Nanoparticles of cobalt-substituted hydroxyapatite in regeneration of mandibular osteoporotic bones
Аутори
Ignjatović, Nenad L.Ajduković, Zorica
Savić, Vojin
Najman, Stevo
Mihailović, Dragan
Vasiljević, Perica
Stojanović, Zoran S.
Uskoković, Vuk
Uskoković, Dragan
Чланак у часопису (Рецензирана верзија)
Метаподаци
Приказ свих података о документуАпстракт
Indications exist that paramagnetic calcium phosphates may be able to promote regeneration of bone faster than their regular, diamagnetic counterparts. In this study, analyzed was the influence of paramagnetic cobalt-substituted hydroxyapatite nanoparticles on osteoporotic alveolar bone regeneration in rats. Simultaneously, biocompatibility of the material was tested in vitro, on osteoblastic MC3T3-E1 and epithelial Caco-2 cells in culture. The material was shown to be biocompatible and nontoxic when added to epithelial monolayers in vitro, while it caused a substantial decrease in the cell viability as well as deformation of the cytoskeleton and cell morphology when incubated with the osteoblastic cells. In the course of 6 months after the implantation of the material containing different amounts of cobalt, ranging from 5 to 12 wt%, in the osteoporotic alveolar bone of the lower jaw, the following parameters were investigated: histopathological parameters, alkaline phosphatase and alv...eolar bone density. The best result in terms of osteoporotic bone tissue regeneration was observed for hydroxyapatite nanoparticles with the largest content of cobalt ions. The histological analysis showed a high level of reparatory ability of the nanoparticulate material implanted in the bone defect, paralleled by a corresponding increase in the alveolar bone density. The combined effect of growth factors from autologous plasma admixed to cobalt-substituted hydroxyapatite was furthermore shown to have a crucial effect on the augmented osteoporotic bone regeneration upon the implantation of the biomaterial investigated in this study.
Кључне речи:
bone regeneration / cobalt-substituted hydroxyapatite / nanoparticles / osteoporosisИзвор:
Journal of Materials Science: Materials in Medicine, 2013, 343-354Финансирање / пројекти:
- Молекуларно дизајнирање наночестица контролисаних морфолошких и физичко-хемијских карактеристика и функционалних материјала на њиховој основи (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-45004)
Напомена:
- This is accepted peer-reviewed version of the article: Ignjatović, N., Ajduković, Z., Savić, V., Najman, S., Mihailović, D., Vasiljević, P., ... & Uskoković, D. (2013). Nanoparticles of cobalt-substituted hydroxyapatite in regeneration of mandibular osteoporotic bones. Journal of Materials Science: Materials in Medicine, 24(2), 343-354. https://dx.doi.org/10.1007/s10856-012-4793-1
- Published version: https://vinar.vin.bg.ac.rs/handle/123456789/7410
DOI: 10.1007/s10856-012-4793-1
ISSN: 0957-4530
WoS: 000314775100008
Scopus: 2-s2.0-84878374725
Колекције
Институција/група
VinčaTY - JOUR AU - Ignjatović, Nenad L. AU - Ajduković, Zorica AU - Savić, Vojin AU - Najman, Stevo AU - Mihailović, Dragan AU - Vasiljević, Perica AU - Stojanović, Zoran S. AU - Uskoković, Vuk AU - Uskoković, Dragan PY - 2013 UR - https://vinar.vin.bg.ac.rs/handle/123456789/7481 AB - Indications exist that paramagnetic calcium phosphates may be able to promote regeneration of bone faster than their regular, diamagnetic counterparts. In this study, analyzed was the influence of paramagnetic cobalt-substituted hydroxyapatite nanoparticles on osteoporotic alveolar bone regeneration in rats. Simultaneously, biocompatibility of the material was tested in vitro, on osteoblastic MC3T3-E1 and epithelial Caco-2 cells in culture. The material was shown to be biocompatible and nontoxic when added to epithelial monolayers in vitro, while it caused a substantial decrease in the cell viability as well as deformation of the cytoskeleton and cell morphology when incubated with the osteoblastic cells. In the course of 6 months after the implantation of the material containing different amounts of cobalt, ranging from 5 to 12 wt%, in the osteoporotic alveolar