Adsorption of Candida rugosa lipase onto alumina: effect of surface charge
2015
Autori
Izrael-Živković, Lidija T.Živković, Ljiljana
Jokić, Bojan M.
Savić, Andrija B.
Karadzic, Ivanka M.
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The impact of the surface charge of alumina supports on the adsorption of Candida rugosa lipase was investigated in terms of the zeta potentials of the adsorption partners. The lipase adhered onto alumina with similar efficiency under both repulsive and attractive electrostatic conditions, shifting the zeta potential of the support towards that of the enzyme. The behavior was explained by a heterogeneous distribution of the surface charge of the lipase molecule. Special emphasis in this study was placed on the effect of immobilization on the enzyme kinetics and principal reasons for enzyme immobilization: improvement in stability and potential for reuse. The enzyme affinity was not altered by its adsorption onto alumina, while the Vmax value of the lipase decreased. The thermostability of the adsorbed lipase was improved. A significant potential for reuse was found.
Ključne reči:
alumina / lipase / Adsorption / immobilization / Candida rugosaIzvor:
Journal of the Serbian Chemical Society, 2015, 80, 9, 1113-1125Finansiranje / projekti:
- Sinteza, procesiranje i karakterizacija nanostrukturnih materijala za primenu u oblasti energije, mehaničkog inženjerstva, zaštite životne stredine i biomedicine (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-45012)
- Simultana bioremedijacija i soilifikacija degradiranih prostora, za očuvanje prirodnih resursa biološki aktivnih supstanci i razvoj i proizvodnju biomaterijala i dijetetskih proizvoda (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-43004)
DOI: 10.2298/JSC150222035I
ISSN: 0352-5139
WoS: 000364205600002
Scopus: 2-s2.0-84947609900
Kolekcije
Institucija/grupa
VinčaTY - JOUR AU - Izrael-Živković, Lidija T. AU - Živković, Ljiljana AU - Jokić, Bojan M. AU - Savić, Andrija B. AU - Karadzic, Ivanka M. PY - 2015 UR - https://vinar.vin.bg.ac.rs/handle/123456789/796 AB - The impact of the surface charge of alumina supports on the adsorption of Candida rugosa lipase was investigated in terms of the zeta potentials of the adsorption partners. The lipase adhered onto alumina with similar efficiency under both repulsive and attractive electrostatic conditions, shifting the zeta potential of the support towards that of the enzyme. The behavior was explained by a heterogeneous distribution of the surface charge of the lipase molecule. Special emphasis in this study was placed on the effect of immobilization on the enzyme kinetics and principal reasons for enzyme immobilization: improvement in stability and potential for reuse. The enzyme affinity was not altered by its adsorption onto alumina, while the Vmax value of the lipase decreased. The thermostability of the adsorbed lipase was improved. A significant potential for reuse was found. T2 - Journal of the Serbian Chemical Society T1 - Adsorption of Candida rugosa lipase onto alumina: effect of surface charge VL - 80 IS - 9 SP - 1113 EP - 1125 DO - 10.2298/JSC150222035I ER -
@article{ author = "Izrael-Živković, Lidija T. and Živković, Ljiljana and Jokić, Bojan M. and Savić, Andrija B. and Karadzic, Ivanka M.", year = "2015", abstract = "The impact of the surface charge of alumina supports on the adsorption of Candida rugosa lipase was investigated in terms of the zeta potentials of the adsorption partners. The lipase adhered onto alumina with similar efficiency under both repulsive and attractive electrostatic conditions, shifting the zeta potential of the support towards that of the enzyme. The behavior was explained by a heterogeneous distribution of the surface charge of the lipase molecule. Special emphasis in this study was placed on the effect of immobilization on the enzyme kinetics and principal reasons for enzyme immobilization: improvement in stability and potential for reuse. The enzyme affinity was not altered by its adsorption onto alumina, while the Vmax value of the lipase decreased. The thermostability of the adsorbed lipase was improved. A significant potential for reuse was found.", journal = "Journal of the Serbian Chemical Society", title = "Adsorption of Candida rugosa lipase onto alumina: effect of surface charge", volume = "80", number = "9", pages = "1113-1125", doi = "10.2298/JSC150222035I" }
Izrael-Živković, L. T., Živković, L., Jokić, B. M., Savić, A. B.,& Karadzic, I. M.. (2015). Adsorption of Candida rugosa lipase onto alumina: effect of surface charge. in Journal of the Serbian Chemical Society, 80(9), 1113-1125. https://doi.org/10.2298/JSC150222035I
Izrael-Živković LT, Živković L, Jokić BM, Savić AB, Karadzic IM. Adsorption of Candida rugosa lipase onto alumina: effect of surface charge. in Journal of the Serbian Chemical Society. 2015;80(9):1113-1125. doi:10.2298/JSC150222035I .
Izrael-Živković, Lidija T., Živković, Ljiljana, Jokić, Bojan M., Savić, Andrija B., Karadzic, Ivanka M., "Adsorption of Candida rugosa lipase onto alumina: effect of surface charge" in Journal of the Serbian Chemical Society, 80, no. 9 (2015):1113-1125, https://doi.org/10.2298/JSC150222035I . .