Yellow gentian root extract provokes concentration- and time-dependent response in peripheral blood mononuclear cells
Аутори
Valenta-Šobot, AnaDrakulić, Dunja R.
Joksić, Gordana
Miletić Vukajlović, Jadranka
Savić, Jasmina
Potočnik, Jelena
Filipović Tričković, Jelena G.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Yellow gentian (Gentiana lutea L.), a medicinal plant widely used in traditional medicine, displays multiple biological effects, ranging from beneficial to toxic. Since many promising applications have been reported so far, our aim was to evaluate its potential concentration- and time- dependent cytotoxic and genotoxic effects in vitro . To that end we exposed human peripheral blood mononuclear cells to 0.5, 1, and 2 mg/mL of yellow gentian root extract (YGRE) to determine its effects on oxidative stress parameters [pro/antioxidant balance (PAB) and lipid peroxidation], DNA damage (alkaline comet assay and chromosome aberrations), and cell viability (trypan blue exclusion test). Cell viability decreased with increasing concentrations and treatment duration. Only the lowest YGRE concentration (0.5 mg/mL) increased oxidative stress but produced minor DNA damage and cytotoxicity. At higher concentrations, redox parameters returned to near control values. The percentage of chromosome aberr...ations and percentage of DNA in the comet tail increased with increased YGRE concentration after 48 h and declined after 72 h of treatment. This points to the activation of DNA repair mechanism (homologous recombination), evidenced by the formation of chromosomal radial figures after 72 h of treatment with the highest YGRE concentration of 2 mg/mL. Our results suggest that YGRE, despite induction of cytotoxic and genotoxic effects, activates cell repair mechanisms that counter oxidative and DNA lesions and induce cell death in highly damaged cells. Therefore, observed protective effects of yellow gentian after longer exposure could be a result of activated repair and removal of cells with irreparable damage.
Кључне речи:
cytotoxicity / genotoxicity / Gentiana lutea L. / homologous recombination / redox parametersИзвор:
Archives of Industrial Hygiene and Toxicology, 2020, 71, 4, 320-328Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200017 (Универзитет у Београду, Институт за нуклеарне науке Винча, Београд-Винча) (RS-MESTD-inst-2020-200017)
DOI: 10.2478/aiht-2020-71-3476
ISSN: 0004-1254
PubMed: 33410776
WoS: 000605843200005
Scopus: 2-s2.0-85099382217
Колекције
Институција/група
VinčaTY - JOUR AU - Valenta-Šobot, Ana AU - Drakulić, Dunja R. AU - Joksić, Gordana AU - Miletić Vukajlović, Jadranka AU - Savić, Jasmina AU - Potočnik, Jelena AU - Filipović Tričković, Jelena G. PY - 2020 UR - https://vinar.vin.bg.ac.rs/handle/123456789/9523 AB - Yellow gentian (Gentiana lutea L.), a medicinal plant widely used in traditional medicine, displays multiple biological effects, ranging from beneficial to toxic. Since many promising applications have been reported so far, our aim was to evaluate its potential concentration- and time- dependent cytotoxic and genotoxic effects in vitro . To that end we exposed human peripheral blood mononuclear cells to 0.5, 1, and 2 mg/mL of yellow gentian root extract (YGRE) to determine its effects on oxidative stress parameters [pro/antioxidant balance (PAB) and lipid peroxidation], DNA damage (alkaline comet assay and chromosome aberrations), and cell viability (trypan blue exclusion test). Cell viability decreased with increasing concentrations and treatment duration. Only the lowest YGRE concentration (0.5 mg/mL) increased oxidative stress but produced minor DNA damage and cytotoxicity. At higher concentrations, redox parameters returned to near control values. The percentage of chromosome aberrations and percentage of DNA in the comet tail increased with increased YGRE concentration after 48 h and declined after 72 h of treatment. This points to the activation of DNA repair mechanism (homologous recombination), evidenced