Proteomic characterization of hippocampus of chronically socially isolated rats treated with fluoxetine: Depression-like behaviour and fluoxetine mechanism of action
Само за регистроване кориснике
2018
Чланак у часопису (Објављена верзија)
,
© 2018 Elsevier Ltd
Метаподаци
Приказ свих података о документуАпстракт
Due to the severity of depressive symptoms, there remains a necessity in defining the underlying mechanisms of depression and the precise actions of antidepressants in alleviating these symptoms. Proteomics is a powerful and promising tool for discovering novel pathways of cellular responses to disease and treatment. As chronic social isolation (CSIS) is a valuable animal model for studying depression, we performed a comparative subproteomic study of rat hippocampus to explore the effect of six weeks of CSIS and the therapeutic effect of chronic fluoxetine (Flx) treatment (last three weeks of CSIS; 15 mg/kg/day). Behaviorally, Flx treatment normalized the decreased sucrose preference and increased marble burying results resulting from CSIS, indicative of a FLX-induced attenuation of both anhedonia and anxiety. An analysis of cytosolic and nonsynaptic mitochondrial subproteome patterns revealed that CSIS resulted in down-regulation of proteins involved in mitochondrial transport and ene...rgy processes, primarily tricarboxylic acid (TCA) cycle and oxidative phosphorylation. Chronic Flx treatment resulted in an up-regulation of CSIS-altered proteins and additional expression of other transporter and energy-involved proteins. Immunohistochemical analysis revealed hippocampal subregion-specific effects of CSIS and/or Flx treatment on selective protein expressions. (C) 2018 Elsevier Ltd. All rights reserved.
Кључне речи:
Fluoxetine / Depressive disorder / Hippocampus / Quantitative proteomics / Protein interactomicsИзвор:
Neuropharmacology, 2018, 135, 268-283Финансирање / пројекти:
DOI: 10.1016/j.neuropharm.2018.03.034
ISSN: 0028-3908; 1873-7064
PubMed: 29596899
WoS: 000436915600024
Scopus: 2-s2.0-85044504379
URI
https://linkinghub.elsevier.com/retrieve/pii/S0028390818301461https://vinar.vin.bg.ac.rs/handle/123456789/7913
Колекције
Институција/група
VinčaTY - JOUR AU - Perić, Ivana AU - Costina, Victor AU - Stanisavljević, Andrijana AU - Findeisen, Peter AU - Filipović, Dragana PY - 2018 UR - https://linkinghub.elsevier.com/retrieve/pii/S0028390818301461 UR - https://vinar.vin.bg.ac.rs/handle/123456789/7913 AB - Due to the severity of depressive symptoms, there remains a necessity in defining the underlying mechanisms of depression and the precise actions of antidepressants in alleviating these symptoms. Proteomics is a powerful and promising tool for discovering novel pathways of cellular responses to disease and treatment. As chronic social isolation (CSIS) is a valuable animal model for studying depression, we performed a comparative subproteomic study of rat hippocampus to explore the effect of six weeks of CSIS and the therapeutic effect of chronic fluoxetine (Flx) treatment (last three weeks of CSIS; 15 mg/kg/day). Behaviorally, Flx treatment normalized the decreased sucrose preference and increased marble burying results resulting from CSIS, indicative of a FLX-induced attenuation of both anhedonia and anxiety. An analysis of cytosolic and nonsynaptic mitochondrial subproteome patterns revealed that CSIS resulted in down-regulation of proteins involved in mitochondrial transport and energy processes, primarily tricarboxylic acid (TCA) cycle and oxidative phosphorylation. Chronic Flx treatment resulted in an up-regulation of CSIS-altered proteins and additional expression of other transporter and energy-involved proteins. Immunohistochemical analysis revealed hippocampal subregion-specific effects of CSIS and/or Flx treatment on selective protein expressions. (C) 2018 Elsevier Ltd. All rights reserved. T2 - Neuropharmacology T1 - Proteomic characterization of hippocampus of chronically socially isolated rats treated with fluoxetine: Depression-like behaviour and fluoxetine mechanism of action VL - 135 SP - 268 EP - 283 DO - 10.1016/j.neuropharm.2018.03.034 ER -
@article{ author = "Perić, Ivana and Costina, Victor and Stanisavljević, Andrijana and Findeisen, Peter and Filipović, Dragana", year = "2018", abstract = "Due to the severity of depressive symptoms, there remains a necessity in defining the underlying mechanisms of depression and the precise actions of antidepressants in alleviating these symptoms. Proteomics is a powerful and promising tool for discovering novel pathways of cellular responses to disease and treatment. As chronic social isolation (CSIS) is a valuable animal model for studying depression, we performed a comparative subproteomic study of rat hippocampus to explore the effect of six weeks of CSIS and the therapeutic effect of chronic fluoxetine (Flx) treatment (last three weeks of CSIS; 15 mg/kg/day). Behaviorally, Flx treatment normalized the decreased sucrose preference and increased marble burying results resulting from CSIS, indicative of a FLX-induced attenuation of both anhedonia and anxiety. An analysis of cytosolic and nonsynaptic mitochondrial subproteome patterns revealed that CSIS resulted in down-regulation of proteins involved in mitochondrial transport and energy processes, primarily tricarboxylic acid (TCA) cycle and oxidative phosphorylation. Chronic Flx treatment resulted in an up-regulation of CSIS-altered proteins and additional expression of other transporter and energy-involved proteins. Immunohistochemical analysis revealed hippocampal subregion-specific effects of CSIS and/or Flx treatment on selective protein expressions. (C) 2018 Elsevier Ltd. All rights reserved.", journal = "Neuropharmacology", title = "Proteomic characterization of hippocampus of chronically socially isolated rats treated with fluoxetine: Depression-like behaviour and fluoxetine mechanism of action", volume = "135", pages = "268-283", doi = "10.1016/j.neuropharm.2018.03.034" }
Perić, I., Costina, V., Stanisavljević, A., Findeisen, P.,& Filipović, D.. (2018). Proteomic characterization of hippocampus of chronically socially isolated rats treated with fluoxetine: Depression-like behaviour and fluoxetine mechanism of action. in Neuropharmacology, 135, 268-283. https://doi.org/10.1016/j.neuropharm.2018.03.034
Perić I, Costina V, Stanisavljević A, Findeisen P, Filipović D. Proteomic characterization of hippocampus of chronically socially isolated rats treated with fluoxetine: Depression-like behaviour and fluoxetine mechanism of action. in Neuropharmacology. 2018;135:268-283. doi:10.1016/j.neuropharm.2018.03.034 .
Perić, Ivana, Costina, Victor, Stanisavljević, Andrijana, Findeisen, Peter, Filipović, Dragana, "Proteomic characterization of hippocampus of chronically socially isolated rats treated with fluoxetine: Depression-like behaviour and fluoxetine mechanism of action" in Neuropharmacology, 135 (2018):268-283, https://doi.org/10.1016/j.neuropharm.2018.03.034 . .