A fluorescent nanoprobe for single bacterium tracking: functionalization of silver nanoparticles with tryptophan to probe the nanoparticle accumulation with single cell resolution
Аутори
Dojčilović, RadovanPajović, Jelena D.
Božanić, Dušan K.
Vodnik, Vesna
Dimitrijević-Branković, Suzana I.
Milosavljević, Aleksandar R.
Kascakova, S.
Refregiers, M.
Đoković, Vladimir
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The investigation of the interaction of silver nanoparticles and live bacteria cells is of particular importance for understanding and controlling their bactericidal properties. In this study, the process of internalization of silver nanoparticles in Escherichia coli cells was followed by means of synchrotron excitation deep ultraviolet (DUV) fluorescence imaging. Antimicrobial nanostructures that can absorb and emit light in the UV region were prepared by functionalization of silver nanoparticles with tryptophan amino acid and used as environmentally sensitive fluorescent probes. The nanostructures were characterized by morphological (TEM) and spectroscopic methods (UV-vis, FTIR, XPS, and photoluminescence). The TEM images and the analyses of the UV-vis spectra suggested that the addition of tryptophan led to the formation of hybrid nanostructures with pronounced eccentricity and larger sizes with respect to that of the initial silver nanoparticles. The DUV imaging showed that it was ...possible to distinguish the fluorescent signal pertaining to silver-tryptophan nanostructures from the autofluorescence of the bacteria. The spatial resolution of the fluorescence images was 154 nm which was sufficient to perform analyses of the accumulation of the nanostructures within a single bacterium. The DUV imaging results imply that the tryptophan-functionalized silver nanoparticles interact with cell membranes via insertion of the amino acid into the phospholipid bilayer and enter the cells.
Извор:
Analyst, 2016, 141, 6, 1988-1996Финансирање / пројекти:
- Материјали редуковане димензионалности за ефикасну апсорпцију светлости и конверзију енергије (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-45020)
- Утицај величине, облика и структуре наночестица на њихова својства и својства нанокомпозита (RS-MESTD-Basic Research (BR or ON)-172056)
- Физика судара и фотопроцеса у атомским, (био)молекулским и нанодимензионим системима (RS-MESTD-Basic Research (BR or ON)-171020)
- DISCO beamline of Synchrotron SOLEIL (France) [20120810]
DOI: 10.1039/c5an02358k
ISSN: 0003-2654; 1364-5528
PubMed: 26858997
WoS: 000372259100015
Scopus: 2-s2.0-84960371943
Колекције
Институција/група
VinčaTY - JOUR AU - Dojčilović, Radovan AU - Pajović, Jelena D. AU - Božanić, Dušan K. AU - Vodnik, Vesna AU - Dimitrijević-Branković, Suzana I. AU - Milosavljević, Aleksandar R. AU - Kascakova, S. AU - Refregiers, M. AU - Đoković, Vladimir PY - 2016 UR - https://vinar.vin.bg.ac.rs/handle/123456789/971 AB - The investigation of the interaction of silver nanoparticles and live bacteria cells is of particular importance for understanding and controlling their bactericidal properties. In this study, the process of internalization of silver nanoparticles in Escherichia coli cells was followed by means of synchrotron excitation deep ultraviolet (DUV) fluorescence imaging. Antimicrobial nanostructures that can absorb and emit light in the UV region were prepared by functionalization of silver nanoparticles with tryptophan amino acid and used as environmentally sensitive fluorescent probes. The nanostructures were characterized by morphological (TEM) and spectroscopic methods (UV-vis, FTIR, XPS, and photoluminescence). The TEM images and the analyses of the UV-vis spectra suggested that the addition of tryptophan led to the formation of hybrid nanostructures with pronounced eccentricity and larger sizes with respect to that of the initial silver nanoparticles. The DUV imaging showed that it was possible to distinguish the fluorescent signal pertaining to