Micro Rna-21 Expression Levels in Invasive Breast Carcinoma with a Non-Invasive Component
Аутори
Petrović, NinaJovanović-Ćupić, Snežana P.
Brajušković, Goran
Lukić, Silvana
Roganović, Jelena
Krajnović, Milena M.
Mandušić, Vesna
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Invasive ductal carcinomas with a non-invasive component (IDC-DCIS) are classified as a group of invasive breast carcinomas, together with pure invasive ductal carcinomas of the breast (IDC). MicroRNA-21 (miR-21) has been characterized as a factor of breast cancer invasiveness, however the difference in miR-21 expression levels between IDC-DCIS and pure IDC tumors and the correlations with standard diagnostic and prognostic parameters inside the IDC-DCIS group are unknown. Our aim was to determine if miR-21 had the ability to distinguish these two invasive breast cancer groups. Levels of miR-21 expression were measured by a stem-loop quantitative Real-Time PCR (RT-qPCR) method. Expression levels of estrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor 2 (Her-2) and proliferative index Ki-67 were evaluated by immunohistochemistry. IDC-DCIS tumors had significantly lower levels of miR-21 expression in grade 2 (P=0.003, Mann-Whitney U test), ER positiv...e (P=0.025, Mann-Whitney U test) and PR positive tumors (P=0.024, Mann-Whitney U test) than pure IDCs. miR-21 levels showed a different pattern of expression in IDC-DCIS compared to IDC tumors, which is based on the difference in miR-21 expression between Her-2 negative and Her-2 positive IDC-DCIS tumors (P=0.030, Mann-Whitney U test) and high negative correlation of miR-21 levels with PR levels (rho=-0.886, P=0.006, Spearman correlation). According to our results, IDC-DCIS breast carcinomas act in a different manner in pure IDC tumors with regard to the relations between miR-21 expression levels and the standard diagnostic and prognostic parameters, such as Her-2 status, ER and PR status and protein levels.
Кључне речи:
Invasive ductal carcinoma with non-invasive component (IDC-DCIS) / miR-21 expression levels / the difference between IDC-DCIS and IDCИзвор:
Archives of Biological Sciences, 2015, 67, 4, 1285-1295Финансирање / пројекти:
- Молекуларне детерминанте за дизајн тумор маркера (RS-MESTD-Basic Research (BR or ON)-173049)
DOI: 10.2298/ABS150327105P
ISSN: 0354-4664; 1821-4339
WoS: 000366795700020
Scopus: 2-s2.0-84951790728
Колекције
Институција/група
VinčaTY - JOUR AU - Petrović, Nina AU - Jovanović-Ćupić, Snežana P. AU - Brajušković, Goran AU - Lukić, Silvana AU - Roganović, Jelena AU - Krajnović, Milena M. AU - Mandušić, Vesna PY - 2015 UR - https://vinar.vin.bg.ac.rs/handle/123456789/862 AB - Invasive ductal carcinomas with a non-invasive component (IDC-DCIS) are classified as a group of invasive breast carcinomas, together with pure invasive ductal carcinomas of the breast (IDC). MicroRNA-21 (miR-21) has been characterized as a factor of breast cancer invasiveness, however the difference in miR-21 expression levels between IDC-DCIS and pure IDC tumors and the correlations with standard diagnostic and prognostic parameters inside the IDC-DCIS group are unknown. Our aim was to determine if miR-21 had the ability to distinguish these two invasive breast cancer groups. Levels of miR-21 expression were measured by a stem-loop quantitative Real-Time PCR (RT-qPCR) method. Expression levels of estrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor 2 (Her-2) and proliferative index Ki-67 were evaluated by immunohistochemistry. IDC-DCIS tumors had significantly lower levels of miR-21 expression in grade 2 (P=0.003, Mann-Whitney U test), ER positive (P=0.025, Mann-Whitney U test) and PR positive tumors (P=0.024, Mann-Whitney U test) than pure IDCs. miR-21 levels showed a different pattern of