Tryptophan-functionalized gold nanoparticles for deep UV imaging of microbial cells
AuthorsPajović, Jelena D.
Božanić, Dušan K.
Dimitrijević-Branković, Suzana I.
Milosavljević, Aleksandar R.
Luyt, Adriaan S.
MetadataShow full item record
Biocompatible fluorescent nanostructures were prepared by a functionalization of gold nanoparticles with the amino acid tryptophan. The gold-tryptophan bioconjugates were investigated by TEM and HRTEM and various spectroscopy methods (XPS, FTIR, UV-vis and photoluminescence). It was found that the gold nanoparticles, initially 8 nm in diameter, aggregate in the presence of the amino acid. From the XPS and FTIR spectroscopy results, it was concluded that the tryptophan gold interactions mainly take place via indole and carboxyl groups. Although the indole group is involved in the interaction with the gold surfaces, the tryptophan-gold hybrids showed strong fluorescence due to the presence of multilayers of tryptophan. Deep ultra violet (DUV) imaging performed at the SOLEIL synchrotron showed that It is possible to detect these hybrid nanostructures within Escherichia coli cells. (c) 2015 Elsevier B.V. All rights reserved.
Keywords:Gold nanoparticles / Tryptophan / Functionalization / Fluorescence imaging / E. coli
Source:Colloids and Surfaces. B: Biointerfaces, 2015, 135, 742-750
- Materials of Reduced Dimensions for Efficient Light Harvesting and Energy conversion (RS-45020)
- Size-, shape- and structure- dependent properties of nanoparticles and nanocomposites (RS-172056)
- Physics of collisions and photo processes in atomic, (bio)molecular and nanosized systems (RS-171020)