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dc.creatorBožić, Bojana
dc.creatorKorać, Jelena
dc.creatorStanković, Dalibor M.
dc.creatorStanić, Marina
dc.creatorRomanović, Mima Č.
dc.creatorBogdanović-Pristov, Jelena
dc.creatorSpasić, Snežana D.
dc.creatorPopović-Bijelić, Ana D.
dc.creatorSpasojević, Ivan
dc.creatorBajčetić, Milica
dc.date.accessioned2018-10-30T08:04:51Z
dc.date.available2018-10-30T08:04:51Z
dc.date.issued2018
dc.identifier.issn0891-5849 (print)
dc.identifier.issn1873-4596 (electrical)
dc.identifier.urihttps://linkinghub.elsevier.com/retrieve/pii/S0891584918311213
dc.identifier.urihttp://vinar.vin.bg.ac.rs/handle/123456789/7896
dc.description.abstractAn increase in the copper pool in body fluids has been related to a number of pathological conditions, including infections. Copper ions may affect antibiotics via the formation of coordination bonds and/or redox reactions. Herein, we analyzed the interactions of Cu2+ with eight β-lactam antibiotics using UV–Vis spectrophotometry, EPR spectroscopy, and electrochemical methods. Penicillin G did not show any detectable interactions with Cu2+. Ampicillin, amoxicillin and cephalexin formed stable colored complexes with octahedral coordination environment of Cu2+ with tetragonal distortion, and primary amine group as the site of coordinate bond formation. These β-lactams increased the solubility of Cu2+ in the phosphate buffer. Ceftazidime and Cu2+ formed a complex with a similar geometry and gave rise to an organic radical. Ceftriaxone-Cu2+ complex appears to exhibit different geometry. All complexes showed 1:1 stoichiometry. Cefaclor reduced Cu2+ to Cu1+ that further reacted with molecular oxygen to produce hydrogen peroxide. Finally, meropenem underwent degradation in the presence of copper. The analysis of activity against Escherichia coli and Staphylococcus aureus showed that the effects of meropenem, amoxicillin, ampicillin, and ceftriaxone were significantly hindered in the presence of copper ions. The interactions with copper ions should be taken into account regarding the problem of antibiotic resistance and in the selection of the most efficient antimicrobial therapy for patients with altered copper homeostasis. © 2018 Elsevier Inc.
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/621375/EU//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173014/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173017/RS//
dc.rightsrestrictedAccess
dc.sourceFree Radical Biology and Medicine
dc.subjectAntibioticen
dc.subjectComplexen
dc.subjectCopperen
dc.subjectEPR spectroscopyen
dc.subjectFree radicalsen
dc.titleCoordination and redox interactions of β-lactam antibiotics with Cu2+ in physiological settings and the impact on antibacterial activityen
dc.typearticleen
dc.rights.licenseARR
dcterms.abstractРомановић, Мима Ч.; Спасић, Снежана Д.; Поповић-Бијелић, Aна Д.; Спасојевић, Иван; Бајчетић, Милица; Богдановић-Пристов, Јелена; Божић, Бојана; Кораћ, Јелена; Станковић, Далибор М.; Станић, Марина;
dc.rights.holder© 2018 Elsevier Inc
dc.citation.volume129
dc.citation.spage279
dc.citation.epage285
dc.identifier.wos000450298400026
dc.identifier.doi10.1016/j.freeradbiomed.2018.09.038
dc.identifier.pmid30267756
dc.type.versionpublishedVersion
dc.identifier.scopus2-s2.0-85054184646


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