Biological outcomes of γ-radiation induced DNA damages in breast and lung cancer cells pretreated with free radical scavengers
Нема приказа
Аутори
Petković, VladanaKeta, Otilija D.
Vidosavljević, Marija Z.
Incerti, Sebastien
Ristić-Fira, Aleksandra
Petrović, Ivan M.
Чланак у часопису (Објављена верзија)
,
© 2019 Taylor & Francis Group, LLC
Метаподаци
Приказ свих података о документуАпстракт
Purpose: Investigation of effects on DNA of γ-irradiated human cancer cells pretreated with free radical scavengers is aimed to create reference data which would enable assessment of the relative efficiency of high linear energy transfer (LET) radiations used in hadron therapy, i.e. protons and carbon ions. Materials and methods: MCF-7 breast and HTB177 lung cancer cells are irradiated with γ-rays. To minimize indirect effects of irradiation, dimethyl sulfoxide (DMSO) or glycerol are applied as free radical scavengers. Biological response to irradiation is evaluated through clonogenic cell survival, immunocytochemical and cell cycle analysis, as well as expression of proteins involved in DNA damage response. Results: Examined cell lines reveal similar level of radioresistance. Application of scavengers leads to the rise of cell survival and decreases the number of DNA double strand breaks in irradiated cells. Differences in cell cycle and protein expression between the two cell lines a...re probably caused by different DNA damage repair mechanisms that are activated. Conclusion: The obtained results show that DMSO and glycerol have good scavenging capacity, and may be used to minimize DNA damage induced by free radicals. Therefore, they will be used as the reference for comparison with high LET irradiations, as well as good experimental data suitable for validation of numerical simulations. © 2019, © 2019 Taylor & Francis Group, LLC.
Кључне речи:
Breast cancer cells / non-small cell lung cancer cells / gamma-rays / free radical scavengersИзвор:
International Journal of Radiation Biology, 2019, 95, 3, 274-285Финансирање / пројекти:
- Радиосензитивност хуманог генома (RS-173046)
- Физика високих енергија са детектором CMS (RS-171019)
DOI: 10.1080/09553002.2019.1549753
ISSN: 0955-3002
PubMed: 30451568
WoS: 000463581500003
Scopus: 2-s2.0-85060603904
Колекције
Институција/група
VinčaTY - JOUR AU - Petković, Vladana AU - Keta, Otilija D. AU - Vidosavljević, Marija Z. AU - Incerti, Sebastien AU - Ristić-Fira, Aleksandra AU - Petrović, Ivan M. PY - 2019 UR - https://vinar.vin.bg.ac.rs/handle/123456789/8414 AB - Purpose: Investigation of effects on DNA of γ-irradiated human cancer cells pretreated with free radical scavengers is aimed to create reference data which would enable assessment of the relative efficiency of high linear energy transfer (LET) radiations used in hadron therapy, i.e. protons and carbon ions. Materials and methods: MCF-7 breast and HTB177 lung cancer cells are irradiated with γ-rays. To minimize indirect effects of irradiation, dimethyl sulfoxide (DMSO) or glycerol are applied as free radical scavengers. Biological response to irradiation is evaluated through clonogenic cell survival, immunocytochemical and cell cycle analysis, as well as expression of proteins involved in DNA damage response. Results: Examined cell lines reveal similar level of radioresistance. Application of scavengers leads to the rise of cell survival and decreases the number of DNA double strand breaks in irradiated cells. Differences in cell cycle and protein expression between the two cell lines are probably caused by different DNA damage repair mechanisms that are activated. Conclusion: The obtained results show that DMSO and glycerol have good scavenging capacity, and may be used to minimize DNA damage induced by free radicals. Therefore, they will be used as the reference for comparison with high LET irradiations, as well as good experimental data suitable for validation of numerical simulations. © 2019, © 2019 Taylor & Francis Group, LLC. T2 - International Journal of Radiation Biology T1 - Biological outcomes of γ-radiation induced DNA damages in breast and lung cancer cells pretreated with free radical scavengers VL - 95 IS - 3 SP - 274 EP - 285 DO - 10.1080/09553002.2019.1549753 ER -
@article{ author = "Petković, Vladana and Keta, Otilija D. and Vidosavljević, Marija Z. and Incerti, Sebastien and Ristić-Fira, Aleksandra and Petrović, Ivan M.", year = "2019", abstract = "Purpose: Investigation of effects on DNA of γ-irradiated human cancer cells pretreated with free radical scavengers is aimed to create reference data which would enable assessment of the relative efficiency of high linear energy transfer (LET) radiations used in hadron therapy, i.e. protons and carbon ions. Materials and methods: MCF-7 breast and HTB177 lung cancer cells are irradiated with γ-rays. To minimize indirect effects of irradiation, dimethyl sulfoxide (DMSO) or glycerol are applied as free radical scavengers. Biological response to irradiation is evaluated through clonogenic cell survival, immunocytochemical and cell cycle analysis, as well as expression of proteins involved in DNA damage response. Results: Examined cell lines reveal similar level of radioresistance. Application of scavengers leads to the rise of cell survival and decreases the number of DNA double strand breaks in irradiated cells. Differences in cell cycle and protein expression between the two cell lines are probably caused by different DNA damage repair mechanisms that are activated. Conclusion: The obtained results show that DMSO and glycerol have good scavenging capacity, and may be used to minimize DNA damage induced by free radicals. Therefore, they will be used as the reference for comparison with high LET irradiations, as well as good experimental data suitable for validation of numerical simulations. © 2019, © 2019 Taylor & Francis Group, LLC.", journal = "International Journal of Radiation Biology", title = "Biological outcomes of γ-radiation induced DNA damages in breast and lung cancer cells pretreated with free radical scavengers", volume = "95", number = "3", pages = "274-285", doi = "10.1080/09553002.2019.1549753" }
Petković, V., Keta, O. D., Vidosavljević, M. Z., Incerti, S., Ristić-Fira, A.,& Petrović, I. M.. (2019). Biological outcomes of γ-radiation induced DNA damages in breast and lung cancer cells pretreated with free radical scavengers. in International Journal of Radiation Biology, 95(3), 274-285. https://doi.org/10.1080/09553002.2019.1549753
Petković V, Keta OD, Vidosavljević MZ, Incerti S, Ristić-Fira A, Petrović IM. Biological outcomes of γ-radiation induced DNA damages in breast and lung cancer cells pretreated with free radical scavengers. in International Journal of Radiation Biology. 2019;95(3):274-285. doi:10.1080/09553002.2019.1549753 .
Petković, Vladana, Keta, Otilija D., Vidosavljević, Marija Z., Incerti, Sebastien, Ristić-Fira, Aleksandra, Petrović, Ivan M., "Biological outcomes of γ-radiation induced DNA damages in breast and lung cancer cells pretreated with free radical scavengers" in International Journal of Radiation Biology, 95, no. 3 (2019):274-285, https://doi.org/10.1080/09553002.2019.1549753 . .