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dc.creatorŽivotić, Ivan
dc.creatorĐurić, Tamara
dc.creatorStanković, Aleksandra
dc.creatorStanković, Goran
dc.creatorMilašinović, Dejan
dc.creatorDekleva, Milica
dc.creatorMarković-Nikolić, Nataša
dc.creatorAlavantić, Dragan
dc.creatorŽivković, Maja
dc.date.accessioned2018-11-23T08:43:13Z
dc.date.available2018-11-23T08:43:13Z
dc.date.issued2018
dc.identifier.issn0021-9150
dc.identifier.issn1879-1484
dc.identifier.urihttps://linkinghub.elsevier.com/retrieve/pii/S0021915018309699
dc.identifier.urihttps://vinar.vin.bg.ac.rs/handle/123456789/7953
dc.description.abstractAim: Myocardial infarction (MI) incidence is still a major burden in the terms of premature death and disability world-wide. Genetic studies up to date explained a limited portion of disease inheritance. Recently, the DT haplotype of variants rs36212560 I/D (insertion/deletion) and 2275888 T/C in HACD gene (9p21.3) have been significantly associated with the risk of carotid plaque occurrence among males. Methods: We aimed to validate these results by investigating 518 MI patients (366 males) and 303 (167 males) healthy controls from Serbia. Also, he HACD4 gene expression analysis has been conducted in the peripheral blood mononuclear cells of 72 MI patients, 6 months after the MI. Results were obtained using ABI-Prism 7500 RT-PCR for rs2275888 allelic discrimination and HACD4 mRNA relative quantitation. PCR and acrylamide gel electrophoresis were used to distinguish 5 base pairs rs36212560 insertion deletion polymorphisms. Results: Haplotype analysis (using Thesias software) showed that DT haplotype carriers had significantly higher risk for MI (OR ¼ 1.42, CI 1.08- 1.85, p¼0.01) compared with most frequent IT haplotype. In gender separated groups association remained significant only among males (OR ¼ 1.76, 1.266-2.46, p¼0.0008). Results were adjusted for MI traditional risk factors (Age, BMI, HDLC, LDLC, Tg, hypertension and smoking). Investigated genetic variants were not associated with HACD4 expression. Significant correlation was found between HACD4 mRNA level and age (r¼0.36, p¼0.001). Conclusions: We have shown that rs36212560 and rs2275888 DT haplotype from HACD4 gene is significantly and independently associated with the MI occurrence in males. Additional studies are needed to confirm these results.en
dc.rightsrestrictedAccess
dc.sourceAtherosclerosis
dc.titleHACD4 haplotype confers risk of myocardial infarction among males in the population of Serbiaen
dc.typeconferenceObjecten
dc.rights.licenseARR
dcterms.abstractДеклева, Милица; Животић, Иван; Ђурић, Тамара; Станковић, Aлександра; Станковић, Горан; Милашиновић, Дејан; Марковић-Николић, Наташа; Aлавантић, Драган; Живковић, Маја;
dc.citation.volume275
dc.citation.spagee210
dc.citation.epagee211
dc.identifier.wos000442512600669
dc.identifier.doi10.1016/j.atherosclerosis.2018.06.657
dc.description.other86th Congress of the European-Atherosclerosis-Society (EAS), Lisbon,PORTUGAL, MAY 05-08, 2018en
dc.type.versionpublishedVersion


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