Hydrothermally processed 1D hydroxyapatite: Mechanism of formation and biocompatibility studies
Аутори
Stojanović, Zoran S.Ignjatović, Nenad L.
Wu, Victoria
Žunič, Vojka
Veselinović, Ljiljana M.
Škapin, Srečo Davor
Miljković, Miroslav
Uskoković, Vuk
Uskoković, Dragan
Чланак у часопису (Рецензирана верзија)
Метаподаци
Приказ свих података о документуАпстракт
Recent developments in bone tissue engineering have led to an increased interest in one-dimensional (1D) hydroxyapatite (HA) nano- and micro-structures such as wires, ribbons and tubes. They have been proposed for use as cell substrates, reinforcing phases in composites and carriers for biologically active substances. Here we demonstrate the synthesis of 1D HA structures using an optimized, urea-assisted, high-yield hydrothermal batch process. The one-pot process, yielding HA structures composed of bundles of ribbons and wires, was typified by the simultaneous occurrence of a multitude of intermediate reactions, failing to meet the uniformity criteria over particle morphology and size. To overcome these issues, the preparation procedure was divided to two stages: dicalcium phosphate platelets synthesized in the first step were used as a precursor for the synthesis of 1D HA in the second stage. Despite the elongated particle morphologies, both the precursor and the final product exhibit...ed excellent biocompatibility and caused no reduction of viability when tested against osteoblastic MC3T3-E1 cells in 2D culture up to the concentration of 2.6 mg/cm2. X-ray powder diffraction combined with a range of electron microscopies and laser diffraction analyses was used to elucidate the formation mechanism and the microstructure of the final particles. The two-step synthesis involved a more direct transformation of DCP to 1D HA with the average diameter of 37 nm and the aspect ratio exceeding 100:1. The comparison of crystalline domain sizes along different crystallographic directions showed no signs of significant anisotropy, while indicating that individual nanowires are ordered in bundles in the b crystallographic direction of the P63/m space group of HA. Intermediate processes, e.g., dehydration of dicalcium phosphate, are critical for the formation of 1D HA alongside other key aspects of this phase transformation, it must be investigated in more detail in the continuous design of smart HA micro- and nano-structures with advanced therapeutic potentials.
Кључне речи:
hydrothermal processing / nanowires / hydroxyapatite / biomedical materials / particle size distributionИзвор:
Materials Science and Engineering: C, 2016, 68, 746-757Финансирање / пројекти:
- Молекуларно дизајнирање наночестица контролисаних морфолошких и физичко-хемијских карактеристика и функционалних материјала на њиховој основи (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-45004)
- National Institute of Health grant R00-DE021416
Напомена:
- This is the peer-reviewed version of the articleStojanović, Z.S., Ignjatović, N., Wu, V., Žunič, V., Veselinović, L., Škapin, S., Miljković, M., Uskoković, V., Uskoković, D., 2016. Hydrothermally processed 1D hydroxyapatite: Mechanism of formation and biocompatibility studies. Materials Science and Engineering: C 68, 746–757. https://doi.org/10.1016/j.msec.2016.06.047
- Published version: https://vinar.vin.bg.ac.rs/handle/123456789/7576
Повезане информације:
- Друга верзија
http://dx.doi.org/10.1016/j.msec.2016.06.047 - Друга верзија
https://vinar.vin.bg.ac.rs/handle/123456789/7576
DOI: 10.1016/j.msec.2016.06.047
ISSN: 0928-4931
WoS: 000382600000088
Scopus: 2-s2.0-84975886009
Колекције
Институција/група
VinčaTY - JOUR AU - Stojanović, Zoran S. AU - Ignjatović, Nenad L. AU - Wu, Victoria AU - Žunič, Vojka AU - Veselinović, Ljiljana M. AU - Škapin, Srečo Davor AU - Miljković, Miroslav AU - Uskoković, Vuk AU - Uskoković, Dragan PY - 2016 UR - https://vinar.vin.bg.ac.rs/handle/123456789/7575 AB - Recent developments in bone tissue engineering have led to an increased interest in one-dimensional (1D) hydroxyapatite (HA) nano- and micro-structures such as wires, ribbons and tubes. They have been proposed for use as cell substrates, reinforcing phases in composites and carriers for biologically active substances. Here we demonstrate the synthesis of 1D HA structures using an optimized, urea-assisted, high-yield hydrothermal batch process. The one-pot process, yielding HA structures composed of bundles of ribbons and wires, was typified by the simultaneous occurrence of a multitude of intermediate reactions, failing to meet the uniformity criteria over particle morphology and size. To overcome these issues, the preparation procedure was divided to two stages: dicalcium phosphate platelets synthesized in the first step were used as a precursor for the synthesis of 1D HA in the second stage. Despite the elongated particle morphologies, both the precursor