Effects of ghrelin on protein expression of antioxidative enzymes and iNOS in the rat liver
2014
Аутори
Dobutović, BranislavaSudar, Emina
Tepavčević, Snežana
Đorđević, Jelena D.
Đorđević, Ana D.
Radoičić, Marija B.
Isenović, Esma R.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Introduction: We investigated the effects of ghrelin on protein expression of the liver antioxidant enzymes superoxide dismutases (SODs), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR), nuclear factor kappa B (NF kappa B) and inducible nitric oxide synthase (iNOS). Furthermore, we aimed to investigate whether extracellular regulated protein kinase (ERK1/2) and protein kinase B (Akt) are involved in ghrelin-regulated liver antioxidant enzymes and iNOS protein expression. Material and methods: Male Wistar rats were treated with ghrelin (0.3 nmol/5 mu l) injected into the lateral cerebral ventricle every 24 h for 5 days, and 2 h after the last treatment the animals were sacrificed and the liver excised. The Western blot method was used to determine expression of antioxidant enzymes, iNOS, phosphorylation of Akt, ERK1/2 and nuclear factor kappa B (NF kappa B) subunits 50 and 65. Results: There was significantly higher protein expression of CuZnSOD (p LT 0.001)..., MnSOD (p LT 0.001), CAT (p LT 0.001), GPx, (p LT 0.001), and GR (p LT 0.01) in the liver isolated from ghrelin-treated animals compared with control animals. In contrast, ghrelin significantly (p LT 0.01) reduced protein expression of iNOS. In addition, phosphorylation of NF kappa B subunits p65 and p50 was significantly (p LT 0.001 for p65; p LT 0.05 for p50) reduced by ghrelin when compared with controls. Phosphorylation of ERK1/2 and of Akt was significantly higher in ghrelin-treated than in control animals (p LT 0.05 for ERK1/2; p LT 0.01 for Akt). Conclusions: The results show that activation of Akt and ERK1/2 is involved in ghrelin-mediated regulation of protein expression of antioxidant enzymes and iNOS in the rat liver.
Кључне речи:
Akt / ERK1/2 / superoxide dismutase / catalase / oxidative stress / nuclear factor kappa BИзвор:
Archives of Medical Science, 2014, 10, 4, 806-816Финансирање / пројекти:
- Хормонска регулација експресије и активности азот оксид синтазе и натријум-калијумове пумпе у експерименталним моделима инсулинске резистенције, дијабетеса и кардиоваскуларних поремећаја (RS-MESTD-Basic Research (BR or ON)-173033)
- Модулација сигналних путева који контролишу интрацелуларни енергетски баланс у терапији тумора и неуро-имуно-ендокриних поремећаја (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41025)
- Дефинисање кластера молекулских биомаркера за побољшану дијагностику и терапију поремећаја расположења (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41029)
DOI: 10.5114/aoms.2014.44872
ISSN: 1734-1922; 1896-9151
PubMed: 25276168
WoS: 000341274400024
Scopus: 2-s2.0-84907454877
Колекције
Институција/група
VinčaTY - JOUR AU - Dobutović, Branislava AU - Sudar, Emina AU - Tepavčević, Snežana AU - Đorđević, Jelena D. AU - Đorđević, Ana D. AU - Radoičić, Marija B. AU - Isenović, Esma R. PY - 2014 UR - https://vinar.vin.bg.ac.rs/handle/123456789/93 AB - Introduction: We investigated the effects of ghrelin on protein expression of the liver antioxidant enzymes superoxide dismutases (SODs), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR), nuclear factor kappa B (NF kappa B) and inducible nitric oxide synthase (iNOS). Furthermore, we aimed to investigate whether extracellular regulated protein kinase (ERK1/2) and protein kinase B (Akt) are involved in ghrelin-regulated liver antioxidant enzymes and iNOS protein expression. Material and methods: Male Wistar rats were treated with ghrelin (0.3 nmol/5 mu l) injected into the lateral cerebral ventricle every 24 h for 5 days, and 2 h after the last treatment the animals were sacrificed and the liver excised. The Western blot method was used to determine expression of antioxidant enzymes, iNOS, phosphorylation of Akt, ERK1/2 and nuclear factor kappa B (NF kappa B) subunits 50 and 