Confocal micrographs: automated segmentation and quantitative shape analysis of neuronal cells treated with ostreolysin A/pleurotolysin B pore-forming complex
Само за регистроване кориснике
2018
Аутори
Kopanja, LazarKovačević, Zorana
Tadić, Marin
Žužek, Monika Cecilija
Vrecl, Milka
Frangež, Robert
Чланак у часопису (Објављена верзија)
,
© 2018, Springer-Verlag GmbH Germany, part of Springer Nature
Метаподаци
Приказ свих података о документуАпстракт
Detailed shape analysis of cells is important to better understand the physiological mechanisms of toxins and determine their effects on cell morphology. This study aimed to develop a procedure for accurate morphological analysis of cell shape and use it as a tool to estimate toxin activity. With the aim of optimizing the method of cell morphology analysis, we determined the influence of ostreolysin A and pleurotolysin B complex (OlyA/PlyB) on the morphology of murine neuronal NG108-15 cells. A computational method was introduced and successfully applied to quantify morphological attributes of the NG108-15 cell line before and after 30 and 60 min exposure to OlyA/PlyB using confocal microscopy. The modified circularity measure (Formula presented.) for shape analysis was applied, which defines the degree to which the shape of the neuron differs from a perfect circle. It enables better detection of small changes in the shape of cells, making the outcome easily detectable numerically. Add...itionally, we analyzed the influence of OlyA/PlyB on the cell area, allowing us to detect the cells with blebs. This is important because the formation of plasma membrane protrusions such as blebs often reflects cell injury that leads to necrotic cell death. In summary, we offer a novel analytical method of neuronal cell shape analysis and its correlation with the toxic effects of the pore-forming OlyA/PlyB toxin in situ.
Кључне речи:
image analyses / confocal microscopy / cell morphology / cell shape / ostreolysin A/pleurotolysin BИзвор:
Histochemistry and Cell Biology, 2018, 150, 1, 93-102Финансирање / пројекти:
- Serbian Ministry of Education, Science and Technological Development & Slovenian Research Agency (P4-0053)
DOI: 10.1007/s00418-018-1670-0
ISSN: 0948-6143; 1432-119X
PubMed: 29687242
WoS: 000436854000009
Scopus: 2-s2.0-85045856720
URI
http://link.springer.com/10.1007/s00418-018-1670-0https://vinar.vin.bg.ac.rs/handle/123456789/7762
Колекције
Институција/група
VinčaTY - JOUR AU - Kopanja, Lazar AU - Kovačević, Zorana AU - Tadić, Marin AU - Žužek, Monika Cecilija AU - Vrecl, Milka AU - Frangež, Robert PY - 2018 UR - http://link.springer.com/10.1007/s00418-018-1670-0 UR - https://vinar.vin.bg.ac.rs/handle/123456789/7762 AB - Detailed shape analysis of cells is important to better understand the physiological mechanisms of toxins and determine their effects on cell morphology. This study aimed to develop a procedure for accurate morphological analysis of cell shape and use it as a tool to estimate toxin activity. With the aim of optimizing the method of cell morphology analysis, we determined the influence of ostreolysin A and pleurotolysin B complex (OlyA/PlyB) on the morphology of murine neuronal NG108-15 cells. A computational method was introduced and successfully applied to quantify morphological attributes of the NG108-15 cell line before and after 30 and 60 min exposure to OlyA/PlyB using confocal microscopy. The modified circularity measure (Formula presented.) for shape analysis was applied, which defines the degree to which the shape of the neuron differs from a perfect circle. It enables better detection of small changes in the shape of cells, making the outcome easily detectable numerically. Additionally, we analyzed the influence of OlyA/PlyB on the cell area, allowing us to detect the cells with blebs. This is important because the formation of plasma membrane protrusions such as blebs often reflects cell injury that leads to necrotic cell death. In summary, we offer a novel analytical method of neuronal cell shape analysis and its correlation with the toxic effects of the pore-forming OlyA/PlyB toxin in situ. T2 - Histochemistry and Cell Biology T1 - Confocal micrographs: automated segmentation and quantitative shape analysis of neuronal cells treated with ostreolysin A/pleurotolysin B pore-forming complex VL - 150 IS - 1 SP - 93 EP - 102 DO - 10.1007/s00418-018-1670-0 ER -
@article{ author = "Kopanja, Lazar and Kovačević, Zorana and Tadić, Marin and Žužek, Monika Cecilija and Vrecl, Milka and Frangež, Robert", year = "2018", abstract = "Detailed shape analysis of cells is important to better understand the physiological mechanisms of toxins and determine their effects on cell morphology. This study aimed to develop a procedure for accurate morphological analysis of cell shape and use it as a tool to estimate toxin activity. With the aim of optimizing the method of cell morphology analysis, we determined the influence of ostreolysin A and pleurotolysin B complex (OlyA/PlyB) on the morphology of murine neuronal NG108-15 cells. A computational method was introduced and successfully applied to quantify morphological attributes of the NG108-15 cell line before and after 30 and 60 min exposure to OlyA/PlyB using confocal microscopy. The modified circularity measure (Formula presented.) for shape analysis was applied, which defines the degree to which the shape of the neuron differs from a perfect circle. It enables better detection of small changes in the shape of cells, making the outcome easily detectable numerically. Additionally, we analyzed the influence of OlyA/PlyB on the cell area, allowing us to detect the cells with blebs. This is important because the formation of plasma membrane protrusions such as blebs often reflects cell injury that leads to necrotic cell death. In summary, we offer a novel analytical method of neuronal cell shape analysis and its correlation with the toxic effects of the pore-forming OlyA/PlyB toxin in situ.", journal = "Histochemistry and Cell Biology", title = "Confocal micrographs: automated segmentation and quantitative shape analysis of neuronal cells treated with ostreolysin A/pleurotolysin B pore-forming complex", volume = "150", number = "1", pages = "93-102", doi = "10.1007/s00418-018-1670-0" }
Kopanja, L., Kovačević, Z., Tadić, M., Žužek, M. C., Vrecl, M.,& Frangež, R.. (2018). Confocal micrographs: automated segmentation and quantitative shape analysis of neuronal cells treated with ostreolysin A/pleurotolysin B pore-forming complex. in Histochemistry and Cell Biology, 150(1), 93-102. https://doi.org/10.1007/s00418-018-1670-0
Kopanja L, Kovačević Z, Tadić M, Žužek MC, Vrecl M, Frangež R. Confocal micrographs: automated segmentation and quantitative shape analysis of neuronal cells treated with ostreolysin A/pleurotolysin B pore-forming complex. in Histochemistry and Cell Biology. 2018;150(1):93-102. doi:10.1007/s00418-018-1670-0 .
Kopanja, Lazar, Kovačević, Zorana, Tadić, Marin, Žužek, Monika Cecilija, Vrecl, Milka, Frangež, Robert, "Confocal micrographs: automated segmentation and quantitative shape analysis of neuronal cells treated with ostreolysin A/pleurotolysin B pore-forming complex" in Histochemistry and Cell Biology, 150, no. 1 (2018):93-102, https://doi.org/10.1007/s00418-018-1670-0 . .