Subchronic methionine load induces oxidative stress and provokes biochemical and histological changes in the rat liver tissue
Samo za registrovane korisnike
2018
Autori
Stojanović, MarijaTodorović, Dajana D.
Šćepanović, Ljiljana
Mitrović, Dušan M.
Borozan, Sunčica Z.
Dragutinović, Vesna V.
Labudović-Borović, Milica
Krstić, Danijela Z.
Čolović, Mirjana B.
Đurić, Dragan M.
Članak u časopisu (Objavljena verzija)
,
© 2018, Springer Science+Business Media, LLC, part of Springer Nature
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The aim of this study was to assess the effects of l-cysteine (Cys) (7 mg/kg) and N-acetyl-l-cysteine (NAC) (50 mg/kg) in the rat liver caused by subchronic i.p. application of methionine (Met) (0.8 mmol/kg) during 21 days. Malondialdehyde (MDA) concentration, glutathione content (GSH), catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), and acetylcholinesterase (AchE) activities were determined in the liver tissue and activities of liver enzymes (AST, ALT, ALP, and GGT) and concentrations of total proteins and albumin were determinated in plasma/serum. Catalase, superoxide dismutase, and acetylcholinesterase activities were increased by Cys and NAC. Met caused periportal mononuclear infiltration and rare focal necrosis of hepatocytes. In Cys- and NAC-supplemented groups, intracellular edema and microvesicular fatty changes without necrosis were noticed. We observed decrease of AST, ALT, and ALP activity in the methionine-treated group. Our results indicate that C...ys and NAC application can increase activity of antioxidative enzymes and prevent intensive histological changes in liver in condition of subchronic methionine exposure.
Ključne reči:
Methionine / Antioxidants / Morphometry / Biochemical analysis / Liver / Rat / Methionine / Antioxidants / Morphometry / Biochemical analysis / Liver / RatIzvor:
Molecular and Cellular Biochemistry, 2018, 448, 1-2, 43-50Finansiranje / projekti:
- Efekti homocisteina i homocisteinu srodnih supstanci na kardiovaskularni sistem: Uloga gasnih transmitera NO, H2S I CO (RS-MESTD-Basic Research (BR or ON)-175043)
DOI: 10.1007/s11010-018-3311-2
ISSN: 0300-8177; 1573-4919
PubMed: 29423685
WoS: 000446385800005
Scopus: 2-s2.0-85041517196
URI
http://link.springer.com/10.1007/s11010-018-3311-2https://vinar.vin.bg.ac.rs/handle/123456789/8060
Kolekcije
Institucija/grupa
VinčaTY - JOUR AU - Stojanović, Marija AU - Todorović, Dajana D. AU - Šćepanović, Ljiljana AU - Mitrović, Dušan M. AU - Borozan, Sunčica Z. AU - Dragutinović, Vesna V. AU - Labudović-Borović, Milica AU - Krstić, Danijela Z. AU - Čolović, Mirjana B. AU - Đurić, Dragan M. PY - 2018 UR - http://link.springer.com/10.1007/s11010-018-3311-2 UR - https://vinar.vin.bg.ac.rs/handle/123456789/8060 AB - The aim of this study was to assess the effects of l-cysteine (Cys) (7 mg/kg) and N-acetyl-l-cysteine (NAC) (50 mg/kg) in the rat liver caused by subchronic i.p. application of methionine (Met) (0.8 mmol/kg) during 21 days. Malondialdehyde (MDA) concentration, glutathione content (GSH), catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), and acetylcholinesterase (AchE) activities were determined in the liver tissue and activities of liver enzymes (AST, ALT, ALP, and GGT) and concentrations of total proteins and albumin were determinated in plasma/serum. Catalase, superoxide dismutase, and acetylcholinesterase activities were increased by Cys and NAC. Met caused periportal mononuclear infiltration and rare focal necrosis of hepatocytes. In Cys- and NAC-supplemented groups, intracellular edema and microvesicular fatty changes without necrosis were noticed. We observed decrease of AST, ALT, and ALP activity in the methionine-treated group. Our results indicate that Cys and NAC application can increase activity of antioxidative enzymes and prevent intensive histological changes in liver in condition of subchronic methionine exposure. T2 - Molecular and Cellular Biochemistry T1 - Subchronic methionine load induces oxidative stress and provokes biochemical and histological changes in the rat liver tissue VL - 448 IS - 1-2 SP - 43 EP - 50 DO - 10.1007/s11010-018-3311-2 ER -
@article{ author = "Stojanović, Marija and Todorović, Dajana D. and Šćepanović, Ljiljana and Mitrović, Dušan M. and Borozan, Sunčica Z. and Dragutinović, Vesna V. and Labudović-Borović, Milica and Krstić, Danijela Z. and Čolović, Mirjana B. and Đurić, Dragan M.", year = "2018", abstract = "The aim of this study was to assess the effects of l-cysteine (Cys) (7 mg/kg) and N-acetyl-l-cysteine (NAC) (50 mg/kg) in the rat liver caused by subchronic i.p. application of methionine (Met) (0.8 mmol/kg) during 21 days. Malondialdehyde (MDA) concentration, glutathione content (GSH), catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), and acetylcholinesterase (AchE) activities were determined in the liver tissue and activities of liver enzymes (AST, ALT, ALP, and GGT) and concentrations of total proteins and albumin were determinated in plasma/serum. Catalase, superoxide dismutase, and acetylcholinesterase activities were increased by Cys and NAC. Met caused periportal mononuclear infiltration and rare focal necrosis of hepatocytes. In Cys- and NAC-supplemented groups, intracellular edema and microvesicular fatty changes without necrosis were noticed. We observed decrease of AST, ALT, and ALP activity in the methionine-treated group. Our results indicate that Cys and NAC application can increase activity of antioxidative enzymes and prevent intensive histological changes in liver in condition of subchronic methionine exposure.", journal = "Molecular and Cellular Biochemistry", title = "Subchronic methionine load induces oxidative stress and provokes biochemical and histological changes in the rat liver tissue", volume = "448", number = "1-2", pages = "43-50", doi = "10.1007/s11010-018-3311-2" }
Stojanović, M., Todorović, D. D., Šćepanović, L., Mitrović, D. M., Borozan, S. Z., Dragutinović, V. V., Labudović-Borović, M., Krstić, D. Z., Čolović, M. B.,& Đurić, D. M.. (2018). Subchronic methionine load induces oxidative stress and provokes biochemical and histological changes in the rat liver tissue. in Molecular and Cellular Biochemistry, 448(1-2), 43-50. https://doi.org/10.1007/s11010-018-3311-2
Stojanović M, Todorović DD, Šćepanović L, Mitrović DM, Borozan SZ, Dragutinović VV, Labudović-Borović M, Krstić DZ, Čolović MB, Đurić DM. Subchronic methionine load induces oxidative stress and provokes biochemical and histological changes in the rat liver tissue. in Molecular and Cellular Biochemistry. 2018;448(1-2):43-50. doi:10.1007/s11010-018-3311-2 .
Stojanović, Marija, Todorović, Dajana D., Šćepanović, Ljiljana, Mitrović, Dušan M., Borozan, Sunčica Z., Dragutinović, Vesna V., Labudović-Borović, Milica, Krstić, Danijela Z., Čolović, Mirjana B., Đurić, Dragan M., "Subchronic methionine load induces oxidative stress and provokes biochemical and histological changes in the rat liver tissue" in Molecular and Cellular Biochemistry, 448, no. 1-2 (2018):43-50, https://doi.org/10.1007/s11010-018-3311-2 . .