, viral integrase, zinc-finger nuclease and site-specific recombinase in whole genome elucidation is not sufficient. The chicken PGCs can be used as a model of choice to establish a new generation of methodology for genome modelling. For this purpose the livesome vectors are proposed.
Ključne reči:
chicken / genome modification / livesomeIzvor:
Worlds Poultry Science Journal, 2016, 72, 1, 81-91
DOI: 10.1017/S0043933915002652
ISSN: 0043-9339; 1743-4777
WoS: 000372263200006
Scopus: 2-s2.0-84955616153
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Institucija/grupa
VinčaTY - JOUR AU - Stupar, Milanko R. AU - Vidović, Vitomir AU - Lukač, Dragomir AU - Puvača, Nikola PY - 2016 UR - https://vinar.vin.bg.ac.rs/handle/123456789/972 AB - In the early 21st century, genetic modification of chicken primordial germ cells (PGCs) had not been possible before their transfer and recovery through germ line. Chicken PGCs resist deliberate genetic modification, probably by silencing the transgenes in the genome. The use of mobile genetic elements (transposons) in genetic modification and germ-line transmission of PGCs has recently overcome this problem, so that PGCs can be used for further chicken genome modelling in order to study developmental biology, non-coding RNA (ncRNA) functions, viral DNA-RNA hybridisation, silencing of transgene expression, together with epigenetic modification, and gene function. Application of the transposons, viral integrase, zinc-finger nuclease and site-specific recombinase in whole genome elucidation is not sufficient. The chicken PGCs can be used as a model of choice to establish a new generation of methodology for genome modelling. For this purpose the livesome vectors are proposed. T2 - Worlds Poultry Science Journal T1 - Chicken genome modelling for the benefit of science VL - 72 IS - 1 SP - 81 EP - 91 DO - 10.1017/S0043933915002652 ER -
@article{ author = "Stupar, Milanko R. and Vidović, Vitomir and Lukač, Dragomir and Puvača, Nikola", year = "2016", abstract = "In the early 21st century, genetic modification of chicken primordial germ cells (PGCs) had not been possible before their transfer and recovery through germ line. Chicken PGCs resist deliberate genetic modification, probably by silencing the transgenes in the genome. The use of mobile genetic elements (transposons) in genetic modification and germ-line transmission of PGCs has recently overcome this problem, so that PGCs can be used for further chicken genome modelling in order to study developmental biology, non-coding RNA (ncRNA) functions, viral DNA-RNA hybridisation, silencing of transgene expression, together with epigenetic modification, and gene function. Application of the transposons, viral integrase, zinc-finger nuclease and site-specific recombinase in whole genome elucidation is not sufficient. The chicken PGCs can be used as a model of choice to establish a new generation of methodology for genome modelling. For this purpose the livesome vectors are proposed.", journal = "Worlds Poultry Science Journal", title = "Chicken genome modelling for the benefit of science", volume = "72", number = "1", pages = "81-91", doi = "10.1017/S0043933915002652" }
Stupar, M. R., Vidović, V., Lukač, D.,& Puvača, N.. (2016). Chicken genome modelling for the benefit of science. in Worlds Poultry Science Journal, 72(1), 81-91. https://doi.org/10.1017/S0043933915002652
Stupar MR, Vidović V, Lukač D, Puvača N. Chicken genome modelling for the benefit of science. in Worlds Poultry Science Journal. 2016;72(1):81-91. doi:10.1017/S0043933915002652 .
Stupar, Milanko R., Vidović, Vitomir, Lukač, Dragomir, Puvača, Nikola, "Chicken genome modelling for the benefit of science" in Worlds Poultry Science Journal, 72, no. 1 (2016):81-91, https://doi.org/10.1017/S0043933915002652 . .