RuO2/graphene nanoribbon composite supported on screen printed electrode with enhanced electrocatalytic performances toward ethanol and NADH biosensing
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2018
Authors
Vukojević, VesnaĐurđić, Slađana
Ognjanović, Miloš
Antić, Bratislav
Kalcher, Kurt
Mutić, Jelena J.
Stanković, Dalibor M.
Article (Published version)
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© 2018 Elsevier B.V.
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In this work, we aimed to propose a newly synthesized composite material with enhanced electrocatalytic properties as a novel screen-printed sensor for the quantification of NADH. Additionally, the surface was modified with alcohol dehydrogenase for the preparation of an amperometric biosensor for analysis of ethanol. Synthesized material was characterized using several microstructural (FE-SEM, HR-TEM, XRD) and electrochemical (CV, EIS) techniques. The electrochemical response of the tested analytes was investigated as a function of important parameters. Under optimal conditions, the working linear range and limit of detection for ethanol sensing was 1–1800 µM and 0.19 µM, respectively. For NADH, the linear range was from 1 to 1300 µM with limit of detection of 0.52 µM. Moreover, effects of some possible interfering compounds were investigated and the developed procedure was applied to commercial alcoholic beverages. The results obtained showed satisfactory precision and accuracy of th...e developed method and confirm the proposed approach could be a possible replacement for the currently used techniques for ethanol and NADH quantification.
Keywords:
alcohol dehydrogenase / ethanol biosensor / graphene nanoribbons / screen printed electrodeSource:
Biosensors and Bioelectronics, 2018, 117, 392-397Funding / projects:
- Application of advanced oxidation processes and nanostructured oxide materials for the removal of pollutants from the environment, development and optimisation of instrumental techniques for efficiency monitoring (RS-MESTD-Basic Research (BR or ON)-172030)
DOI: 10.1016/j.bios.2018.06.038
ISSN: 0956-5663
WoS: 000442191900047
Scopus: 2-s2.0-85049075205
URI
https://linkinghub.elsevier.com/retrieve/pii/S0956566318304688https://vinar.vin.bg.ac.rs/handle/123456789/7766
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VinčaTY - JOUR AU - Vukojević, Vesna AU - Đurđić, Slađana AU - Ognjanović, Miloš AU - Antić, Bratislav AU - Kalcher, Kurt AU - Mutić, Jelena J. AU - Stanković, Dalibor M. PY - 2018 UR - https://linkinghub.elsevier.com/retrieve/pii/S0956566318304688 UR - https://vinar.vin.bg.ac.rs/handle/123456789/7766 AB - In this work, we aimed to propose a newly synthesized composite material with enhanced electrocatalytic properties as a novel screen-printed sensor for the quantification of NADH. Additionally, the surface was modified with alcohol dehydrogenase for the preparation of an amperometric biosensor for analysis of ethanol. Synthesized material was characterized using several microstructural (FE-SEM, HR-TEM, XRD) and electrochemical (CV, EIS) techniques. The electrochemical response of the tested analytes was investigated as a function of important parameters. Under optimal conditions, the working linear range and limit of detection for ethanol sensing was 1–1800 µM and 0.19 µM, respectively. For NADH, the linear range was from 1 to 1300 µM with limit of detection of 0.52 µM. Moreover, effects of some possible interfering compounds were investigated and the developed procedure was applied to commercial alcoholic beverages. The results obtained showed satisfactory precision and accuracy of the developed method and confirm the proposed approach could be a possible replacement for the currently used techniques for ethanol and NADH quantification. T2 - Biosensors and Bioelectronics T1 - RuO2/graphene nanoribbon composite supported on screen printed electrode with enhanced electrocatalytic performances toward ethanol and NADH biosensing VL - 117 SP - 392 EP - 397 DO - 10.1016/j.bios.2018.06.038 ER -
@article{ author = "Vukojević, Vesna and Đurđić, Slađana and Ognjanović, Miloš and Antić, Bratislav and Kalcher, Kurt and Mutić, Jelena J. and Stanković, Dalibor M.", year = "2018", abstract = "In this work, we aimed to propose a newly synthesized composite material with enhanced electrocatalytic properties as a novel screen-printed sensor for the quantification of NADH. Additionally, the surface was modified with alcohol dehydrogenase for the preparation of an amperometric biosensor for analysis of ethanol. Synthesized material was characterized using several microstructural (FE-SEM, HR-TEM, XRD) and electrochemical (CV, EIS) techniques. The electrochemical response of the tested analytes was investigated as a function of important parameters. Under optimal conditions, the working linear range and limit of detection for ethanol sensing was 1–1800 µM and 0.19 µM, respectively. For NADH, the linear range was from 1 to 1300 µM with limit of detection of 0.52 µM. Moreover, effects of some possible interfering compounds were investigated and the developed procedure was applied to commercial alcoholic beverages. The results obtained showed satisfactory precision and accuracy of the developed method and confirm the proposed approach could be a possible replacement for the currently used techniques for ethanol and NADH quantification.", journal = "Biosensors and Bioelectronics", title = "RuO2/graphene nanoribbon composite supported on screen printed electrode with enhanced electrocatalytic performances toward ethanol and NADH biosensing", volume = "117", pages = "392-397", doi = "10.1016/j.bios.2018.06.038" }
Vukojević, V., Đurđić, S., Ognjanović, M., Antić, B., Kalcher, K., Mutić, J. J.,& Stanković, D. M.. (2018). RuO2/graphene nanoribbon composite supported on screen printed electrode with enhanced electrocatalytic performances toward ethanol and NADH biosensing. in Biosensors and Bioelectronics, 117, 392-397. https://doi.org/10.1016/j.bios.2018.06.038
Vukojević V, Đurđić S, Ognjanović M, Antić B, Kalcher K, Mutić JJ, Stanković DM. RuO2/graphene nanoribbon composite supported on screen printed electrode with enhanced electrocatalytic performances toward ethanol and NADH biosensing. in Biosensors and Bioelectronics. 2018;117:392-397. doi:10.1016/j.bios.2018.06.038 .
Vukojević, Vesna, Đurđić, Slađana, Ognjanović, Miloš, Antić, Bratislav, Kalcher, Kurt, Mutić, Jelena J., Stanković, Dalibor M., "RuO2/graphene nanoribbon composite supported on screen printed electrode with enhanced electrocatalytic performances toward ethanol and NADH biosensing" in Biosensors and Bioelectronics, 117 (2018):392-397, https://doi.org/10.1016/j.bios.2018.06.038 . .