by the formation of chromosomal radial figures after 72 h of treatment with the highest YGRE concentration of 2 mg/mL. Our results suggest that YGRE, despite induction of cytotoxic and genotoxic effects, activates cell repair mechanisms that counter oxidative and DNA lesions and induce cell death in highly damaged cells. Therefore, observed protective effects of yellow gentian after longer exposure could be a result of activated repair and removal of cells with irreparable damage. T2 - Archives of Industrial Hygiene and Toxicology T1 - Yellow gentian root extract provokes concentration- and time-dependent response in peripheral blood mononuclear cells VL - 71 IS - 4 SP - 320 EP - 328 DO - 10.2478/aiht-2020-71-3476 ER -
@article{ author = "Valenta-Šobot, Ana and Drakulić, Dunja R. and Joksić, Gordana and Miletić Vukajlović, Jadranka and Savić, Jasmina and Potočnik, Jelena and Filipović Tričković, Jelena G.", year = "2020", abstract = "Yellow gentian (Gentiana lutea L.), a medicinal plant widely used in traditional medicine, displays multiple biological effects, ranging from beneficial to toxic. Since many promising applications have been reported so far, our aim was to evaluate its potential concentration- and time- dependent cytotoxic and genotoxic effects in vitro . To that end we exposed human peripheral blood mononuclear cells to 0.5, 1, and 2 mg/mL of yellow gentian root extract (YGRE) to determine its effects on oxidative stress parameters [pro/antioxidant balance (PAB) and lipid peroxidation], DNA damage (alkaline comet assay and chromosome aberrations), and cell viability (trypan blue exclusion test). Cell viability decreased with increasing concentrations and treatment duration. Only the lowest YGRE concentration (0.5 mg/mL) increased oxidative stress but produced minor DNA damage and cytotoxicity. At higher concentrations, redox parameters returned to near control values. The percentage of chromosome aberrations and percentage of DNA in the comet tail increased with increased YGRE concentration after 48 h and declined after 72 h of treatment. This points to the activation of DNA repair mechanism (homologous recombination), evidenced by the formation of chromosomal radial figures after 72 h of treatment with the highest YGRE concentration of 2 mg/mL. Our results suggest that YGRE, despite induction of cytotoxic and genotoxic effects, activates cell repair mechanisms that counter oxidative and DNA lesions and induce cell death in highly damaged cells. Therefore, observed protective effects of yellow gentian after longer exposure could be a result of activated repair and removal of cells with irreparable damage.", journal = "Archives of Industrial Hygiene and Toxicology", title = "Yellow gentian root extract provokes concentration- and time-dependent response in peripheral blood mononuclear cells", volume = "71", number = "4", pages = "320-328", doi = "10.2478/aiht-2020-71-3476" }
Valenta-Šobot, A., Drakulić, D. R., Joksić, G., Miletić Vukajlović, J., Savić, J., Potočnik, J.,& Filipović Tričković, J. G.. (2020). Yellow gentian root extract provokes concentration- and time-dependent response in peripheral blood mononuclear cells. in Archives of Industrial Hygiene and Toxicology, 71(4), 320-328. https://doi.org/10.2478/aiht-2020-71-3476
Valenta-Šobot A, Drakulić DR, Joksić G, Miletić Vukajlović J, Savić J, Potočnik J, Filipović Tričković JG. Yellow gentian root extract provokes concentration- and time-dependent response in peripheral blood mononuclear cells. in Archives of Industrial Hygiene and Toxicology. 2020;71(4):320-328. doi:10.2478/aiht-2020-71-3476 .
Valenta-Šobot, Ana, Drakulić, Dunja R., Joksić, Gordana, Miletić Vukajlović, Jadranka, Savić, Jasmina, Potočnik, Jelena, Filipović Tričković, Jelena G., "Yellow gentian root extract provokes concentration- and time-dependent response in peripheral blood mononuclear cells" in Archives of Industrial Hygiene and Toxicology, 71, no. 4 (2020):320-328, https://doi.org/10.2478/aiht-2020-71-3476 . .