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dc.creatorDojčilović, Radovan
dc.creatorPajović, Jelena D.
dc.creatorBožanić, Dušan K.
dc.creatorVodnik, Vesna
dc.creatorDimitrijević-Branković, Suzana I.
dc.creatorMilosavljević, Aleksandar R.
dc.creatorKascakova, S.
dc.creatorRefregiers, M.
dc.creatorĐoković, Vladimir
dc.date.accessioned2018-03-01T16:44:48Z
dc.date.available2018-03-01T16:44:48Z
dc.date.issued2016
dc.identifier.issn0003-2654 (print)
dc.identifier.issn1364-5528 (electronic)
dc.identifier.urihttp://vinar.vin.bg.ac.rs/handle/123456789/971
dc.description.abstractThe investigation of the interaction of silver nanoparticles and live bacteria cells is of particular importance for understanding and controlling their bactericidal properties. In this study, the process of internalization of silver nanoparticles in Escherichia coli cells was followed by means of synchrotron excitation deep ultraviolet (DUV) fluorescence imaging. Antimicrobial nanostructures that can absorb and emit light in the UV region were prepared by functionalization of silver nanoparticles with tryptophan amino acid and used as environmentally sensitive fluorescent probes. The nanostructures were characterized by morphological (TEM) and spectroscopic methods (UV-vis, FTIR, XPS, and photoluminescence). The TEM images and the analyses of the UV-vis spectra suggested that the addition of tryptophan led to the formation of hybrid nanostructures with pronounced eccentricity and larger sizes with respect to that of the initial silver nanoparticles. The DUV imaging showed that it was possible to distinguish the fluorescent signal pertaining to silver-tryptophan nanostructures from the autofluorescence of the bacteria. The spatial resolution of the fluorescence images was 154 nm which was sufficient to perform analyses of the accumulation of the nanostructures within a single bacterium. The DUV imaging results imply that the tryptophan-functionalized silver nanoparticles interact with cell membranes via insertion of the amino acid into the phospholipid bilayer and enter the cells.en
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/45020/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172056/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/171020/RS//
dc.relationDISCO beamline of Synchrotron SOLEIL (France) [20120810]
dc.rightsopenAccessen
dc.sourceAnalysten
dc.titleA fluorescent nanoprobe for single bacterium tracking: functionalization of silver nanoparticles with tryptophan to probe the nanoparticle accumulation with single cell resolutionen
dc.typearticleen
dcterms.abstractКасцакова, С.; Дојчиловић Радован; Милосављевиц, A. Р.; Бозанић Душан; Пајовиц, Ј. Д.; Водник Весна; Димитријевиц-Бранковиц, С.; Рефрегиерс, М.; Ђоковић Владимир;
dc.citation.volume141
dc.citation.issue6
dc.citation.spage1988
dc.citation.epage1996
dc.identifier.wos000372259100015
dc.identifier.doi10.1039/c5an02358k
dc.citation.rankM21a
dc.identifier.pmid26858997
dc.identifier.scopus2-s2.0-84960371943
dc.identifier.fulltexthttp://vinar.rcub.bg.ac.rs/bitstream/handle/123456789/971/967.pdf


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