Recasting as a booster of Ag-Pd alloy cytotoxicity: Induction of cell senescence prior to mass cell death
Authors
Čairović, AleksandraStanimirović, Dragan
Krajnović, Tamara T.
Dojčinović, Biljana P.
Maksimović, Vesna
Cvijović-Alagić, Ivana
Article (Published version)
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The biological quality and chemical composition of alloys used in dental practice change during heat treatment. Often the residues of the previous cast are not disposed of but are reused and recycled until consumed. Thus, manufactured dental restorations have modified biological quality and chemical composition, and compromised biocompatibility. The aim of this study was to investigate the influence of repeated casting on the cytotoxicity of the silver-palladium (Ag-Pd) alloy. Our results showed that repeated casting of the Ag-Pd dental alloy affected its biocompatibility by promoting toxicity against transformed fibroblasts in a contact-independent manner. A strong decrease in cell proliferation, induction of senescence and massive cell death were observed in cultures exposed only to a medium previously incubated with dental alloy samples. The obtained data indicated that toxicity mediated by the accumulation of the Ag, Pd, Cu and Zn cations released from the Ag-Pd material was enhanc...ed by recasting. The induction of cell senescence and subsequent apoptotic and necrotic death were accompanied by amplified intracellular production of reactive oxygen and nitrogen species, suggesting their involvement in the cell destruction process. Therefore, compromised biocompatibility after recasting with the Ag-Pd alloy can be the cause of serious local cell destruction, as observed in clinical practice. © 2019 by the Serbian Biological Society.
Keywords:
dental alloys / cytotoxicity / necrosis / recasting / reactive oxygen speciesSource:
Archives of Biological Sciences, 2019, 71, 2, 347-356Funding / projects:
- Synthesis, processing and characterization of nanostructured materials for application in the field of energy, mechanical engineering, environmental protection and biomedicine (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-45012)
- Molecular mechanisms of physiological and pharmacological control of inflammation and cancer (RS-MESTD-Basic Research (BR or ON)-173013)
DOI: 10.2298/ABS190305017C
ISSN: 0354-4664; 1821-4339
WoS: 000471069700017
Scopus: 2-s2.0-85067112829
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VinčaTY - JOUR AU - Čairović, Aleksandra AU - Stanimirović, Dragan AU - Krajnović, Tamara T. AU - Dojčinović, Biljana P. AU - Maksimović, Vesna AU - Cvijović-Alagić, Ivana PY - 2019 UR - https://vinar.vin.bg.ac.rs/handle/123456789/8341 AB - The biological quality and chemical composition of alloys used in dental practice change during heat treatment. Often the residues of the previous cast are not disposed of but are reused and recycled until consumed. Thus, manufactured dental restorations have modified biological quality and chemical composition, and compromised biocompatibility. The aim of this study was to investigate the influence of repeated casting on the cytotoxicity of the silver-palladium (Ag-Pd) alloy. Our results showed that repeated casting of the Ag-Pd dental alloy affected its biocompatibility by promoting toxicity against transformed fibroblasts in a contact-independent manner. A strong decrease in cell proliferation, induction of senescence and massive cell death were observed in cultures exposed only to a medium previously incubated with dental alloy samples. The obtained data indicated that toxicity mediated by the accumulation of the Ag, Pd, Cu and Zn cations released from the Ag-Pd material was enhanced by recasting. The induction of cell senescence and subsequent apoptotic and necrotic death were accompanied by amplified intracellular production of reactive oxygen and nitrogen species, suggesting their involvement in the cell destruction process. Therefore, compromised biocompatibility after recasting with the Ag-Pd alloy can be the cause of serious local cell destruction, as observed in clinical practice. © 2019 by the Serbian Biological Society. T2 - Archives of Biological Sciences T1 - Recasting as a booster of Ag-Pd alloy cytotoxicity: Induction of cell senescence prior to mass cell death VL - 71 IS - 2 SP - 347 EP - 356 DO - 10.2298/ABS190305017C ER -
@article{ author = "Čairović, Aleksandra and Stanimirović, Dragan and Krajnović, Tamara T. and Dojčinović, Biljana P. and Maksimović, Vesna and Cvijović-Alagić, Ivana", year = "2019", abstract = "The biological quality and chemical composition of alloys used in dental practice change during heat treatment. Often the residues of the previous cast are not disposed of but are reused and recycled until consumed. Thus, manufactured dental restorations have modified biological quality and chemical composition, and compromised biocompatibility. The aim of this study was to investigate the influence of repeated casting on the cytotoxicity of the silver-palladium (Ag-Pd) alloy. Our results showed that repeated casting of the Ag-Pd dental alloy affected its biocompatibility by promoting toxicity against transformed fibroblasts in a contact-independent manner. A strong decrease in cell proliferation, induction of senescence and massive cell death were observed in cultures exposed only to a medium previously incubated with dental alloy samples. The obtained data indicated that toxicity mediated by the accumulation of the Ag, Pd, Cu and Zn cations released from the Ag-Pd material was enhanced by recasting. The induction of cell senescence and subsequent apoptotic and necrotic death were accompanied by amplified intracellular production of reactive oxygen and nitrogen species, suggesting their involvement in the cell destruction process. Therefore, compromised biocompatibility after recasting with the Ag-Pd alloy can be the cause of serious local cell destruction, as observed in clinical practice. © 2019 by the Serbian Biological Society.", journal = "Archives of Biological Sciences", title = "Recasting as a booster of Ag-Pd alloy cytotoxicity: Induction of cell senescence prior to mass cell death", volume = "71", number = "2", pages = "347-356", doi = "10.2298/ABS190305017C" }
Čairović, A., Stanimirović, D., Krajnović, T. T., Dojčinović, B. P., Maksimović, V.,& Cvijović-Alagić, I.. (2019). Recasting as a booster of Ag-Pd alloy cytotoxicity: Induction of cell senescence prior to mass cell death. in Archives of Biological Sciences, 71(2), 347-356. https://doi.org/10.2298/ABS190305017C
Čairović A, Stanimirović D, Krajnović TT, Dojčinović BP, Maksimović V, Cvijović-Alagić I. Recasting as a booster of Ag-Pd alloy cytotoxicity: Induction of cell senescence prior to mass cell death. in Archives of Biological Sciences. 2019;71(2):347-356. doi:10.2298/ABS190305017C .
Čairović, Aleksandra, Stanimirović, Dragan, Krajnović, Tamara T., Dojčinović, Biljana P., Maksimović, Vesna, Cvijović-Alagić, Ivana, "Recasting as a booster of Ag-Pd alloy cytotoxicity: Induction of cell senescence prior to mass cell death" in Archives of Biological Sciences, 71, no. 2 (2019):347-356, https://doi.org/10.2298/ABS190305017C . .