Methylation status of p16 and p14 genes in locally advanced rectal cancer: Potential clinical implication
Authors
Kožik, BojanaKokanov, Nikola
Knežević-Ušaj, Slavica
Nikolić, Ivan
Davidović, Radoslav S.
Jovanović-Ćupić, Snežana P.
Krajnović, Milena M.
Article (Published version)
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Methylation of p16 and p14 genes is a common event in colorectal cancers; however, their exact role in the prediction of patients' outcome is unclear. We conducted this retrospective study to evaluate their potential predictive and/or prognostic roles. Methylation-specific PCR was used to examine the methylation status of p16 and p14 in pretherapeutic and preoperative biopsy specimens of 60 patients with locally advanced rectal cancer. The methylation status of the examined genes did not affect the response to preoperative chemoradiotherapy (CRT), recurrence rate and overall survival. However, patients with a simultaneous presence of either p16 or p14 methylation and high vascular endothelial growth factor (VEGF) expression showed a significantly worse response to CRT (p=0.005 and p=0.038, respectively). Moreover, patients with both p16 methylation and high VEGF expression had significantly shorter overall survival (p=0.010), while no such association was found in patients with p14 met...hylation and high VEGF expression. On the other hand, a subgroup of patients with p16 methylation and low VEGF and high epidermal growth factor receptor (EGFR) expression showed a significantly better response to CRT (p=0.024). The obtained results point to the importance of p16 and p14 methylation analyses in combination with VEGF and EGFR expression, aimed at better predicting treatment response and patient outcome. © 2018 by the Serbian Biological Society.
Keywords:
rectal cancer / p16 / p14 / DNA methylation / biomarkers / VEGF / EGFRSource:
Archives of Biological Sciences, 2018, 70, 4, 681-690Funding / projects:
- Molecular determinants for tumor marker design (RS-173049)
DOI: 10.2298/ABS180316030K
ISSN: 0354-4664; 1821-4339
WoS: 000452187000010
Scopus: 2-s2.0-85058812536
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http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46641800030Khttps://vinar.vin.bg.ac.rs/handle/123456789/8002
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VinčaTY - JOUR AU - Kožik, Bojana AU - Kokanov, Nikola AU - Knežević-Ušaj, Slavica AU - Nikolić, Ivan AU - Davidović, Radoslav S. AU - Jovanović-Ćupić, Snežana P. AU - Krajnović, Milena M. PY - 2018 UR - http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46641800030K UR - https://vinar.vin.bg.ac.rs/handle/123456789/8002 AB - Methylation of p16 and p14 genes is a common event in colorectal cancers; however, their exact role in the prediction of patients' outcome is unclear. We conducted this retrospective study to evaluate their potential predictive and/or prognostic roles. Methylation-specific PCR was used to examine the methylation status of p16 and p14 in pretherapeutic and preoperative biopsy specimens of 60 patients with locally advanced rectal cancer. The methylation status of the examined genes did not affect the response to preoperative chemoradiotherapy (CRT), recurrence rate and overall survival. However, patients with a simultaneous presence of either p16 or p14 methylation and high vascular endothelial growth factor (VEGF) expression showed a significantly worse response to CRT (p=0.005 and p=0.038, respectively). Moreover, patients with both p16 methylation and high VEGF expression had significantly shorter overall survival (p=0.010), while no such association was found in patients with p14 methylation and high VEGF expression. On the other hand, a subgroup of patients with p16 methylation and low VEGF and high epidermal growth factor receptor (EGFR) expression showed a significantly better response to CRT (p=0.024). The obtained results point to the importance of p16 and p14 methylation analyses in combination with VEGF and EGFR expression, aimed at better predicting treatment response and patient outcome. © 2018 by the Serbian Biological Society. T2 - Archives of Biological Sciences T1 - Methylation status of p16 and p14 genes in locally advanced rectal cancer: Potential clinical implication VL - 70 IS - 4 SP - 681 EP - 690 DO - 10.2298/ABS180316030K ER -
@article{ author = "Kožik, Bojana and Kokanov, Nikola and Knežević-Ušaj, Slavica and Nikolić, Ivan and Davidović, Radoslav S. and Jovanović-Ćupić, Snežana P. and Krajnović, Milena M.", year = "2018", abstract = "Methylation of p16 and p14 genes is a common event in colorectal cancers; however, their exact role in the prediction of patients' outcome is unclear. We conducted this retrospective study to evaluate their potential predictive and/or prognostic roles. Methylation-specific PCR was used to examine the methylation status of p16 and p14 in pretherapeutic and preoperative biopsy specimens of 60 patients with locally advanced rectal cancer. The methylation status of the examined genes did not affect the response to preoperative chemoradiotherapy (CRT), recurrence rate and overall survival. However, patients with a simultaneous presence of either p16 or p14 methylation and high vascular endothelial growth factor (VEGF) expression showed a significantly worse response to CRT (p=0.005 and p=0.038, respectively). Moreover, patients with both p16 methylation and high VEGF expression had significantly shorter overall survival (p=0.010), while no such association was found in patients with p14 methylation and high VEGF expression. On the other hand, a subgroup of patients with p16 methylation and low VEGF and high epidermal growth factor receptor (EGFR) expression showed a significantly better response to CRT (p=0.024). The obtained results point to the importance of p16 and p14 methylation analyses in combination with VEGF and EGFR expression, aimed at better predicting treatment response and patient outcome. © 2018 by the Serbian Biological Society.", journal = "Archives of Biological Sciences", title = "Methylation status of p16 and p14 genes in locally advanced rectal cancer: Potential clinical implication", volume = "70", number = "4", pages = "681-690", doi = "10.2298/ABS180316030K" }
Kožik, B., Kokanov, N., Knežević-Ušaj, S., Nikolić, I., Davidović, R. S., Jovanović-Ćupić, S. P.,& Krajnović, M. M.. (2018). Methylation status of p16 and p14 genes in locally advanced rectal cancer: Potential clinical implication. in Archives of Biological Sciences, 70(4), 681-690. https://doi.org/10.2298/ABS180316030K
Kožik B, Kokanov N, Knežević-Ušaj S, Nikolić I, Davidović RS, Jovanović-Ćupić SP, Krajnović MM. Methylation status of p16 and p14 genes in locally advanced rectal cancer: Potential clinical implication. in Archives of Biological Sciences. 2018;70(4):681-690. doi:10.2298/ABS180316030K .
Kožik, Bojana, Kokanov, Nikola, Knežević-Ušaj, Slavica, Nikolić, Ivan, Davidović, Radoslav S., Jovanović-Ćupić, Snežana P., Krajnović, Milena M., "Methylation status of p16 and p14 genes in locally advanced rectal cancer: Potential clinical implication" in Archives of Biological Sciences, 70, no. 4 (2018):681-690, https://doi.org/10.2298/ABS180316030K . .