Antibacterial photodynamic activity of carbon quantum dots/polydimethylsiloxane nanocomposites against Staphylococcus aureus, Escherichia coli and Klebsiella pneumoniae
Authorized Users Only
2019
Authors
Marković, Zoran M.Kovačova, Maria
Humpoliček, Petr
Budimir, Milica
Vajdak, Jan
Kubat, Pavel
Mičušik, Matej
Švajdlenkova, Helena
Danko, Martin
Capakova, Zdenka
Lehocky, Marian
Todorović-Marković, Biljana
Špitalsky, Zdenko
Article (Published version)
,
© 2019 Elsevier B.V.
Metadata
Show full item recordAbstract
Despite great efforts, the design of antibacterial surfaces is still a challenge. In this work, results of structural, mechanical, cytotoxic and antibacterial activities of hydrophobic carbon quantum dots/polydimethylsiloxane surfaces are presented. Antibacterial action of this surface is based on the generation of reactive oxygen species which cause bacteria damage by oxidative stress. At the same time, this surface was not cytotoxic towards the NIH/3T3 cells. Swelling-encapsulation-shrink method is applied for encapsulation of hydrophobic carbon quantum dots in medical grade silicone-polydimethylsiloxane. XPS and photoluminescence spectroscopy analyses confirm that hydrophobic carbon quantum dots have been encapsulated successfully into polydimethylsiloxane polymer matrix. Based on stress-strain test the improvement of mechanical properties of these nanocomposites is established. It is shown by electron paramagnetic resonance spectroscopy and luminescence method that nanocomposite ge...nerates singlet oxygen initiated by 470 nm blue light irradiation. Antibacterial testing shows the nanocomposite in the form of foil kills Staphylococcus aureus, Escherichia coli and Klebsiella pneumoniae and is very effective after only a 15 min irradiation. © 2019 Elsevier B.V.
Keywords:
Hydrophobic carbon quantum dots / Medical grade polydimethylsiloxane / Antibacterial surfaces / Visible light sterilization / Antimicrobial photodynamic therapySource:
Photodiagnosis and Photodynamic Therapy, 2019, 26, 342-349Funding / projects:
- SASPRO Programme [1237/02/02-b]
- People Programme (Marie Curie Actions) European Union's Seventh Framework Programme under REA [609427]
- VEGA [2/0093/16]
- Ministry of Education, Youth and Sports of the Czech Republic Program NPU I [LO1504]
- Czech Science Foundation [19-09721S]
- Thin films of single wall carbon nanotubes and graphene for electronic application (RS-MESTD-Basic Research (BR or ON)-172003)
- bilateral project Serbia-Slovakia [SK-SRB-2016-0038]
- multilateral scientific and technological cooperation in the Danube region [DS-2016-021]
Note:
- Post-print version available at: http://vinar.vin.bg.ac.rs/handle/123456789/8187
- Link to erratum: https://vinar.vin.bg.ac.rs/handle/123456789/9615
Related info:
- Referenced by
https://vinar.vin.bg.ac.rs/handle/123456789/9615 - Referenced by
https://vinar.vin.bg.ac.rs/handle/123456789/8187
DOI: 10.1016/j.pdpdt.2019.04.019
ISSN: 1572-1000
PubMed: 31022579
WoS: 000474330400058
Scopus: 2-s2.0-85065202835
Collections
Institution/Community
VinčaTY - JOUR AU - Marković, Zoran M. AU - Kovačova, Maria AU - Humpoliček, Petr AU - Budimir, Milica AU - Vajdak, Jan AU - Kubat, Pavel AU - Mičušik, Matej AU - Švajdlenkova, Helena AU - Danko, Martin AU - Capakova, Zdenka AU - Lehocky, Marian AU - Todorović-Marković, Biljana AU - Špitalsky, Zdenko PY - 2019 UR - https://vinar.vin.bg.ac.rs/handle/123456789/8174 AB - Despite great efforts, the design of antibacterial surfaces is still a challenge. In this work, results of structural, mechanical, cytotoxic and antibacterial activities of hydrophobic carbon quantum dots/polydimethylsiloxane surfaces are presented. Antibacterial action of this surface is based on the generation of reactive oxygen species which cause bacteria damage by oxidative stress. At the same time, this surface was not cytotoxic towards the NIH/3T3 cells. Swelling-encapsulation-shrink method is applied for encapsulation of hydrophobic carbon quantum dots in medical grade silicone-polydimethylsiloxane. XPS and photoluminescence spectroscopy analyses confirm that hydrophobic carbon quantum dots have been encapsulated