Manuka honey attenuates oxidative damage induced by H2O2 in human whole blood in vitro
Само за регистроване кориснике
2018
Аутори
Živković, LadaBajić, Vladan P.
Dekanski, Dragana
Čabarkapa-Pirković, Andrea
Giampieri, Francesca
Gasparrini, Massimiliano
Mazzoni, Luca
Spremo-Potparević, Biljana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Manuka honey has been widely researched regarding its biological properties, in particular its antimicrobial and antioxidant capacities. We tested the genotoxic and genoprotective properties of Manuka honey, ranging from 25–1000 μg/mL, by performing an in vitro comet assay after exposure to human whole blood. No genotoxic effect on whole blood cells was observed within the tested concentration range (p = 0.154). Then, the antigenotoxic potency of Manuka honey against oxidative DNA damage to whole blood cells was assessed. Prior to Manuka honey treatment a modest decrease of H2O2-induced DNA damage was detected in cells, with no statistical significance (p = 0.087). Post-treatment, Manuka honey displayed a stronger potential to attenuate damaged cells at all tested concentrations, with a statistical significant difference (p < 0.001), where concentrations of 25 and 100 μg/mL were most efficient. Manuka honey exhibited a marked potential to protect DNA of whole blood cells from oxidative... damage induced by hydrogen peroxide in vitro.
Извор:
Food and Chemical Toxicology, 2018, 119, 61-65Финансирање / пројекти:
- Аберације ћелијског циклуса и утицај оксидативног стреса на неуродегенеративне процесе и малигну трансформацију ћелије (RS-MESTD-Basic Research (BR or ON)-173034)
- hCOMET COST action (No. 15132)
DOI: 10.1016/j.fct.2018.05.034
ISSN: 0278-6915
WoS: 000443664200009
Scopus: 2-s2.0-85048158869
URI
https://linkinghub.elsevier.com/retrieve/pii/S027869151830334Xhttps://vinar.vin.bg.ac.rs/handle/123456789/7819
Колекције
Институција/група
VinčaTY - JOUR AU - Živković, Lada AU - Bajić, Vladan P. AU - Dekanski, Dragana AU - Čabarkapa-Pirković, Andrea AU - Giampieri, Francesca AU - Gasparrini, Massimiliano AU - Mazzoni, Luca AU - Spremo-Potparević, Biljana PY - 2018 UR - https://linkinghub.elsevier.com/retrieve/pii/S027869151830334X UR - https://vinar.vin.bg.ac.rs/handle/123456789/7819 AB - Manuka honey has been widely researched regarding its biological properties, in particular its antimicrobial and antioxidant capacities. We tested the genotoxic and genoprotective properties of Manuka honey, ranging from 25–1000 μg/mL, by performing an in vitro comet assay after exposure to human whole blood. No genotoxic effect on whole blood cells was observed within the tested concentration range (p = 0.154). Then, the antigenotoxic potency of Manuka honey against oxidative DNA damage to whole blood cells was assessed. Prior to Manuka honey treatment a modest decrease of H2O2-induced DNA damage was detected in cells, with no statistical significance (p = 0.087). Post-treatment, Manuka honey displayed a stronger potential to attenuate damaged cells at all tested concentrations, with a statistical significant difference (p < 0.001), where concentrations of 25 and 100 μg/mL were most efficient. Manuka honey exhibited a marked potential to protect DNA of whole blood cells from oxidative damage induced by hydrogen peroxide in vitro. T2 - Food and Chemical Toxicology T1 - Manuka honey attenuates oxidative damage induced by H2O2 in human whole blood in vitro VL - 119 SP - 61 EP - 65 DO - 10.1016/j.fct.2018.05.034 ER -
@article{ author = "Živković, Lada and Bajić, Vladan P. and Dekanski, Dragana and Čabarkapa-Pirković, Andrea and Giampieri, Francesca and Gasparrini, Massimiliano and Mazzoni, Luca and Spremo-Potparević, Biljana", year = "2018", abstract = "Manuka honey has been widely researched regarding its biological properties, in particular its antimicrobial and antioxidant capacities. We tested the genotoxic and genoprotective properties of Manuka honey, ranging from 25–1000 μg/mL, by performing an in vitro comet assay after exposure to human whole blood. No genotoxic effect on whole blood cells was observed within the tested concentration range (p = 0.154). Then, the antigenotoxic potency of Manuka honey against oxidative DNA damage to whole blood cells was assessed. Prior to Manuka honey treatment a modest decrease of H2O2-induced DNA damage was detected in cells, with no statistical significance (p = 0.087). Post-treatment, Manuka honey displayed a stronger potential to attenuate damaged cells at all tested concentrations, with a statistical significant difference (p < 0.001), where concentrations of 25 and 100 μg/mL were most efficient. Manuka honey exhibited a marked potential to protect DNA of whole blood cells from oxidative damage induced by hydrogen peroxide in vitro.", journal = "Food and Chemical Toxicology", title = "Manuka honey attenuates oxidative damage induced by H2O2 in human whole blood in vitro", volume = "119", pages = "61-65", doi = "10.1016/j.fct.2018.05.034" }
Živković, L., Bajić, V. P., Dekanski, D., Čabarkapa-Pirković, A., Giampieri, F., Gasparrini, M., Mazzoni, L.,& Spremo-Potparević, B.. (2018). Manuka honey attenuates oxidative damage induced by H2O2 in human whole blood in vitro. in Food and Chemical Toxicology, 119, 61-65. https://doi.org/10.1016/j.fct.2018.05.034
Živković L, Bajić VP, Dekanski D, Čabarkapa-Pirković A, Giampieri F, Gasparrini M, Mazzoni L, Spremo-Potparević B. Manuka honey attenuates oxidative damage induced by H2O2 in human whole blood in vitro. in Food and Chemical Toxicology. 2018;119:61-65. doi:10.1016/j.fct.2018.05.034 .
Živković, Lada, Bajić, Vladan P., Dekanski, Dragana, Čabarkapa-Pirković, Andrea, Giampieri, Francesca, Gasparrini, Massimiliano, Mazzoni, Luca, Spremo-Potparević, Biljana, "Manuka honey attenuates oxidative damage induced by H2O2 in human whole blood in vitro" in Food and Chemical Toxicology, 119 (2018):61-65, https://doi.org/10.1016/j.fct.2018.05.034 . .