Malva sylvestris l. Petals extracts: Composition, antioxidant activity and effect on micronucleus distribution in human lymphocyte
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Kostić, Danijela A.Stanković, Miroslava
Milovanović, Vesna M.
Milovanović, N.
Stojanović, Gordana S.
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© 2017, Scibulcom Ltd
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The contents of phenols, flavonoids anthocyans and antioxidant activity of the water (WE), methanol (ME) and methanol-water (MWE) (50/50, v/v) extracts of M. sylvestris fresh and dry petals were determined. Further, for all above mentioned extracts, in vitro effect of the frequency of micronuclei (MN) on peripheral human lymphocytes was evaluated using cytochalasin-B micronucleus (CBMN) assay. Total phenol content (mg of gallic acid equivalent/g of petals) varied from 13.20 (WE) to 29.20 (ME) of fresh petals and for dry petals from 80 (WE) to 142 (ME). The content of flavonoids (mg of catechin equivalent /g of petals) ranged from 47.50 (WE) to 101.20 (ME) of fresh petals and for dry petals from 439 (WE) to 814 (ME). The anthocyans content (mg of cyanidin-3-O-glucoside/g of petals) ranged from 0.40 (WE) to 0.72 (ME) of fresh petals and for dry petals from 3.13 (WE) to 6.16 (ME). All extracts showed high antioxidant activity (more than 67%). ME of fresh petals showed a greater decrease i...n the frequency of MN (from 23.9 to 29.3%) in all tested concentrations (0.5; 1.0 and 2.0 µg/ml) compared to standard radio of protectant to Amifostine (20.8%). © 2017, Scibulcom Ltd. All rights reserved.
Keywords:
Antioxidant activities / Human lymphocytes / Malva sylvestris L / Micronucleus test / Phenolic compounds compositionSource:
Oxidation Communications, 2017, 40, 2, 602-612Funding / projects:
- Natural products of plants and lichens: isolation, identification, biological activity and application (RS-MESTD-Basic Research (BR or ON)-172047)
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VinčaTY - JOUR AU - Kostić, Danijela A. AU - Stanković, Miroslava AU - Milovanović, Vesna M. AU - Milovanović, N. AU - Stojanović, Gordana S. PY - 2017 UR - https://vinar.vin.bg.ac.rs/handle/123456789/8009 AB - The contents of phenols, flavonoids anthocyans and antioxidant activity of the water (WE), methanol (ME) and methanol-water (MWE) (50/50, v/v) extracts of M. sylvestris fresh and dry petals were determined. Further, for all above mentioned extracts, in vitro effect of the frequency of micronuclei (MN) on peripheral human lymphocytes was evaluated using cytochalasin-B micronucleus (CBMN) assay. Total phenol content (mg of gallic acid equivalent/g of petals) varied from 13.20 (WE) to 29.20 (ME) of fresh petals and for dry petals from 80 (WE) to 142 (ME). The content of flavonoids (mg of catechin equivalent /g of petals) ranged from 47.50 (WE) to 101.20 (ME) of fresh petals and for dry petals from 439 (WE) to 814 (ME). The anthocyans content (mg of cyanidin-3-O-glucoside/g of petals) ranged from 0.40 (WE) to 0.72 (ME) of fresh petals and for dry petals from 3.13 (WE) to 6.16 (ME). All extracts showed high antioxidant activity (more than 67%). ME of fresh petals showed a greater decrease in the frequency of MN (from 23.9 to 29.3%) in all tested concentrations (0.5; 1.0 and 2.0 µg/ml) compared to standard radio of protectant to Amifostine (20.8%). © 2017, Scibulcom Ltd. All rights reserved. T2 - Oxidation Communications T1 - Malva sylvestris l. Petals extracts: Composition, antioxidant activity and effect on micronucleus distribution in human lymphocyte VL - 40 IS - 2 SP - 602 EP - 612 UR - https://hdl.handle.net/21.15107/rcub_vinar_8009 ER -
@article{ author = "Kostić, Danijela A. and Stanković, Miroslava and Milovanović, Vesna M. and Milovanović, N. and Stojanović, Gordana S.", year = "2017", abstract = "The contents of phenols, flavonoids anthocyans and antioxidant activity of the water (WE), methanol (ME) and methanol-water (MWE) (50/50, v/v) extracts of M. sylvestris fresh and dry petals were determined. Further, for all above mentioned extracts, in vitro effect of the frequency of micronuclei (MN) on peripheral human lymphocytes was evaluated using cytochalasin-B micronucleus (CBMN) assay. Total phenol content (mg of gallic acid equivalent/g of petals) varied from 13.20 (WE) to 29.20 (ME) of fresh petals and for dry petals from 80 (WE) to 142 (ME). The content of flavonoids (mg of catechin equivalent /g of petals) ranged from 47.50 (WE) to 101.20 (ME) of fresh petals and for dry petals from 439 (WE) to 814 (ME). The anthocyans content (mg of cyanidin-3-O-glucoside/g of petals) ranged from 0.40 (WE) to 0.72 (ME) of fresh petals and for dry petals from 3.13 (WE) to 6.16 (ME). All extracts showed high antioxidant activity (more than 67%). ME of fresh petals showed a greater decrease in the frequency of MN (from 23.9 to 29.3%) in all tested concentrations (0.5; 1.0 and 2.0 µg/ml) compared to standard radio of protectant to Amifostine (20.8%). © 2017, Scibulcom Ltd. All rights reserved.", journal = "Oxidation Communications", title = "Malva sylvestris l. Petals extracts: Composition, antioxidant activity and effect on micronucleus distribution in human lymphocyte", volume = "40", number = "2", pages = "602-612", url = "https://hdl.handle.net/21.15107/rcub_vinar_8009" }
Kostić, D. A., Stanković, M., Milovanović, V. M., Milovanović, N.,& Stojanović, G. S.. (2017). Malva sylvestris l. Petals extracts: Composition, antioxidant activity and effect on micronucleus distribution in human lymphocyte. in Oxidation Communications, 40(2), 602-612. https://hdl.handle.net/21.15107/rcub_vinar_8009
Kostić DA, Stanković M, Milovanović VM, Milovanović N, Stojanović GS. Malva sylvestris l. Petals extracts: Composition, antioxidant activity and effect on micronucleus distribution in human lymphocyte. in Oxidation Communications. 2017;40(2):602-612. https://hdl.handle.net/21.15107/rcub_vinar_8009 .
Kostić, Danijela A., Stanković, Miroslava, Milovanović, Vesna M., Milovanović, N., Stojanović, Gordana S., "Malva sylvestris l. Petals extracts: Composition, antioxidant activity and effect on micronucleus distribution in human lymphocyte" in Oxidation Communications, 40, no. 2 (2017):602-612, https://hdl.handle.net/21.15107/rcub_vinar_8009 .