99mTc–bisphosphonate–coated magnetic nanoparticles as potential theranostic nanoagent
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2019
Authors
Mirković, Marija D.Radović, Magdalena
Stanković, Dragana
Milanović, Zorana
Janković, Drina
Matović, Milovan D.
Jeremić, Marija
Antić, Bratislav
Vranješ-Đurić, Sanja
Article (Published version)
,
© 2019 Elsevier B.V.
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Novel theranostic nanoplatform is expected to integrate imaging for guiding and monitoring of the tumor therapy with great therapeutic efficacy and fewer side effects. Here we describe the preparation of a multifunctional 99mTc–bisphosphonate–coated magnetic nanoparticles (MNPs) based on Fe3O4 and coated with two hydrophilic bisphosphonate ligands, i.e., methylene diphosphonate (MDP) and 1–hydroxyethane-1,1- diphosphonate (HEDP). The presence of the bisphosphonates on the MNPs surface, enabled their biocompatibility, colloidal stability and successful binding of the radionuclide. The morphology, size, structure, surface charge and magnetic properties of obtained bisphosphonate–coated Fe3O4 MNPs were characterized by transmission electron microscopy, X–ray powder diffraction, dynamic light scattering, laser Doppler electrophoresis, Fourier transform infrared spectroscopy and vibrating sample magnetometer. The specific power absorption values for Fe3O4–MDP and Fe3O4–HEDP were 113 W/g and... 141 W/g, respectively, indicated their heating ability under applied magnetic field. Coated MNPs were radiolabeled with 99mTc using stannous chloride as the reducing agent in a reproducible high yield (95% for Fe3O4–MDP and 97% for Fe3O4–HEDP MNPs) and were remained stable in saline and human serum for 24 h. Ex vivo biodistribution studies presented significant liver and spleen uptake in healthy Wistar rats after intravenous administration at all examined time points due to the colloidal nature of both 99mTc–MNPs. Results of scintigraphy studies are in accordance with ex vivo biodistribution studies, demonstrating high in vivo stability of radiolabeled MNPs and therefore results of both methods were proved as accurate information on the biodistribution profile of investigated MNPs. Overall, in vitro and in vivo stability as well as heating ability, indicate that biocompatible radiolabeled bisphosphonate magnetic nanoparticles exhibit promising potential as a theranostic nanoagent. © 2019 Elsevier B.V.
Keywords:
Magnetic nanoparticles / Bisphosphonates / Radiolabeling / Theranostic agent / ScintigraphySource:
Materials Science and Engineering: C, 2019, 102, 124-133Funding / projects:
- Magnetic and radionuclide labeled nanostructured materials for medical applications (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-45015)
- COST Action RADIOMAG [TD1402]
- Strengthening of the MagBioVin Research and Innovation Team for Development of Novel Approaches for Tumour Therapy based on Nanostructured Materials (EU-FP7-621375)
DOI: 10.1016/j.msec.2019.04.034
ISSN: 0928-4931
PubMed: 31146983
WoS: 000472241700013
Scopus: 2-s2.0-85064531232
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VinčaTY - JOUR AU - Mirković, Marija D. AU - Radović, Magdalena AU - Stanković, Dragana AU - Milanović, Zorana AU - Janković, Drina AU - Matović, Milovan D. AU - Jeremić, Marija AU - Antić, Bratislav AU - Vranješ-Đurić, Sanja PY - 2019 UR - https://vinar.vin.bg.ac.rs/handle/123456789/8156 AB - Novel theranostic nanoplatform is expected to integrate imaging for guiding and monitoring of the tumor therapy with great therapeutic efficacy and fewer side effects. Here we describe the preparation of a multifunctional 99mTc–bisphosphonate–coated magnetic nanoparticles (MNPs) based on Fe3O4 and coated with two hydrophilic bisphosphonate ligands, i.e., methylene diphosphonate (MDP) and 1–hydroxyethane-1,1- diphosphonate (HEDP). The presence of the bisphosphonates on the MNPs surface, enabled their biocompatibility, colloidal stability and successful binding of the radionuclide. The morphology, size, structure, surface charge and magnetic properties of obtained bisphosphonate–coated Fe3O4 MNPs were characterized by transmission electron microscopy, X–ray powder diffraction, dynamic light scattering, laser Doppler electrophoresis, Fourier transform infrared spectroscopy and vibrating sample magnetometer. The specific power absorption values for Fe3O4–MDP and Fe3O4–HEDP were 113 W/g and 141 W/g, respectively, indicated their heating ability under applied magnetic field. Coated MNPs were radiolabeled with 99mTc