Modulation of rat synaptosomal ATPases and acetylcholinesterase activities induced by chronic exposure to the static magnetic field
Само за регистроване кориснике
2018
Аутори
Dinčić, MarkoKrstić, Danijela Z.
Čolović, Mirjana B.
Nešović Ostojić, Jelena
Kovačević, Sanjin
De Luka, Silvio R.
Đorđević, Drago M.
Ćirković, Saša
Brkić, Predrag
Todorović, Jasna
Чланак у часопису (Објављена верзија)
,
© 2018 Taylor & Francis Group, LLC
Метаподаци
Приказ свих података о документуАпстракт
Purpose: It is considered that exposure to static magnetic fields (SMF) may have both detrimental and therapeutic effect, but the mechanism of SMF influence on the living organisms is not well understood. Since the adenosine triphosphatases (ATPases) and acetylcholinesterase (AChE) are involved in both physiological and pathological processes, the modulation of Na+/K+-ATPase, ecto-ATPases and AChE activities, as well as oxidative stress responses were followed in synaptosomes isolated from rats after chronic exposure toward differently oriented SMF. Material and methods: Wistar albino rats were randomly divided into three experimental groups (six animals per group): Up and Down group - exposed to upward and downward oriented SMF, respectively, and Control group. After 50 days, the rats were sacrificed, and synaptosomes were isolated from the whole rat brain and used for testing the enzyme activities and oxidative stress parameters. Results: Chronic exposure to 1 mT SMF significantly in...creased ATPases, AChE activities, and malondialdehyde (MDA) level in both exposed groups, compared to control values. The significant decrease in synaptosomal catalase activity (1.48 ± 0.17 U/mg protein) induced by exposure to the downward oriented field, compared to those obtained for Control group (2.60 ± 0.29 U/mg protein), and Up group (2.72 ± 0.21 U/mg protein). Conclusions: It could be concluded that chronic exposure to differently oriented SMF increases ATPases and AChE activities in rat synaptosomes. Since brain ATPases and AChE have important roles in the pathogenesis of several neurological diseases, SMF influence on the activity of these enzymes may have potential therapeutic importance. © 2018, Copyright © 2018 Taylor & Francis Group, LLC.
Кључне речи:
Static magnetic field / ATPases / acetylcholinesterase / malondialdehyde / catalase / hydroperoxidasesИзвор:
International Journal of Radiation Biology, 2018, 94, 11, 1062-1071Финансирање / пројекти:
- Значај ране дијагностике синдрома опструктивне апнеје у спавању код возача професионалаца који управљају моторним возилима (RS-175081)
- Истраживања интеракција ензима са токсичним и фармаколошки активним молекулима (RS-172023)
DOI: 10.1080/09553002.2018.1518611
ISSN: 0955-3002; 1362-3095
PubMed: 30238840
WoS: 000451598500013
Scopus: 2-s2.0-85053534681
URI
https://www.tandfonline.com/doi/full/10.1080/09553002.2018.1518611https://vinar.vin.bg.ac.rs/handle/123456789/7957
Колекције
Институција/група
VinčaTY - JOUR AU - Dinčić, Marko AU - Krstić, Danijela Z. AU - Čolović, Mirjana B. AU - Nešović Ostojić, Jelena AU - Kovačević, Sanjin AU - De Luka, Silvio R. AU - Đorđević, Drago M. AU - Ćirković, Saša AU - Brkić, Predrag AU - Todorović, Jasna PY - 2018 UR - https://www.tandfonline.com/doi/full/10.1080/09553002.2018.1518611 UR - https://vinar.vin.bg.ac.rs/handle/123456789/7957 AB - Purpose: It is considered that exposure to static magnetic fields (SMF) may have both detrimental and therapeutic effect, but the mechanism of SMF influence on the living organisms is not well understood. Since the adenosine triphosphatases (ATPases) and acetylcholinesterase (AChE) are involved in both physiological and pathological processes, the modulation of Na+/K+-ATPase, ecto-ATPases and AChE activities, as well as oxidative stress responses were followed in synaptosomes isolated from rats after chronic exposure toward differently oriented SMF. Material and methods: Wistar albino rats were randomly divided into three experimental groups (six animals per group): Up and Down group - exposed to upward and downward oriented SMF, respectively, and Control group. After 50 days, the rats were sacrificed, and synaptosomes were isolated from the whole rat brain and used for testing the enzyme activities and oxidative stress parameters. Results: Chronic exposure to 1 mT SMF significantly increased ATPases, AChE activities, and malondialdehyde (MDA) level in both exposed groups, compared to