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dc.creatorObradović, Milan M.
dc.creatorZafirović, Sonja
dc.creatorJovanović, Aleksandra
dc.creatorSudar, Emina
dc.creatorMousa, Shaker A.
dc.creatorLabudović-Borović, Milica
dc.creatorIsenović, Esma R.
dc.date.accessioned2018-03-01T16:30:19Z
dc.date.available2018-03-01T16:30:19Z
dc.date.issued2015
dc.identifier.issn0303-7207 (print)
dc.identifier.urihttp://vinar.vin.bg.ac.rs/handle/123456789/803
dc.description.abstractThe aim of this study was to investigate in vivo effects of estradiol on Na+/K+-ATPase activity/expression in high fat (HF) diet fed rats. Adult male Wistar rats were fed normally (Control, n = 7) or with a HF diet (Obese, n = 14) for 10 weeks. After 10 weeks, half of the obese rats were treated with estradiol (Obese + Estradiol, n = 7, 40 mu g/kg, i.p.) as a bolus injection and 24 h after treatment all the rats were sacrificed. Estradiol in vivo in obese rats in comparison with obese non-treated rats led to a statistically significant increase in concentration of serum Na+ (p LT 0.05), Na+/K+-ATPase activity (p LT 0.01), expression of alpha 1 (p LT 0.01) and alpha 2 (p LT 0.05) subunit of Na+/K+-ATPase, both PI3K subunits p85 (p LT 0.01), p110 (p LT 0.05), and association of IRS-1 with p85 (p LT 0.05), while significantly decrease expression of AT1 (p LT 0.05) and Rho A (p LT 0.01) proteins. Our results suggest that estradiol in vivo in pathophysiological conditions, such as obesity accompanied with insulin resistance stimulates activity and expression of Na+/K+-ATPase by a mechanism that involves the participation of IRS-1/PI3K/Akt signaling. In addition, the decreasing level of AT1 and Rho A proteins estradiol probably attenuates the detrimental effect of obesity to decreased IRS-1/P13K association and consequently reduce Na+/K+-ATPase activity/expression. (C) 2015 Elsevier Ireland Ltd. All rights reserved.en
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173033/RS//
dc.rightsrestrictedAccessen
dc.sourceMolecular and Cellular Endocrinologyen
dc.subjectEstradiolen
dc.subjectSodium potassium adenosine-triphosphataseen
dc.subjectObesityen
dc.subjectInsulin resistanceen
dc.subjectHigh fat dieten
dc.titleEffects of 17 beta-estradiol on cardiac Na+/K+-ATPase in high fat diet fed ratsen
dc.typearticleen
dcterms.abstractМоуса, Схакер A.; Обрадовић Милан; Лабудовиц-Боровиц, Милица; Зафировић Соња; Јовановић Aлександра; Судар Емина; Исеновић Есма;
dc.citation.volume416
dc.citation.issueC
dc.citation.spage46
dc.citation.epage56
dc.identifier.wos000364267800006
dc.identifier.doi10.1016/j.mce.2015.08.020
dc.citation.rankM21
dc.identifier.pmid26284496
dc.identifier.scopus2-s2.0-84943625339


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