Modulation of rat synaptosomal ATPases and acetylcholinesterase activities induced by chronic exposure to the static magnetic field
Krstić, Danijela Z.
Čolović, Mirjana B.
Nešović Ostojić, Jelena
De Luka, Silvio R.
Đorđević, Drago M.
Članak u časopisu (Objavljena verzija)
© 2018 Taylor & Francis Group, LLC
MetapodaciPrikaz svih podataka o dokumentu
Purpose: It is considered that exposure to static magnetic fields (SMF) may have both detrimental and therapeutic effect, but the mechanism of SMF influence on the living organisms is not well understood. Since the adenosine triphosphatases (ATPases) and acetylcholinesterase (AChE) are involved in both physiological and pathological processes, the modulation of Na+/K+-ATPase, ecto-ATPases and AChE activities, as well as oxidative stress responses were followed in synaptosomes isolated from rats after chronic exposure toward differently oriented SMF. Material and methods: Wistar albino rats were randomly divided into three experimental groups (six animals per group): Up and Down group - exposed to upward and downward oriented SMF, respectively, and Control group. After 50 days, the rats were sacrificed, and synaptosomes were isolated from the whole rat brain and used for testing the enzyme activities and oxidative stress parameters. Results: Chronic exposure to 1 mT SMF significantly in...creased ATPases, AChE activities, and malondialdehyde (MDA) level in both exposed groups, compared to control values. The significant decrease in synaptosomal catalase activity (1.48 ± 0.17 U/mg protein) induced by exposure to the downward oriented field, compared to those obtained for Control group (2.60 ± 0.29 U/mg protein), and Up group (2.72 ± 0.21 U/mg protein). Conclusions: It could be concluded that chronic exposure to differently oriented SMF increases ATPases and AChE activities in rat synaptosomes. Since brain ATPases and AChE have important roles in the pathogenesis of several neurological diseases, SMF influence on the activity of these enzymes may have potential therapeutic importance. © 2018, Copyright © 2018 Taylor & Francis Group, LLC.
Ključne reči:Static magnetic field / ATPases / acetylcholinesterase / malondialdehyde / catalase / hydroperoxidases
Izvor:International Journal of Radiation Biology, 2018, 94, 11, 1062-1071
- Značaj rane dijagnostike sindroma opstruktivne apneje u spavanju kod vozača profesionalaca koji upravljaju motornim vozilima (RS-175081)
- Istraživanja interakcija enzima sa toksičnim i farmakološki aktivnim molekulima (RS-172023)
ISSN: 0955-3002 (print); 1362-3095 (electronic)