Tryptophan-functionalized gold nanoparticles for deep UV imaging of microbial cells
Samo za registrovane korisnike
2015
Autori
Pajović, Jelena D.Dojčilović, Radovan
Božanić, Dušan K.
Kaščakova, Slavka
Refregiers, Matthieu
Dimitrijević-Branković, Suzana I.
Vodnik, Vesna
Milosavljević, Aleksandar R.
Piscopiello, Emanuela
Luyt, Adriaan S.
Đoković, Vladimir
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Biocompatible fluorescent nanostructures were prepared by a functionalization of gold nanoparticles with the amino acid tryptophan. The gold-tryptophan bioconjugates were investigated by TEM and HRTEM and various spectroscopy methods (XPS, FTIR, UV-vis and photoluminescence). It was found that the gold nanoparticles, initially 8 nm in diameter, aggregate in the presence of the amino acid. From the XPS and FTIR spectroscopy results, it was concluded that the tryptophan gold interactions mainly take place via indole and carboxyl groups. Although the indole group is involved in the interaction with the gold surfaces, the tryptophan-gold hybrids showed strong fluorescence due to the presence of multilayers of tryptophan. Deep ultra violet (DUV) imaging performed at the SOLEIL synchrotron showed that It is possible to detect these hybrid nanostructures within Escherichia coli cells. (c) 2015 Elsevier B.V. All rights reserved.
Ključne reči:
Gold nanoparticles / Tryptophan / Functionalization / Fluorescence imaging / E. coliIzvor:
Colloids and Surfaces. B: Biointerfaces, 2015, 135, 742-750Izdavač:
- Elsevier
Finansiranje / projekti:
- Materijali redukovane dimenzionalnosti za efikasnu apsorpciju svetlosti i konverziju energije (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-45020)
- Uticaj veličine, oblika i strukture nanočestica na njihova svojstva i svojstva nanokompozita (RS-MESTD-Basic Research (BR or ON)-172056)
- Fizika sudara i fotoprocesa u atomskim, (bio)molekulskim i nanodimenzionim sistemima (RS-MESTD-Basic Research (BR or ON)-171020)
- [20120810]
DOI: 10.1016/j.colsurfb.2015.08.050
ISSN: 0927-7765; 1873-4367
PubMed: 26340364
WoS: 000367410200091
Scopus: 2-s2.0-84940494285
Kolekcije
Institucija/grupa
VinčaTY - JOUR AU - Pajović, Jelena D. AU - Dojčilović, Radovan AU - Božanić, Dušan K. AU - Kaščakova, Slavka AU - Refregiers, Matthieu AU - Dimitrijević-Branković, Suzana I. AU - Vodnik, Vesna AU - Milosavljević, Aleksandar R. AU - Piscopiello, Emanuela AU - Luyt, Adriaan S. AU - Đoković, Vladimir PY - 2015 UR - https://vinar.vin.bg.ac.rs/handle/123456789/875 AB - Biocompatible fluorescent nanostructures were prepared by a functionalization of gold nanoparticles with the amino acid tryptophan. The gold-tryptophan bioconjugates were investigated by TEM and HRTEM and various spectroscopy methods (XPS, FTIR, UV-vis and photoluminescence). It was found that the gold nanoparticles, initially 8 nm in diameter, aggregate in the presence of the amino acid. From the XPS and FTIR spectroscopy results, it was concluded that the tryptophan gold interactions mainly take place via indole and carboxyl groups. Although the indole group is involved in the interaction with the gold surfaces, the tryptophan-gold hybrids showed strong fluorescence due to the presence of multilayers of tryptophan. Deep ultra violet (DUV) imaging performed at the SOLEIL synchrotron showed that It is possible to detect these hybrid nanostructures within Escherichia coli cells. (c) 2015 Elsevier B.V. All rights reserved. PB - Elsevier T2 - Colloids and Surfaces. B: Biointerfaces T1 - Tryptophan-functionalized gold nanoparticles for deep UV imaging of microbial cells VL - 135 SP - 742 EP - 750 DO - 10.1016/j.colsurfb.2015.08.050 ER -
@article{ author = "Pajović, Jelena D. and Dojčilović, Radovan and Božanić, Dušan K. and Kaščakova, Slavka and Refregiers, Matthieu and Dimitrijević-Branković, Suzana I. and Vodnik, Vesna and Milosavljević, Aleksandar R. and Piscopiello, Emanuela and Luyt, Adriaan S. and Đoković, Vladimir", year = "2015", abstract = "Biocompatible fluorescent nanostructures were prepared by a functionalization of gold nanoparticles with the amino acid tryptophan. The gold-tryptophan bioconjugates were investigated by TEM and HRTEM and various spectroscopy methods (XPS, FTIR, UV-vis and photoluminescence). It was found that the gold nanoparticles, initially 8 nm in diameter, aggregate in the presence of the amino acid. From the XPS and FTIR spectroscopy results, it was concluded that the tryptophan gold interactions mainly take place via indole and carboxyl groups. Although the indole group is involved in the interaction with the gold surfaces, the tryptophan-gold hybrids showed strong fluorescence due to the presence of multilayers of tryptophan. Deep ultra violet (DUV) imaging performed at the SOLEIL synchrotron showed that It is possible to detect these hybrid nanostructures within Escherichia coli cells. (c) 2015 Elsevier B.V. All rights reserved.", publisher = "Elsevier", journal = "Colloids and Surfaces. B: Biointerfaces", title = "Tryptophan-functionalized gold nanoparticles for deep UV imaging of microbial cells", volume = "135", pages = "742-750", doi = "10.1016/j.colsurfb.2015.08.050" }
Pajović, J. D., Dojčilović, R., Božanić, D. K., Kaščakova, S., Refregiers, M., Dimitrijević-Branković, S. I., Vodnik, V., Milosavljević, A. R., Piscopiello, E., Luyt, A. S.,& Đoković, V.. (2015). Tryptophan-functionalized gold nanoparticles for deep UV imaging of microbial cells. in Colloids and Surfaces. B: Biointerfaces Elsevier., 135, 742-750. https://doi.org/10.1016/j.colsurfb.2015.08.050
Pajović JD, Dojčilović R, Božanić DK, Kaščakova S, Refregiers M, Dimitrijević-Branković SI, Vodnik V, Milosavljević AR, Piscopiello E, Luyt AS, Đoković V. Tryptophan-functionalized gold nanoparticles for deep UV imaging of microbial cells. in Colloids and Surfaces. B: Biointerfaces. 2015;135:742-750. doi:10.1016/j.colsurfb.2015.08.050 .
Pajović, Jelena D., Dojčilović, Radovan, Božanić, Dušan K., Kaščakova, Slavka, Refregiers, Matthieu, Dimitrijević-Branković, Suzana I., Vodnik, Vesna, Milosavljević, Aleksandar R., Piscopiello, Emanuela, Luyt, Adriaan S., Đoković, Vladimir, "Tryptophan-functionalized gold nanoparticles for deep UV imaging of microbial cells" in Colloids and Surfaces. B: Biointerfaces, 135 (2015):742-750, https://doi.org/10.1016/j.colsurfb.2015.08.050 . .