Analytical Approach for Detection of Ergosterol in Mushrooms Based on Modification Free Electrochemical Sensor in Organic Solvents
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2018
Authors
Vukojević, VesnaĐurđić, Slađana
Švorc, Lubomir
Ćirković Veličković, Tanja
Mutić, Jelena J.
Stanković, Dalibor M.
Article (Published version)
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© 2018, Springer Science+Business Media, LLC, part of Springer Nature
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Ergosterol (Erg) is the major sterol component in the plasma membranes of fungi. The main goal of this work was to develop rapid, reliable, and cost-effective method toward Erg detection based on application of unmodified boron-doped diamond electrode. Several organic solvents were tested (acetonitrile, dimethyformamide, dimethylsulfoxide, dichloromethane), and it was found that best analytical characteristics were achieved using acetonitrile containing 0.1 M tetrabutylammonium hexa-fluorophosphate as supporting electrolyte. Oval shaped and well-defined oxidation peak was recorded at potential of around 1.17 V versus Ag/Ag+ non-aqueous reference electrode. Under optimized experimental conditions, linear working range from 2 to 200 mu M was estimated, with limit of detection of 0.7 mu M, using square wave voltammetry. Negligible effect of possible interfering compound was observed. Proposed methodology was successfully applied for estimation of Erg content in mushrooms extract with sati...sfactory results in comparison with spectrophotometric procedure. Obtained results clearly show that developed analytical methodology can be adequate replacement for the, up to date, used methods and open-novel approach for ergosterol detection.
Keywords:
Ergosterol / Boron-doped diamond electrode / MushroomsSource:
Food Analytical Methods, 2018, 11, 9, 2590-2596Funding / projects:
- Strengthening of the MagBioVin Research and Innovation Team for Development of Novel Approaches for Tumour Therapy based on Nanostructured Materials (EU-FP7-621375)
- Application of advanced oxidation processes and nanostructured oxide materials for the removal of pollutants from the environment, development and optimisation of instrumental techniques for efficiency monitoring (RS-MESTD-Basic Research (BR or ON)-172030)
- Grant Agency of the Slovak Republic (1/0489/16)
DOI: 10.1007/s12161-018-1249-3
ISSN: 1936-9751; 1936-976X
WoS: 000438995800026
Scopus: 2-s2.0-85045073042
URI
http://link.springer.com/10.1007/s12161-018-1249-3https://vinar.vin.bg.ac.rs/handle/123456789/7919
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VinčaTY - JOUR AU - Vukojević, Vesna AU - Đurđić, Slađana AU - Švorc, Lubomir AU - Ćirković Veličković, Tanja AU - Mutić, Jelena J. AU - Stanković, Dalibor M. PY - 2018 UR - http://link.springer.com/10.1007/s12161-018-1249-3 UR - https://vinar.vin.bg.ac.rs/handle/123456789/7919 AB - Ergosterol (Erg) is the major sterol component in the plasma membranes of fungi. The main goal of this work was to develop rapid, reliable, and cost-effective method toward Erg detection based on application of unmodified boron-doped diamond electrode. Several organic solvents were tested (acetonitrile, dimethyformamide, dimethylsulfoxide, dichloromethane), and it was found that best analytical characteristics were achieved using acetonitrile containing 0.1 M tetrabutylammonium hexa-fluorophosphate as supporting electrolyte. Oval shaped and well-defined oxidation peak was recorded at potential of around 1.17 V versus Ag/Ag+ non-aqueous reference electrode. Under optimized experimental conditions, linear working range from 2 to 200 mu M was estimated, with limit of detection of 0.7 mu M, using square wave voltammetry. Negligible effect of possible interfering compound was observed. Proposed methodology was successfully applied for estimation of Erg content in mushrooms extract with satisfactory results in comparison with spectrophotometric procedure. Obtained results clearly show that developed analytical methodology can be adequate replacement for the, up to date, used methods and open-novel approach for ergosterol detection. T2 - Food Analytical Methods T1 - Analytical Approach for Detection of Ergosterol in Mushrooms Based on Modification Free Electrochemical Sensor in Organic Solvents VL - 11 IS - 9 SP - 2590 EP - 2596 DO - 10.1007/s12161-018-1249-3 ER -
@article{ author = "Vukojević, Vesna and Đurđić, Slađana and Švorc, Lubomir and Ćirković Veličković, Tanja and Mutić, Jelena J. and Stanković, Dalibor M.", year = "2018", abstract = "Ergosterol (Erg) is the major sterol component in the plasma membranes of fungi. The main goal of this work was to develop rapid, reliable, and cost-effective method toward Erg detection based on application of unmodified boron-doped diamond electrode. Several organic solvents were tested (acetonitrile, dimethyformamide, dimethylsulfoxide, dichloromethane), and it was found that best analytical characteristics were achieved using acetonitrile containing 0.1 M tetrabutylammonium hexa-fluorophosphate as supporting electrolyte. Oval shaped and well-defined oxidation peak was recorded at potential of around 1.17 V versus Ag/Ag+ non-aqueous reference electrode. Under optimized experimental conditions, linear working range from 2 to 200 mu M was estimated, with limit of detection of 0.7 mu M, using square wave voltammetry. Negligible effect of possible interfering compound was observed. Proposed methodology was successfully applied for estimation of Erg content in mushrooms extract with satisfactory results in comparison with spectrophotometric procedure. Obtained results clearly show that developed analytical methodology can be adequate replacement for the, up to date, used methods and open-novel approach for ergosterol detection.", journal = "Food Analytical Methods", title = "Analytical Approach for Detection of Ergosterol in Mushrooms Based on Modification Free Electrochemical Sensor in Organic Solvents", volume = "11", number = "9", pages = "2590-2596", doi = "10.1007/s12161-018-1249-3" }
Vukojević, V., Đurđić, S., Švorc, L., Ćirković Veličković, T., Mutić, J. J.,& Stanković, D. M.. (2018). Analytical Approach for Detection of Ergosterol in Mushrooms Based on Modification Free Electrochemical Sensor in Organic Solvents. in Food Analytical Methods, 11(9), 2590-2596. https://doi.org/10.1007/s12161-018-1249-3
Vukojević V, Đurđić S, Švorc L, Ćirković Veličković T, Mutić JJ, Stanković DM. Analytical Approach for Detection of Ergosterol in Mushrooms Based on Modification Free Electrochemical Sensor in Organic Solvents. in Food Analytical Methods. 2018;11(9):2590-2596. doi:10.1007/s12161-018-1249-3 .
Vukojević, Vesna, Đurđić, Slađana, Švorc, Lubomir, Ćirković Veličković, Tanja, Mutić, Jelena J., Stanković, Dalibor M., "Analytical Approach for Detection of Ergosterol in Mushrooms Based on Modification Free Electrochemical Sensor in Organic Solvents" in Food Analytical Methods, 11, no. 9 (2018):2590-2596, https://doi.org/10.1007/s12161-018-1249-3 . .