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dc.contributorUskoković, Dragan
dc.contributorRadmilović, Velimir R.
dc.creatorIgnjatović, Nenad L.
dc.creatorKuzmanović, Maja D.
dc.creatorPenov Gaši, Katarina
dc.creatorAjduković, Jovana
dc.creatorKojić, Vesna
dc.creatorUskoković, Dragan
dc.date.accessioned2018-03-06T10:00:50Z
dc.date.available2018-03-06T10:00:50Z
dc.date.issued2015
dc.identifier.urihttp://dais.sanu.ac.rs/123456789/826
dc.identifier.urihttp://www.itn.sanu.ac.rs/opus4/frontdoor/index/index/docId/1106
dc.identifier.urihttp://www.itn.sanu.ac.rs/opus4/files/1106/Ignjatovic_YUCOMAT-2015.pdf
dc.identifier.urihttp://vinar.vin.bg.ac.rs/handle/123456789/7542
dc.description.abstractIn our study, we examined the possibilities for the application of Thermo-Gravimetric Analysis/Differential-Thermal Analysis (DTA/TGA) coupled on-line with mass spectrometry (MS) as a fingerprint for identification purposes in drug loading processes. Androstane derivative 17β-hydroxy-17α-picolyl-androst-5-en-3β-yl acetate (2-OAc) with antitumor activity was loaded in nano hydroxyapatite (HAp) coated with chitosan-poly(D,L)-lactide-co-glycolide (Ch-PLGA) by emulsification and finally freeze-dried. By means of DTA/TGA-MS, it was quickly determined that the form of 2-OAc was the same before and after loading. The observed exothermic and endothermic processes due to the transformation of material with simultaneous analysis of gas products have proven to be successful in the analysis of drug loading processes in multi-component ceramic-polymer carriers. The loading efficiency of 74.7% was determined using the Differential Scanning Calorimetry (DSC) technique. A FT-IR analysis confirmed the qualitative composition of the synthesized 2-OAc-loaded HAp/Ch-PLGA. The in vitro antiproliferative activity was evaluated against human cell lines: lung adenocarcinoma (A549), as well as healthy fetal lung fibroblasts (MRC-5). The results of DET and MTT tests have revealed a high viability of healthy cells MRC-5 (82%) and the death of cancer cells A549 (46%) after a treatment with 2-OAc-loaded HAp/Ch-PLGA.en
dc.format(2015) 35-35
dc.formatapplication/pdf
dc.languageen
dc.publisherBelgrade : Materials Research Society of Serbia
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/45004/RS//
dc.rightsopenAccess
dc.sourceProgramme and The Book of Abstracts / Seventeenth Annual Conference YUCOMAT 205, Herceg Novi, August 31– September 4, 2015en
dc.subjectandrostane
dc.subjectchitosan-poly(D,L)-lactide-co-glycolide
dc.subjectthermo-gravimetric analysis
dc.subjectdifferential-thermal analysis
dc.subjecton-line mass spectrometry
dc.titleA Facile Determination Method for an Androstane-based Lung Cancer Inhibitor Loaded in Nano/Micro Particles Based on Hydroxyapatite by Means of DTA/TGA Coupled with On-line Mass Spectrometryen
dc.typeconferenceObject
dc.rights.licenseBY-NC-ND
dcterms.abstractAјдуковић, Јована; Игњатовић, Ненад; Пенов Гаши, Катарина; Кузмановић, Маја; Којић, Весна; Ускоковић, Драган;
dc.citation.spage35
dc.citation.epage35
dc.identifier.fulltexthttp://vinar.vin.bg.ac.rs//bitstream/id/14137/7536.pdf


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