successfully into polydimethylsiloxane polymer matrix. Based on stress-strain test the improvement of mechanical properties of these nanocomposites is established. It is shown by electron paramagnetic resonance spectroscopy and luminescence method that nanocomposite generates singlet oxygen initiated by 470 nm blue light irradiation. Antibacterial testing shows the nanocomposite in the form of foil kills Staphylococcus aureus, Escherichia coli and Klebsiella pneumoniae and is very effective after only a 15 min irradiation. © 2019 Elsevier B.V. T2 - Photodiagnosis and Photodynamic Therapy T1 - Antibacterial photodynamic activity of carbon quantum dots/polydimethylsiloxane nanocomposites against Staphylococcus aureus, Escherichia coli and Klebsiella pneumoniae VL - 26 SP - 342 EP - 349 DO - 10.1016/j.pdpdt.2019.04.019 ER -
@article{ author = "Marković, Zoran M. and Kovačova, Maria and Humpoliček, Petr and Budimir, Milica and Vajdak, Jan and Kubat, Pavel and Mičušik, Matej and Švajdlenkova, Helena and Danko, Martin and Capakova, Zdenka and Lehocky, Marian and Todorović-Marković, Biljana and Špitalsky, Zdenko", year = "2019", abstract = "Despite great efforts, the design of antibacterial surfaces is still a challenge. In this work, results of structural, mechanical, cytotoxic and antibacterial activities of hydrophobic carbon quantum dots/polydimethylsiloxane surfaces are presented. Antibacterial action of this surface is based on the generation of reactive oxygen species which cause bacteria damage by oxidative stress. At the same time, this surface was not cytotoxic towards the NIH/3T3 cells. Swelling-encapsulation-shrink method is applied for encapsulation of hydrophobic carbon quantum dots in medical grade silicone-polydimethylsiloxane. XPS and photoluminescence spectroscopy analyses confirm that hydrophobic carbon quantum dots have been encapsulated successfully into polydimethylsiloxane polymer matrix. Based on stress-strain test the improvement of mechanical properties of these nanocomposites is established. It is shown by electron paramagnetic resonance spectroscopy and luminescence method that nanocomposite generates singlet oxygen initiated by 470 nm blue light irradiation. Antibacterial testing shows the nanocomposite in the form of foil kills Staphylococcus aureus, Escherichia coli and Klebsiella pneumoniae and is very effective after only a 15 min irradiation. © 2019 Elsevier B.V.", journal = "Photodiagnosis and Photodynamic Therapy", title = "Antibacterial photodynamic activity of carbon quantum dots/polydimethylsiloxane nanocomposites against Staphylococcus aureus, Escherichia coli and Klebsiella pneumoniae", volume = "26", pages = "342-349", doi = "10.1016/j.pdpdt.2019.04.019" }
Marković, Z. M., Kovačova, M., Humpoliček, P., Budimir, M., Vajdak, J., Kubat, P., Mičušik, M., Švajdlenkova, H., Danko, M., Capakova, Z., Lehocky, M., Todorović-Marković, B.,& Špitalsky, Z.. (2019). Antibacterial photodynamic activity of carbon quantum dots/polydimethylsiloxane nanocomposites against Staphylococcus aureus, Escherichia coli and Klebsiella pneumoniae. in Photodiagnosis and Photodynamic Therapy, 26, 342-349. https://doi.org/10.1016/j.pdpdt.2019.04.019
Marković ZM, Kovačova M, Humpoliček P, Budimir M, Vajdak J, Kubat P, Mičušik M, Švajdlenkova H, Danko M, Capakova Z, Lehocky M, Todorović-Marković B, Špitalsky Z. Antibacterial photodynamic activity of carbon quantum dots/polydimethylsiloxane nanocomposites against Staphylococcus aureus, Escherichia coli and Klebsiella pneumoniae. in Photodiagnosis and Photodynamic Therapy. 2019;26:342-349. doi:10.1016/j.pdpdt.2019.04.019 .
Marković, Zoran M., Kovačova, Maria, Humpoliček, Petr, Budimir, Milica, Vajdak, Jan, Kubat, Pavel, Mičušik, Matej, Švajdlenkova, Helena, Danko, Martin, Capakova, Zdenka, Lehocky, Marian, Todorović-Marković, Biljana, Špitalsky, Zdenko, "Antibacterial photodynamic activity of carbon quantum dots/polydimethylsiloxane nanocomposites against Staphylococcus aureus, Escherichia coli and Klebsiella pneumoniae" in Photodiagnosis and Photodynamic Therapy, 26 (2019):342-349, https://doi.org/10.1016/j.pdpdt.2019.04.019 . .