DUV fluorescence bioimaging study of the interaction of partially reduced graphene oxide and liver cancer cells
Pajović, Jelena D.
Božanić, Dušan K.
Jović, Nataša G.
Pavlović, Vera P.
Pavlović, Vladimir B.
Lenhardt Acković, Lea
Zeković, Ivana Lj.
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The interaction of partially reduced graphene oxide (prGO) and Huh7.5.1 liver cancer cells was investigated by means of DUV fluorescence bioimaging. The prGO sample was obtained by the reduction (to a certain extent) of the initially prepared graphene oxide (GO) nanosheets with hydrazine. The fluorescence of the GO nanosheets increases with time of the reduction due to a change in ratio of the sp2 and sp3 carbon sites and the prGO sample was extracted from the dispersion after 6 min, when the intensity of the fluorescence reached its maximum. The reduction process was left to proceed further to saturation until highly reduced graphene oxide (denoted here as rGO) was obtained. GO, prGO and rGO samples were investigated by structural (scanning electron microscopy (SEM), scanning transmission electron microscopy coupled with energy dispersive spectrometry (STEM-EDS)) and spectroscopic (UV-vis, photoluminescence (PL), Raman) methods. After that, Huh7.5.1 cells were incubated with GO, prGO ...and rGO nanosheets and used in bioimaging studies, which were performed on DISCO beamline of synchrotron SOLEIL. It was found that the prGO significantly enhanced the fluorescence of the cells and increased the intensity of the signal by ~2.5 times. Time-lapse fluorescence microscopy experiments showed that fluorescence dynamics strongly depends on the type of nanosheets used. The obtained prGO nanostructure can be easily conjugated with aromatic ring containing drugs, which opens a possibility for its applications in fluorescence microscopy monitored drug delivery. © 2018 IOP Publishing Ltd.
Ključne reči:bioimaging / graphene oxide / fluorescence / cancer / cells
Izvor:2D Materials, 2018, 5, 4, 045019-
- Uticaj veličine, oblika i strukture nanočestica na njihova svojstva i svojstva nanokompozita (RS-172056)
- Materijali redukovane dimenzionalnosti za efikasnu apsorpciju svetlosti i konverziju energije (RS-45020)
- Proučavanje uticaja tretiranja na dielektrične, optičke, magnetne i osobine površina kristalnih i polimernih sistema (RS-171029)
- NSF CREST (HRD-0833184)
- NASA (NNX09AV07A)
ISSN: 2053-1583 (print)