control values. The significant decrease in synaptosomal catalase activity (1.48 ± 0.17 U/mg protein) induced by exposure to the downward oriented field, compared to those obtained for Control group (2.60 ± 0.29 U/mg protein), and Up group (2.72 ± 0.21 U/mg protein). Conclusions: It could be concluded that chronic exposure to differently oriented SMF increases ATPases and AChE activities in rat synaptosomes. Since brain ATPases and AChE have important roles in the pathogenesis of several neurological diseases, SMF influence on the activity of these enzymes may have potential therapeutic importance. © 2018, Copyright © 2018 Taylor & Francis Group, LLC. T2 - International Journal of Radiation Biology T1 - Modulation of rat synaptosomal ATPases and acetylcholinesterase activities induced by chronic exposure to the static magnetic field VL - 94 IS - 11 SP - 1062 EP - 1071 DO - 10.1080/09553002.2018.1518611 ER -
@article{ author = "Dinčić, Marko and Krstić, Danijela Z. and Čolović, Mirjana B. and Nešović Ostojić, Jelena and Kovačević, Sanjin and De Luka, Silvio R. and Đorđević, Drago M. and Ćirković, Saša and Brkić, Predrag and Todorović, Jasna", year = "2018", abstract = "Purpose: It is considered that exposure to static magnetic fields (SMF) may have both detrimental and therapeutic effect, but the mechanism of SMF influence on the living organisms is not well understood. Since the adenosine triphosphatases (ATPases) and acetylcholinesterase (AChE) are involved in both physiological and pathological processes, the modulation of Na+/K+-ATPase, ecto-ATPases and AChE activities, as well as oxidative stress responses were followed in synaptosomes isolated from rats after chronic exposure toward differently oriented SMF. Material and methods: Wistar albino rats were randomly divided into three experimental groups (six animals per group): Up and Down group - exposed to upward and downward oriented SMF, respectively, and Control group. After 50 days, the rats were sacrificed, and synaptosomes were isolated from the whole rat brain and used for testing the enzyme activities and oxidative stress parameters. Results: Chronic exposure to 1 mT SMF significantly increased ATPases, AChE activities, and malondialdehyde (MDA) level in both exposed groups, compared to control values. The significant decrease in synaptosomal catalase activity (1.48 ± 0.17 U/mg protein) induced by exposure to the downward oriented field, compared to those obtained for Control group (2.60 ± 0.29 U/mg protein), and Up group (2.72 ± 0.21 U/mg protein). Conclusions: It could be concluded that chronic exposure to differently oriented SMF increases ATPases and AChE activities in rat synaptosomes. Since brain ATPases and AChE have important roles in the pathogenesis of several neurological diseases, SMF influence on the activity of these enzymes may have potential therapeutic importance. © 2018, Copyright © 2018 Taylor & Francis Group, LLC.", journal = "International Journal of Radiation Biology", title = "Modulation of rat synaptosomal ATPases and acetylcholinesterase activities induced by chronic exposure to the static magnetic field", volume = "94", number = "11", pages = "1062-1071", doi = "10.1080/09553002.2018.1518611" }
Dinčić, M., Krstić, D. Z., Čolović, M. B., Nešović Ostojić, J., Kovačević, S., De Luka, S. R., Đorđević, D. M., Ćirković, S., Brkić, P.,& Todorović, J.. (2018). Modulation of rat synaptosomal ATPases and acetylcholinesterase activities induced by chronic exposure to the static magnetic field. in International Journal of Radiation Biology, 94(11), 1062-1071. https://doi.org/10.1080/09553002.2018.1518611
Dinčić M, Krstić DZ, Čolović MB, Nešović Ostojić J, Kovačević S, De Luka SR, Đorđević DM, Ćirković S, Brkić P, Todorović J. Modulation of rat synaptosomal ATPases and acetylcholinesterase activities induced by chronic exposure to the static magnetic field. in International Journal of Radiation Biology. 2018;94(11):1062-1071. doi:10.1080/09553002.2018.1518611 .
Dinčić, Marko, Krstić, Danijela Z., Čolović, Mirjana B., Nešović Ostojić, Jelena, Kovačević, Sanjin, De Luka, Silvio R., Đorđević, Drago M., Ćirković, Saša, Brkić, Predrag, Todorović, Jasna, "Modulation of rat synaptosomal ATPases and acetylcholinesterase activities induced by chronic exposure to the static magnetic field" in International Journal of Radiation Biology, 94, no. 11 (2018):1062-1071, https://doi.org/10.1080/09553002.2018.1518611 . .