and the final product exhibited excellent biocompatibility and caused no reduction of viability when tested against osteoblastic MC3T3-E1 cells in 2D culture up to the concentration of 2.6 mg/cm2. X-ray powder diffraction combined with a range of electron microscopies and laser diffraction analyses was used to elucidate the formation mechanism and the microstructure of the final particles. The two-step synthesis involved a more direct transformation of DCP to 1D HA with the average diameter of 37 nm and the aspect ratio exceeding 100:1. The comparison of crystalline domain sizes along different crystallographic directions showed no signs of significant anisotropy, while indicating that individual nanowires are ordered in bundles in the b crystallographic direction of the P63/m space group of HA. Intermediate processes, e.g., dehydration of dicalcium phosphate, are critical for the formation of 1D HA alongside other key aspects of this phase transformation, it must be investigated in more detail in the continuous design of smart HA micro- and nano-structures with advanced therapeutic potentials. T2 - Materials Science and Engineering: C T1 - Hydrothermally processed 1D hydroxyapatite: Mechanism of formation and biocompatibility studies VL - 68 SP - 746 EP - 757 DO - 10.1016/j.msec.2016.06.047 ER -
@article{ author = "Stojanović, Zoran S. and Ignjatović, Nenad L. and Wu, Victoria and Žunič, Vojka and Veselinović, Ljiljana M. and Škapin, Srečo Davor and Miljković, Miroslav and Uskoković, Vuk and Uskoković, Dragan", year = "2016", abstract = "Recent developments in bone tissue engineering have led to an increased interest in one-dimensional (1D) hydroxyapatite (HA) nano- and micro-structures such as wires, ribbons and tubes. They have been proposed for use as cell substrates, reinforcing phases in composites and carriers for biologically active substances. Here we demonstrate the synthesis of 1D HA structures using an optimized, urea-assisted, high-yield hydrothermal batch process. The one-pot process, yielding HA structures composed of bundles of ribbons and wires, was typified by the simultaneous occurrence of a multitude of intermediate reactions, failing to meet the uniformity criteria over particle morphology and size. To overcome these issues, the preparation procedure was divided to two stages: dicalcium phosphate platelets synthesized in the first step were used as a precursor for the synthesis of 1D HA in the second stage. Despite the elongated particle morphologies, both the precursor and the final product exhibited excellent biocompatibility and caused no reduction of viability when tested against osteoblastic MC3T3-E1 cells in 2D culture up to the concentration of 2.6 mg/cm2. X-ray powder diffraction combined with a range of electron microscopies and laser diffraction analyses was used to elucidate the formation mechanism and the microstructure of the final particles. The two-step synthesis involved a more direct transformation of DCP to 1D HA with the average diameter of 37 nm and the aspect ratio exceeding 100:1. The comparison of crystalline domain sizes along different crystallographic directions showed no signs of significant anisotropy, while indicating that individual nanowires are ordered in bundles in the b crystallographic direction of the P63/m space group of HA. Intermediate processes, e.g., dehydration of dicalcium phosphate, are critical for the formation of 1D HA alongside other key aspects of this phase transformation, it must be investigated in more detail in the continuous design of smart HA micro- and nano-structures with advanced therapeutic potentials.", journal = "Materials Science and Engineering: C", title = "Hydrothermally processed 1D hydroxyapatite: Mechanism of formation and biocompatibility studies", volume = "68", pages = "746-757", doi = "10.1016/j.msec.2016.06.047" }
Stojanović, Z. S., Ignjatović, N. L., Wu, V., Žunič, V., Veselinović, L. M., Škapin, S. D., Miljković, M., Uskoković, V.,& Uskoković, D.. (2016). Hydrothermally processed 1D hydroxyapatite: Mechanism of formation and biocompatibility studies. in Materials Science and Engineering: C, 68, 746-757. https://doi.org/10.1016/j.msec.2016.06.047
Stojanović ZS, Ignjatović NL, Wu V, Žunič V, Veselinović LM, Škapin SD, Miljković M, Uskoković V, Uskoković D. Hydrothermally processed 1D hydroxyapatite: Mechanism of formation and biocompatibility studies. in Materials Science and Engineering: C. 2016;68:746-757. doi:10.1016/j.msec.2016.06.047 .
Stojanović, Zoran S., Ignjatović, Nenad L., Wu, Victoria, Žunič, Vojka, Veselinović, Ljiljana M., Škapin, Srečo Davor, Miljković, Miroslav, Uskoković, Vuk, Uskoković, Dragan, "Hydrothermally processed 1D hydroxyapatite: Mechanism of formation and biocompatibility studies" in Materials Science and Engineering: C, 68 (2016):746-757, https://doi.org/10.1016/j.msec.2016.06.047 . .