expression in IDC-DCIS compared to IDC tumors, which is based on the difference in miR-21 expression between Her-2 negative and Her-2 positive IDC-DCIS tumors (P=0.030, Mann-Whitney U test) and high negative correlation of miR-21 levels with PR levels (rho=-0.886, P=0.006, Spearman correlation). According to our results, IDC-DCIS breast carcinomas act in a different manner in pure IDC tumors with regard to the relations between miR-21 expression levels and the standard diagnostic and prognostic parameters, such as Her-2 status, ER and PR status and protein levels. T2 - Archives of Biological Sciences T1 - Micro Rna-21 Expression Levels in Invasive Breast Carcinoma with a Non-Invasive Component VL - 67 IS - 4 SP - 1285 EP - 1295 DO - 10.2298/ABS150327105P ER -
@article{ author = "Petrović, Nina and Jovanović-Ćupić, Snežana P. and Brajušković, Goran and Lukić, Silvana and Roganović, Jelena and Krajnović, Milena M. and Mandušić, Vesna", year = "2015", abstract = "Invasive ductal carcinomas with a non-invasive component (IDC-DCIS) are classified as a group of invasive breast carcinomas, together with pure invasive ductal carcinomas of the breast (IDC). MicroRNA-21 (miR-21) has been characterized as a factor of breast cancer invasiveness, however the difference in miR-21 expression levels between IDC-DCIS and pure IDC tumors and the correlations with standard diagnostic and prognostic parameters inside the IDC-DCIS group are unknown. Our aim was to determine if miR-21 had the ability to distinguish these two invasive breast cancer groups. Levels of miR-21 expression were measured by a stem-loop quantitative Real-Time PCR (RT-qPCR) method. Expression levels of estrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor 2 (Her-2) and proliferative index Ki-67 were evaluated by immunohistochemistry. IDC-DCIS tumors had significantly lower levels of miR-21 expression in grade 2 (P=0.003, Mann-Whitney U test), ER positive (P=0.025, Mann-Whitney U test) and PR positive tumors (P=0.024, Mann-Whitney U test) than pure IDCs. miR-21 levels showed a different pattern of expression in IDC-DCIS compared to IDC tumors, which is based on the difference in miR-21 expression between Her-2 negative and Her-2 positive IDC-DCIS tumors (P=0.030, Mann-Whitney U test) and high negative correlation of miR-21 levels with PR levels (rho=-0.886, P=0.006, Spearman correlation). According to our results, IDC-DCIS breast carcinomas act in a different manner in pure IDC tumors with regard to the relations between miR-21 expression levels and the standard diagnostic and prognostic parameters, such as Her-2 status, ER and PR status and protein levels.", journal = "Archives of Biological Sciences", title = "Micro Rna-21 Expression Levels in Invasive Breast Carcinoma with a Non-Invasive Component", volume = "67", number = "4", pages = "1285-1295", doi = "10.2298/ABS150327105P" }
Petrović, N., Jovanović-Ćupić, S. P., Brajušković, G., Lukić, S., Roganović, J., Krajnović, M. M.,& Mandušić, V.. (2015). Micro Rna-21 Expression Levels in Invasive Breast Carcinoma with a Non-Invasive Component. in Archives of Biological Sciences, 67(4), 1285-1295. https://doi.org/10.2298/ABS150327105P
Petrović N, Jovanović-Ćupić SP, Brajušković G, Lukić S, Roganović J, Krajnović MM, Mandušić V. Micro Rna-21 Expression Levels in Invasive Breast Carcinoma with a Non-Invasive Component. in Archives of Biological Sciences. 2015;67(4):1285-1295. doi:10.2298/ABS150327105P .
Petrović, Nina, Jovanović-Ćupić, Snežana P., Brajušković, Goran, Lukić, Silvana, Roganović, Jelena, Krajnović, Milena M., Mandušić, Vesna, "Micro Rna-21 Expression Levels in Invasive Breast Carcinoma with a Non-Invasive Component" in Archives of Biological Sciences, 67, no. 4 (2015):1285-1295, https://doi.org/10.2298/ABS150327105P . .