bone of the lower jaw, the following parameters were investigated: histopathological parameters, alkaline phosphatase and alveolar bone density. The best result in terms of osteoporotic bone tissue regeneration was observed for hydroxyapatite nanoparticles with the largest content of cobalt ions. The histological analysis showed a high level of reparatory ability of the nanoparticulate material implanted in the bone defect, paralleled by a corresponding increase in the alveolar bone density. The combined effect of growth factors from autologous plasma admixed to cobalt-substituted hydroxyapatite was furthermore shown to have a crucial effect on the augmented osteoporotic bone regeneration upon the implantation of the biomaterial investigated in this study. T2 - Journal of Materials Science: Materials in Medicine T1 - Nanoparticles of cobalt-substituted hydroxyapatite in regeneration of mandibular osteoporotic bones SP - 343 EP - 354 DO - 10.1007/s10856-012-4793-1 ER -
@article{ author = "Ignjatović, Nenad L. and Ajduković, Zorica and Savić, Vojin and Najman, Stevo and Mihailović, Dragan and Vasiljević, Perica and Stojanović, Zoran S. and Uskoković, Vuk and Uskoković, Dragan", year = "2013", abstract = "Indications exist that paramagnetic calcium phosphates may be able to promote regeneration of bone faster than their regular, diamagnetic counterparts. In this study, analyzed was the influence of paramagnetic cobalt-substituted hydroxyapatite nanoparticles on osteoporotic alveolar bone regeneration in rats. Simultaneously, biocompatibility of the material was tested in vitro, on osteoblastic MC3T3-E1 and epithelial Caco-2 cells in culture. The material was shown to be biocompatible and nontoxic when added to epithelial monolayers in vitro, while it caused a substantial decrease in the cell viability as well as deformation of the cytoskeleton and cell morphology when incubated with the osteoblastic cells. In the course of 6 months after the implantation of the material containing different amounts of cobalt, ranging from 5 to 12 wt%, in the osteoporotic alveolar bone of the lower jaw, the following parameters were investigated: histopathological parameters, alkaline phosphatase and alveolar bone density. The best result in terms of osteoporotic bone tissue regeneration was observed for hydroxyapatite nanoparticles with the largest content of cobalt ions. The histological analysis showed a high level of reparatory ability of the nanoparticulate material implanted in the bone defect, paralleled by a corresponding increase in the alveolar bone density. The combined effect of growth factors from autologous plasma admixed to cobalt-substituted hydroxyapatite was furthermore shown to have a crucial effect on the augmented osteoporotic bone regeneration upon the implantation of the biomaterial investigated in this study.", journal = "Journal of Materials Science: Materials in Medicine", title = "Nanoparticles of cobalt-substituted hydroxyapatite in regeneration of mandibular osteoporotic bones", pages = "343-354", doi = "10.1007/s10856-012-4793-1" }
Ignjatović, N. L., Ajduković, Z., Savić, V., Najman, S., Mihailović, D., Vasiljević, P., Stojanović, Z. S., Uskoković, V.,& Uskoković, D.. (2013). Nanoparticles of cobalt-substituted hydroxyapatite in regeneration of mandibular osteoporotic bones. in Journal of Materials Science: Materials in Medicine, 343-354. https://doi.org/10.1007/s10856-012-4793-1
Ignjatović NL, Ajduković Z, Savić V, Najman S, Mihailović D, Vasiljević P, Stojanović ZS, Uskoković V, Uskoković D. Nanoparticles of cobalt-substituted hydroxyapatite in regeneration of mandibular osteoporotic bones. in Journal of Materials Science: Materials in Medicine. 2013;:343-354. doi:10.1007/s10856-012-4793-1 .
Ignjatović, Nenad L., Ajduković, Zorica, Savić, Vojin, Najman, Stevo, Mihailović, Dragan, Vasiljević, Perica, Stojanović, Zoran S., Uskoković, Vuk, Uskoković, Dragan, "Nanoparticles of cobalt-substituted hydroxyapatite in regeneration of mandibular osteoporotic bones" in Journal of Materials Science: Materials in Medicine (2013):343-354, https://doi.org/10.1007/s10856-012-4793-1 . .