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Effects of 17 beta-estradiol on cardiac Na+/K+-ATPase in high fat diet fed rats
dc.creator | Obradović, Milan M. | |
dc.creator | Zafirović, Sonja | |
dc.creator | Jovanović, Aleksandra | |
dc.creator | Sudar, Emina | |
dc.creator | Mousa, Shaker A. | |
dc.creator | Labudović-Borović, Milica | |
dc.creator | Isenović, Esma R. | |
dc.date.accessioned | 2018-03-01T16:30:19Z | |
dc.date.available | 2018-03-01T16:30:19Z | |
dc.date.issued | 2015 | |
dc.identifier.issn | 0303-7207 | |
dc.identifier.uri | https://vinar.vin.bg.ac.rs/handle/123456789/803 | |
dc.description.abstract | The aim of this study was to investigate in vivo effects of estradiol on Na+/K+-ATPase activity/expression in high fat (HF) diet fed rats. Adult male Wistar rats were fed normally (Control, n = 7) or with a HF diet (Obese, n = 14) for 10 weeks. After 10 weeks, half of the obese rats were treated with estradiol (Obese + Estradiol, n = 7, 40 mu g/kg, i.p.) as a bolus injection and 24 h after treatment all the rats were sacrificed. Estradiol in vivo in obese rats in comparison with obese non-treated rats led to a statistically significant increase in concentration of serum Na+ (p LT 0.05), Na+/K+-ATPase activity (p LT 0.01), expression of alpha 1 (p LT 0.01) and alpha 2 (p LT 0.05) subunit of Na+/K+-ATPase, both PI3K subunits p85 (p LT 0.01), p110 (p LT 0.05), and association of IRS-1 with p85 (p LT 0.05), while significantly decrease expression of AT1 (p LT 0.05) and Rho A (p LT 0.01) proteins. Our results suggest that estradiol in vivo in pathophysiological conditions, such as obesity accompanied with insulin resistance stimulates activity and expression of Na+/K+-ATPase by a mechanism that involves the participation of IRS-1/PI3K/Akt signaling. In addition, the decreasing level of AT1 and Rho A proteins estradiol probably attenuates the detrimental effect of obesity to decreased IRS-1/P13K association and consequently reduce Na+/K+-ATPase activity/expression. (C) 2015 Elsevier Ireland Ltd. All rights reserved. | en |
dc.publisher | Elsevier | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173033/RS// | |
dc.rights | restrictedAccess | en |
dc.source | Molecular and Cellular Endocrinology | en |
dc.subject | Estradiol | en |
dc.subject | Sodium potassium adenosine-triphosphatase | en |
dc.subject | Obesity | en |
dc.subject | Insulin resistance | en |
dc.subject | High fat diet | en |
dc.title | Effects of 17 beta-estradiol on cardiac Na+/K+-ATPase in high fat diet fed rats | en |
dc.type | article | en |
dc.rights.license | ARR | |
dcterms.abstract | Моуса, Схакер A.; Обрадовић Милан; Лабудовиц-Боровиц, Милица; Зафировић Соња; Јовановић Aлександра; Судар Емина; Исеновић Есма; | |
dc.citation.volume | 416 | |
dc.citation.issue | C | |
dc.citation.spage | 46 | |
dc.citation.epage | 56 | |
dc.identifier.wos | 000364267800006 | |
dc.identifier.doi | 10.1016/j.mce.2015.08.020 | |
dc.citation.rank | M21 | |
dc.identifier.pmid | 26284496 | |
dc.type.version | publishedVersion | |
dc.identifier.scopus | 2-s2.0-84943625339 |