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dc.creatorVukojević, Vesna
dc.creatorĐurđić, Slađana
dc.creatorŠvorc, Lubomir
dc.creatorĆirković Veličković, Tanja
dc.creatorMutić, Jelena J.
dc.creatorStanković, Dalibor M.
dc.date.accessioned2018-11-02T10:58:17Z
dc.date.available2018-11-02T10:58:17Z
dc.date.issued2018
dc.identifier.issn1936-9751 (print)
dc.identifier.issn1936-976X (electronic)
dc.identifier.urihttp://link.springer.com/10.1007/s12161-018-1249-3
dc.identifier.urihttp://vinar.vin.bg.ac.rs/handle/123456789/7919
dc.description.abstractErgosterol (Erg) is the major sterol component in the plasma membranes of fungi. The main goal of this work was to develop rapid, reliable, and cost-effective method toward Erg detection based on application of unmodified boron-doped diamond electrode. Several organic solvents were tested (acetonitrile, dimethyformamide, dimethylsulfoxide, dichloromethane), and it was found that best analytical characteristics were achieved using acetonitrile containing 0.1 M tetrabutylammonium hexa-fluorophosphate as supporting electrolyte. Oval shaped and well-defined oxidation peak was recorded at potential of around 1.17 V versus Ag/Ag+ non-aqueous reference electrode. Under optimized experimental conditions, linear working range from 2 to 200 mu M was estimated, with limit of detection of 0.7 mu M, using square wave voltammetry. Negligible effect of possible interfering compound was observed. Proposed methodology was successfully applied for estimation of Erg content in mushrooms extract with satisfactory results in comparison with spectrophotometric procedure. Obtained results clearly show that developed analytical methodology can be adequate replacement for the, up to date, used methods and open-novel approach for ergosterol detection.
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/621375/EU//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172030/RS//
dc.relationGrant Agency of the Slovak Republic (1/0489/16)
dc.rightsrestrictedAccess
dc.sourceFood Analytical Methods
dc.subjectErgosterolen
dc.subjectBoron-doped diamond electrodeen
dc.subjectMushroomsen
dc.titleAnalytical Approach for Detection of Ergosterol in Mushrooms Based on Modification Free Electrochemical Sensor in Organic Solventsen
dc.typearticleen
dc.rights.licenseARR
dcterms.abstractЋирковић Величковић, Тања; Мутић, Јелена Ј.; Станковић, Далибор М.; Вукојевић, Весна; Ђурђић, Слађана; Шворц, Лубомир;
dc.rights.holder© 2018, Springer Science+Business Media, LLC, part of Springer Nature
dc.citation.volume11
dc.citation.issue9
dc.citation.spage2590
dc.citation.epage2596
dc.identifier.wos000438995800026
dc.identifier.doi10.1007/s12161-018-1249-3
dc.type.versionpublishedVersion
dc.identifier.scopus2-s2.0-85045073042


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