silver-tryptophan nanostructures from the autofluorescence of the bacteria. The spatial resolution of the fluorescence images was 154 nm which was sufficient to perform analyses of the accumulation of the nanostructures within a single bacterium. The DUV imaging results imply that the tryptophan-functionalized silver nanoparticles interact with cell membranes via insertion of the amino acid into the phospholipid bilayer and enter the cells. T2 - Analyst T1 - A fluorescent nanoprobe for single bacterium tracking: functionalization of silver nanoparticles with tryptophan to probe the nanoparticle accumulation with single cell resolution VL - 141 IS - 6 SP - 1988 EP - 1996 DO - 10.1039/c5an02358k ER -
@article{ author = "Dojčilović, Radovan and Pajović, Jelena D. and Božanić, Dušan K. and Vodnik, Vesna and Dimitrijević-Branković, Suzana I. and Milosavljević, Aleksandar R. and Kascakova, S. and Refregiers, M. and Đoković, Vladimir", year = "2016", abstract = "The investigation of the interaction of silver nanoparticles and live bacteria cells is of particular importance for understanding and controlling their bactericidal properties. In this study, the process of internalization of silver nanoparticles in Escherichia coli cells was followed by means of synchrotron excitation deep ultraviolet (DUV) fluorescence imaging. Antimicrobial nanostructures that can absorb and emit light in the UV region were prepared by functionalization of silver nanoparticles with tryptophan amino acid and used as environmentally sensitive fluorescent probes. The nanostructures were characterized by morphological (TEM) and spectroscopic methods (UV-vis, FTIR, XPS, and photoluminescence). The TEM images and the analyses of the UV-vis spectra suggested that the addition of tryptophan led to the formation of hybrid nanostructures with pronounced eccentricity and larger sizes with respect to that of the initial silver nanoparticles. The DUV imaging showed that it was possible to distinguish the fluorescent signal pertaining to silver-tryptophan nanostructures from the autofluorescence of the bacteria. The spatial resolution of the fluorescence images was 154 nm which was sufficient to perform analyses of the accumulation of the nanostructures within a single bacterium. The DUV imaging results imply that the tryptophan-functionalized silver nanoparticles interact with cell membranes via insertion of the amino acid into the phospholipid bilayer and enter the cells.", journal = "Analyst", title = "A fluorescent nanoprobe for single bacterium tracking: functionalization of silver nanoparticles with tryptophan to probe the nanoparticle accumulation with single cell resolution", volume = "141", number = "6", pages = "1988-1996", doi = "10.1039/c5an02358k" }
Dojčilović, R., Pajović, J. D., Božanić, D. K., Vodnik, V., Dimitrijević-Branković, S. I., Milosavljević, A. R., Kascakova, S., Refregiers, M.,& Đoković, V.. (2016). A fluorescent nanoprobe for single bacterium tracking: functionalization of silver nanoparticles with tryptophan to probe the nanoparticle accumulation with single cell resolution. in Analyst, 141(6), 1988-1996. https://doi.org/10.1039/c5an02358k
Dojčilović R, Pajović JD, Božanić DK, Vodnik V, Dimitrijević-Branković SI, Milosavljević AR, Kascakova S, Refregiers M, Đoković V. A fluorescent nanoprobe for single bacterium tracking: functionalization of silver nanoparticles with tryptophan to probe the nanoparticle accumulation with single cell resolution. in Analyst. 2016;141(6):1988-1996. doi:10.1039/c5an02358k .
Dojčilović, Radovan, Pajović, Jelena D., Božanić, Dušan K., Vodnik, Vesna, Dimitrijević-Branković, Suzana I., Milosavljević, Aleksandar R., Kascakova, S., Refregiers, M., Đoković, Vladimir, "A fluorescent nanoprobe for single bacterium tracking: functionalization of silver nanoparticles with tryptophan to probe the nanoparticle accumulation with single cell resolution" in Analyst, 141, no. 6 (2016):1988-1996, https://doi.org/10.1039/c5an02358k . .