65. Results: There was significantly higher protein expression of CuZnSOD (p LT 0.001), MnSOD (p LT 0.001), CAT (p LT 0.001), GPx, (p LT 0.001), and GR (p LT 0.01) in the liver isolated from ghrelin-treated animals compared with control animals. In contrast, ghrelin significantly (p LT 0.01) reduced protein expression of iNOS. In addition, phosphorylation of NF kappa B subunits p65 and p50 was significantly (p LT 0.001 for p65; p LT 0.05 for p50) reduced by ghrelin when compared with controls. Phosphorylation of ERK1/2 and of Akt was significantly higher in ghrelin-treated than in control animals (p LT 0.05 for ERK1/2; p LT 0.01 for Akt). Conclusions: The results show that activation of Akt and ERK1/2 is involved in ghrelin-mediated regulation of protein expression of antioxidant enzymes and iNOS in the rat liver. T2 - Archives of Medical Science T1 - Effects of ghrelin on protein expression of antioxidative enzymes and iNOS in the rat liver VL - 10 IS - 4 SP - 806 EP - 816 DO - 10.5114/aoms.2014.44872 ER -
@article{ author = "Dobutović, Branislava and Sudar, Emina and Tepavčević, Snežana and Đorđević, Jelena D. and Đorđević, Ana D. and Radoičić, Marija B. and Isenović, Esma R.", year = "2014", abstract = "Introduction: We investigated the effects of ghrelin on protein expression of the liver antioxidant enzymes superoxide dismutases (SODs), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR), nuclear factor kappa B (NF kappa B) and inducible nitric oxide synthase (iNOS). Furthermore, we aimed to investigate whether extracellular regulated protein kinase (ERK1/2) and protein kinase B (Akt) are involved in ghrelin-regulated liver antioxidant enzymes and iNOS protein expression. Material and methods: Male Wistar rats were treated with ghrelin (0.3 nmol/5 mu l) injected into the lateral cerebral ventricle every 24 h for 5 days, and 2 h after the last treatment the animals were sacrificed and the liver excised. The Western blot method was used to determine expression of antioxidant enzymes, iNOS, phosphorylation of Akt, ERK1/2 and nuclear factor kappa B (NF kappa B) subunits 50 and 65. Results: There was significantly higher protein expression of CuZnSOD (p LT 0.001), MnSOD (p LT 0.001), CAT (p LT 0.001), GPx, (p LT 0.001), and GR (p LT 0.01) in the liver isolated from ghrelin-treated animals compared with control animals. In contrast, ghrelin significantly (p LT 0.01) reduced protein expression of iNOS. In addition, phosphorylation of NF kappa B subunits p65 and p50 was significantly (p LT 0.001 for p65; p LT 0.05 for p50) reduced by ghrelin when compared with controls. Phosphorylation of ERK1/2 and of Akt was significantly higher in ghrelin-treated than in control animals (p LT 0.05 for ERK1/2; p LT 0.01 for Akt). Conclusions: The results show that activation of Akt and ERK1/2 is involved in ghrelin-mediated regulation of protein expression of antioxidant enzymes and iNOS in the rat liver.", journal = "Archives of Medical Science", title = "Effects of ghrelin on protein expression of antioxidative enzymes and iNOS in the rat liver", volume = "10", number = "4", pages = "806-816", doi = "10.5114/aoms.2014.44872" }
Dobutović, B., Sudar, E., Tepavčević, S., Đorđević, J. D., Đorđević, A. D., Radoičić, M. B.,& Isenović, E. R.. (2014). Effects of ghrelin on protein expression of antioxidative enzymes and iNOS in the rat liver. in Archives of Medical Science, 10(4), 806-816. https://doi.org/10.5114/aoms.2014.44872
Dobutović B, Sudar E, Tepavčević S, Đorđević JD, Đorđević AD, Radoičić MB, Isenović ER. Effects of ghrelin on protein expression of antioxidative enzymes and iNOS in the rat liver. in Archives of Medical Science. 2014;10(4):806-816. doi:10.5114/aoms.2014.44872 .
Dobutović, Branislava, Sudar, Emina, Tepavčević, Snežana, Đorđević, Jelena D., Đorđević, Ana D., Radoičić, Marija B., Isenović, Esma R., "Effects of ghrelin on protein expression of antioxidative enzymes and iNOS in the rat liver" in Archives of Medical Science, 10, no. 4 (2014):806-816, https://doi.org/10.5114/aoms.2014.44872 . .