using stannous chloride as the reducing agent in a reproducible high yield (95% for Fe3O4–MDP and 97% for Fe3O4–HEDP MNPs) and were remained stable in saline and human serum for 24 h. Ex vivo biodistribution studies presented significant liver and spleen uptake in healthy Wistar rats after intravenous administration at all examined time points due to the colloidal nature of both 99mTc–MNPs. Results of scintigraphy studies are in accordance with ex vivo biodistribution studies, demonstrating high in vivo stability of radiolabeled MNPs and therefore results of both methods were proved as accurate information on the biodistribution profile of investigated MNPs. Overall, in vitro and in vivo stability as well as heating ability, indicate that biocompatible radiolabeled bisphosphonate magnetic nanoparticles exhibit promising potential as a theranostic nanoagent. © 2019 Elsevier B.V. T2 - Materials Science and Engineering: C T1 - 99mTc–bisphosphonate–coated magnetic nanoparticles as potential theranostic nanoagent VL - 102 SP - 124 EP - 133 DO - 10.1016/j.msec.2019.04.034 ER -
@article{ author = "Mirković, Marija D. and Radović, Magdalena and Stanković, Dragana and Milanović, Zorana and Janković, Drina and Matović, Milovan D. and Jeremić, Marija and Antić, Bratislav and Vranješ-Đurić, Sanja", year = "2019", abstract = "Novel theranostic nanoplatform is expected to integrate imaging for guiding and monitoring of the tumor therapy with great therapeutic efficacy and fewer side effects. Here we describe the preparation of a multifunctional 99mTc–bisphosphonate–coated magnetic nanoparticles (MNPs) based on Fe3O4 and coated with two hydrophilic bisphosphonate ligands, i.e., methylene diphosphonate (MDP) and 1–hydroxyethane-1,1- diphosphonate (HEDP). The presence of the bisphosphonates on the MNPs surface, enabled their biocompatibility, colloidal stability and successful binding of the radionuclide. The morphology, size, structure, surface charge and magnetic properties of obtained bisphosphonate–coated Fe3O4 MNPs were characterized by transmission electron microscopy, X–ray powder diffraction, dynamic light scattering, laser Doppler electrophoresis, Fourier transform infrared spectroscopy and vibrating sample magnetometer. The specific power absorption values for Fe3O4–MDP and Fe3O4–HEDP were 113 W/g and 141 W/g, respectively, indicated their heating ability under applied magnetic field. Coated MNPs were radiolabeled with 99mTc using stannous chloride as the reducing agent in a reproducible high yield (95% for Fe3O4–MDP and 97% for Fe3O4–HEDP MNPs) and were remained stable in saline and human serum for 24 h. Ex vivo biodistribution studies presented significant liver and spleen uptake in healthy Wistar rats after intravenous administration at all examined time points due to the colloidal nature of both 99mTc–MNPs. Results of scintigraphy studies are in accordance with ex vivo biodistribution studies, demonstrating high in vivo stability of radiolabeled MNPs and therefore results of both methods were proved as accurate information on the biodistribution profile of investigated MNPs. Overall, in vitro and in vivo stability as well as heating ability, indicate that biocompatible radiolabeled bisphosphonate magnetic nanoparticles exhibit promising potential as a theranostic nanoagent. © 2019 Elsevier B.V.", journal = "Materials Science and Engineering: C", title = "99mTc–bisphosphonate–coated magnetic nanoparticles as potential theranostic nanoagent", volume = "102", pages = "124-133", doi = "10.1016/j.msec.2019.04.034" }
Mirković, M. D., Radović, M., Stanković, D., Milanović, Z., Janković, D., Matović, M. D., Jeremić, M., Antić, B.,& Vranješ-Đurić, S.. (2019). 99mTc–bisphosphonate–coated magnetic nanoparticles as potential theranostic nanoagent. in Materials Science and Engineering: C, 102, 124-133. https://doi.org/10.1016/j.msec.2019.04.034
Mirković MD, Radović M, Stanković D, Milanović Z, Janković D, Matović MD, Jeremić M, Antić B, Vranješ-Đurić S. 99mTc–bisphosphonate–coated magnetic nanoparticles as potential theranostic nanoagent. in Materials Science and Engineering: C. 2019;102:124-133. doi:10.1016/j.msec.2019.04.034 .
Mirković, Marija D., Radović, Magdalena, Stanković, Dragana, Milanović, Zorana, Janković, Drina, Matović, Milovan D., Jeremić, Marija, Antić, Bratislav, Vranješ-Đurić, Sanja, "99mTc–bisphosphonate–coated magnetic nanoparticles as potential theranostic nanoagent" in Materials Science and Engineering: C, 102 (2019):124-133, https://doi.org/10.1016/j.msec.2019.04.034 . .